양측 난소절제술을 시행한 암컷 백서의 방광에서 나타나는 단백질의 변화
Proteomic Changes in the Female Rat Bladder Tissue following Bilateral Oophorectomy
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Purpose: To explore the effect of bilateral oophorectomy on the several proteins of female rat bladder via a proteomic approach. Materials and Methods: A total of 20 female Sprague-Dawley rats were obtained at 8 weeks of age and were divided randomly into 3 groups: a control groups underwent sham operation and bladders were excised 4 weeks and 8 weeks after operation each. Other two groups underwent bilateral oophorectomy and bladders were excised 4 weeks (group 1) and 8 weeks (group 2) after operation each, too. Each group has 5 rats. Conventional proteomics was conducted via high resolution 2-D gel electrophoresis, followed by image analysis and protein identification through mass spectrometry. Results: Bladder weights were reduced significantly in group 2 as compared with the 8-week control group. A comparison of the bladders of the oophorectomy group subjects with those of the control group subjects demonstrated that the expressions of 11 proteins were altered Eukaryotic translation initiation factor 5A (elF-5A), chaperone grp 75 precursor, actin-depolymerizing factor, contrapsin-like inhibitor 1 precursor, guanine deaminase, actin, peroxiredoxin 2, phosphatidylethanolamine, putative protein kinase, Keratin complex 2 and Enol protein. Conclusion: The roles of 11 proteins are variable. A part of these proteins have a role of cellular apoptosis, acute inflammation, and muscle contraction. It seems that these changes of proteins have an influence on bladder functions and LUTS.
Abstract
Purpose: To explore the effect of bilateral oophorectomy on the several proteins of female rat bladder via a proteomic approach. Materials and Methods: A total of 20 female Sprague-Dawley rats were obtained at 8 weeks of age and were divided randomly into 3 groups: a control groups underwent sham operation and bladders were excised 4 weeks and 8 weeks after operation each. Other two groups underwent bilateral oophorectomy and bladders were excised 4 weeks (group 1) and 8 weeks (group 2) after operation each, too. Each group has 5 rats. Conventional proteomics was conducted via high resolution 2-D gel electrophoresis, followed by image analysis and protein identification through mass spectrometry. Results: Bladder weights were reduced significantly in group 2 as compared with the 8-week control group. A comparison of the bladders of the oophorectomy group subjects with those of the control group subjects demonstrated that the expressions of 11 proteins were altered Eukaryotic translation initiation factor 5A (elF-5A), chaperone grp 75 precursor, actin-depolymerizing factor, contrapsin-like inhibitor 1 precursor, guanine deaminase, actin, peroxiredoxin 2, phosphatidylethanolamine, putative protein kinase, Keratin complex 2 and Enol protein. Conclusion: The roles of 11 proteins are variable. A part of these proteins have a role of cellular apoptosis, acute inflammation, and muscle contraction. It seems that these changes of proteins have an influence on bladder functions and LUTS.
참고문헌 (24)
- Cardozo L, "Role of estrogens in the treatment of female urinary incontinence", J Am Geriatr Soc, 38, pp.326~328 (1990)
- Iosif CS, "Estrogen receptors in the human female lower urinary tract", Am J Obstet Gynecol, 141, pp.817~820 (1981)
- Batra SC, "Female urethra: a target for estrogen action", J Urol, 129, pp.418~420 (1983)
- Batra SC, "Progesterone receptors in the female lower urinary tract", J Urol, 138, pp.1301~1304 (1987)
- Eika B, "Long-term observation of the detrusor smooth muscle in rats. its relationship to ovariectomy and estrogen treatment", Urol Res, 18, pp.439~442 (1990)
- Blakeman PJ, "Oestrogen and progesterone receptor expression in the female lower urinary tract, with reference to oestrogen status", BJU Int, 86, pp.32~38 (2000)
- Shapiro E, "Effect of estrogens on the weight and muscarinic cholinergic receptor density of the rabbit bladder and urethra", J Urol, 135, pp.1084~1087 (1986)
- Batra S, "Oestrogen-induced changes in muscarinic receptor density and contractile responses in the female rabbit urinary bladder", Acta Physiol Scand, 137, pp.135~141 (1989)
- Judd HL, "Estrogen replacement therapy", Obstet Gynecol, 58, pp.267~275 (1981)
- Fantl JA, "Postmenopausal urinary incontinence: comparison between non-estrogen-supplemented and estrogen-supplemented women", Obstet Gynecol, 71, pp.823~828 (1988)
- Robinson D, "The role of estrogens in female lower urinary tract dysfunction", Urology, 62, pp.45~51 (2003)
- Parekh MH, "Effects of castration on female rabbit bladder physiology and morphology", Urology, 64, pp.1048~1051 (2004)
- Kim HJ, "Preliminary report of short-term proteomic changes following bilateral oophorectomyin the female rat bladder tissue", J Korean Continence Soc, 10, pp.121~125 (2006)
- Taylor CA, "Cliche DO, Boone AN, et al. Role of eIF5A in TNF-alpha-mediated apoptosis of lamina cribrosa cells", Invest Ophthalmol Vis Sci, 45, pp.3568~3576 (2004)
- Willis D, "Differential transport and local translation of cytoskeletal, injury-response, and neurodegeneration protein mRNAs in axons", J Neurosci, 25, pp.778~791 (2005)
- Kelly S, "Gene transfer of HSP72 protects cornu ammonis 1 region of the hippocampus neurons from global ischemia: influence of Bcl-2", Ann Neurol, 52, pp.160~167 (2002)
- Kalwy SA, "Heat shock protein 27 delivered via a herpes simplex virus vector can protect neurons of the hippocampus against kainic-acid-induced cell loss", Brain Res Mol Brain Res, 111, pp.91~103 (2003)
- Olive M, "Desmin-related myopathy: clinical, electrophysiological, radiological, neuropathological and genetic studies", J Neurol Sci, 219, pp.125~137 (2004)
- Pages G, "Molecular characterization of three rat liver serine-protease inhibitors affected by inflammation and hypophysectomy. Protein and mRNA analysis and cDNA cloning", Eur J Biochem, 190, pp.385~391 (1990)
- Miyamoto S, "Guanine deaminase from rat brain. Purification, characteristics, andcontribution to ammoniagenesis in the brain", J Biochem, 91, pp.167~176 (1982)
- Dietz KJ, "The function of the chloroplast 2-cysteine peroxiredoxin in peroxide detoxification and its regulation", J Exp Bot, 53, pp.1321~1329 (2002)
- Vergara J, "Isolation and characterization of luminal membranes from urinary bladder", J Cell Biol, 61, pp.83~94 (1974)
- Celis JE, "Proteomics and immunohistochemistry define some of the steps involved in the squamous differentiation of the bladder transitional epithelium: a novel strategy for identifying metaplastic lesions", Cancer Res, 59, pp.3003~3009 (1999)
- Butterfield DA, "Redox proteomics identification of oxidatively modified hippocampal proteins in mild cognitive impairment: insights into the development of Alzheimer's disease", Neurobiol Dis, 22, pp.223~232 (2006)