조위승청탕의 알코올 및 熱水 추출물이 지방세포 대사에 미치는 영향
Effects of Chowiseungcheng-tang Extracts on the Preadipocytes Proliferation in 3T3-L1 cell line, Lipolysis of Adipocytes in rat, and Localized Fat Accumulation by extraction methods
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Objectives : The purpose of this study is to investigate the effects of Chowiseungcheng-tang extracts on the preadipocytes proliferation in 3T3-L1 cell line, lipolysis of adipocytes in rat's epididymal adipocytes and localized fat accumulation of porcine by extraction methods(alcohol and water). Methods : Diminish preadipocytes proliferation and promote lipolysis of adipocytes do primary role to reduce obesity. So, we used 3T3-L1 mouse embryo fibroblasts(preadipocytes) and rat epididymal adipocytes from Sprague-Dawley rats to investigate the effects of Chowiseungcheng-tang extracts on the preadipocytes proliferation, lipolysis of adipocytes. They were treated with 0.01, 0.1, 1.0 ㎎/㎖ Chowiseungcheng-tang alcohol and water extracts. And for the purpose of investigating the effects of Chowiseungcheng-tang alcohol and water extracts on the localized fat accumulation, we injected 0.1, 1.0, 10.0 ㎎/㎖ Chowiseungcheng-tang extracts to porcine fat tissues and observed histological changes of them. Results : Following results were obtained from the preadipocytes proliferation and lipolysis of adipocytes and histological investigation of fat tissues. 1. Chowiseungcheng-tang extracts suppressed preadipocytes proliferation on the high dosage(especially 1.0 ㎎/㎖), and especially alcohol extracts had better effects. 2. The alcohol extracts of Chowiseungcheng-tang decreased the activity of glycerol-3-phosphate dehydrogenase(GPDH) on the concentrations of 0.1, 1.0 ㎎/㎖. Alcohol extracts had better effects than water extracts. 3. Chowiseungcheng-tang extracts increased lipolysis of adipocytes on the concentrations of 0.1, 1.0 ㎎/㎖, and especially on the concentration of 1.0 ㎎/㎖ alcohol extract of Chowiseungcheng-tang had better effect. 4. The water extract of Chowiseungcheng-tang had significant activity to the destruction of porcine fat cell membranes only on the concentration of 10.0 ㎎/㎖, but alcohol extracts of Chowiseungcheng-tang had it on all concentrations. Conclusions : The alcohol extracts of Chowiseungcheng-tang had much better effects on the preadipocytes proliferation, lipolysis of adipocytes and localized fat accumulation than water extracts.
Abstract
Objectives : The purpose of this study is to investigate the effects of Chowiseungcheng-tang extracts on the preadipocytes proliferation in 3T3-L1 cell line, lipolysis of adipocytes in rat's epididymal adipocytes and localized fat accumulation of porcine by extraction methods(alcohol and water). Methods : Diminish preadipocytes proliferation and promote lipolysis of adipocytes do primary role to reduce obesity. So, we used 3T3-L1 mouse embryo fibroblasts(preadipocytes) and rat epididymal adipocytes from Sprague-Dawley rats to investigate the effects of Chowiseungcheng-tang extracts on the preadipocytes proliferation, lipolysis of adipocytes. They were treated with 0.01, 0.1, 1.0 ㎎/㎖ Chowiseungcheng-tang alcohol and water extracts. And for the purpose of investigating the effects of Chowiseungcheng-tang alcohol and water extracts on the localized fat accumulation, we injected 0.1, 1.0, 10.0 ㎎/㎖ Chowiseungcheng-tang extracts to porcine fat tissues and observed histological changes of them. Results : Following results were obtained from the preadipocytes proliferation and lipolysis of adipocytes and histological investigation of fat tissues. 1. Chowiseungcheng-tang extracts suppressed preadipocytes proliferation on the high dosage(especially 1.0 ㎎/㎖), and especially alcohol extracts had better effects. 2. The alcohol extracts of Chowiseungcheng-tang decreased the activity of glycerol-3-phosphate dehydrogenase(GPDH) on the concentrations of 0.1, 1.0 ㎎/㎖. Alcohol extracts had better effects than water extracts. 3. Chowiseungcheng-tang extracts increased lipolysis of adipocytes on the concentrations of 0.1, 1.0 ㎎/㎖, and especially on the concentration of 1.0 ㎎/㎖ alcohol extract of Chowiseungcheng-tang had better effect. 4. The water extract of Chowiseungcheng-tang had significant activity to the destruction of porcine fat cell membranes only on the concentration of 10.0 ㎎/㎖, but alcohol extracts of Chowiseungcheng-tang had it on all concentrations. Conclusions : The alcohol extracts of Chowiseungcheng-tang had much better effects on the preadipocytes proliferation, lipolysis of adipocytes and localized fat accumulation than water extracts.
참고문헌 (50)
- 全國韓醫科大學 再活醫學科學敎室, "東醫再活醫學科學", pp.570~585 (1995), 書苑堂
- Diehle HS, "Obesity and Digestive disorder and disease. Healthful living", pp.65~68 (1973), McGraw-Hill
- "National Heart, Lung, and Blood Institute in cooperation with The National Institute of Diabetes and Digestive and Kidney Diseases. Clinical Guidelines on the Identification, Evaluation, and Treatment of Overweight and Obesity in Adults//The Evidence Report: National Institute of Health/1998"[URL]
- "Resource Guide for Nutrition and Physical Activity Interventions to Prevent Obesity and Other Chronic Diseases//CDC/"[URL]
- 탁양주, "최근 국내 비만 연구의 경향: 1984년부터 2002년까지", 대한비만학회지, 13(1), pp.1~13 (2004)
- 박인상, "東醫四象要訣", pp.156~ (1992), 소나무
- 신미숙, "調胃升淸湯 및 그 加味方이 비만마우스의 체중과 혈청 함량 변화에 미치는 영향", 한방재활의학과학회지, 12(1), pp.157~166 (2002)
- 서동민, "비만 환자에 대한 조위승청탕의 효과 및 부작용에 관한 임상적 관찰", 대한침구의학회지, 22(3), pp.145~154 (2005), 대한침구의학회
- 李濟馬, "東醫壽世保元", pp.7~123 (1986), 행림출판사
- 류주열, "東醫四象醫學講座", pp.631~638 (2003), 대성의학사
- 보건복지부, "한약재 수급 및 유통관리 규정 제 2조 2항" (2006), 보건복지부고시
- Singh R., "Testosterone inhibits adipogenic differentiation in 3T3-L1 cells: Nuclear translocation of androgen receptor complex with β-catenin and T-cell factor 4 may bypass canonical Wnt signaling to down-regulate adipogenic transcription factors", Endocrinology, 147, pp.141~154 (2006)
- Negrel R., "Culture of adipose precursor cells and cells of clonal lines from animal white adipose tissue. In Adipose Tissue Protocols", pp.225~237 (2001), Human Press
- Wise LS., "Participation of one isozyme of cytosolic glycerophosphate dehydrogenase in the adipose conversion of 3T3 cells", J. Biol. Chem, 254, pp.273~275 (1979)
- Bradford MM., "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding", Anal. Biochem, 72, pp.248~254 (1976)
- Rodbell M., "Metabolism of isolated fat cells. I. Effects of hormones on glucose metabolism and lipolysis", J. Biol. Chem, 239, pp.375~380 (1964)
- Zalatan F., "Inhibition of isoproterenol -induced lipolysis in rat inguinal adipocytes in vitro by physiological melatonin via a receptor-mediated mechanism", Endocrinology, 142, pp.3783~3790 (2001)
- McGowan MW., "A peroxidase -coupled method for the colorimetric determination of serum triglycerides", Clin. Chem, 29, pp.538~542 (1983)
- Steel RGD., "Principles and Procedures of Statistics", pp.187~188 (1980), McGraw-Hill
- 이광우, "비만증의 진단과 평가", 대한비만학회지, 1(1), pp.1~4 (1992)
- 대한비만학회, "임상 비만학", pp.19~28 (2001), 고려의학
- 김영설, "비만증의 진단과 평가", 대한비만학회지, 11(2), pp.3~8 (2002)
- Fontbonne A., "Body fat distribution and coronary heart disease mortality in subjects with impaired glucose tolerance or diabetes mellitus: the Paris Prospective Study, 15-year follow up", Diabetologia, 35, pp.464~468 (1992)
- Folsom AR., "Body fat distribution and 5-year risk of death in older women", Journal of the Amerian Medical Association, 269, pp.483~487 (1993)
- Kalmijn S., "The association of body weight and anthropometry with mortality in elderly men: The Honolulu Heart Program", International Journal of Obesity, 23, pp.395~402 (1999)
- Reaven GM., "Role of insulin resistance in human disease", Diabetes, 37, pp.1595~1607 (1988)
- Haffner SM., "Prospective analysis of the insulin resistance syndrome", Diabetes, 41, pp.715~722 (1992)
- Grundy SM., "Hypertriglyceridemia, insulin resistance, and the metabolic syndrome", Am J Cardiol, 83, pp.25~29 (1999)
- Kissebah AH., "Regional adiposity and morbidity", Physiological Reviews, 74, pp.761~811 (1994)
- 王 冰, "黃帝內經靈樞", pp.155~209 (1972), 臺灣中華書局
- 李 梴, "編註醫學入門", pp.108~278 (1990), 大星文化社
- 陳貴延, "實用中西醫結合診斷治療學", pp.682~689 (1991), 中國醫藥科學技術出版社
- 김달래, "四象體質과 肥滿의 상관성에 관한 임상적 연구", 사상의학회지, 8(1), pp.319~335 (1996)
- 조홍건, "肥滿症의 原因과 病機 및 治法에 관한 文獻的 考察", 대전대학교 한의학연구소 논문집, 1(2), pp.61~71 (1992)
- 신현대, "肥滿의 治療에 關한 臨床的 比較 硏究", 대한한의학회지, 13(2), pp.63~73 (1992)
- 박상용, "肥滿 治療에 대한 鍼灸 및 藥物治療의 臨床的 硏究", 大田大學校 論文集 韓醫學編, 2(2), pp.163~185 (1994)
- 정선희, "肥滿患者의 電鍼治療 臨床例", 대한침구학회지, 16(3), pp.39~56 (1999)
- 김연섭, "비만 치료 및 예방에 대한 한약의 효능 연구", 대한본초학회지, 15(1), pp.73~82 (2000)
- 윤대환, "高脂肪食餌로 誘發된 肥滿白鼠모델에서 蒼朮, 薏苡仁, 麻黃, 蒼朮合薏苡仁, 麻黃合綠茶 藥鍼이 血淸脂質 및 肝機能에 미치는 影響", 제 3회 대한약침학회 학술대회 논문집, 7(1), pp.87~100 (2004)
- 지준환, "赤小豆蒸溜藥鍼이 肥滿쥐의 脂質構成, 肝臟機能, 抗酸化效果 및 分子生物學的 特性에 미치는 影響", 대한약침학회지, 8(2), pp.67~82 (2005), 대한약침학회
- 조희철, "웅담과 우황약침이 지방세포 대사에 미치는 영향", 대한침구의학회지, 24(4), pp.125~142 (2007), 대한침구의학회
- 정영표, "胃兪의 山査 藥鍼이 高脂肪 食餌로 誘發된 肥滿白鼠에 미치는 影響", 대한침구의학회지, 24(4), pp.55~68 (2007), 대한침구의학회
- 강수우, "脾兪에 대한 羅蔔子 藥鍼이 고지방 식이로 유발된 肥滿白鼠에 미치는 영향", 대한침구학회지, 24(5), pp.113~125 (2007)
- 주집성, "肥滿 治療를 위한 經穴 및 藥物에 관한 考察", 대한경락경혈학회지, 20(3), pp.147~162 (2003)
- 김호철, "韓藥藥理學", pp.63~66 (2001), 集文堂
- Astrup A., "Ephedrine and weight loss", Int J Obes Relat Metab Disord, 16(9), pp.715~ (1992)
- Green H., "Sublines of mouse 3T3 cells that accumulate lipid", Cell, 1, pp.113~116 (1974)
- Green H., "An established preadipose cell line and its differentiation in culture", Cell, 3, pp.127~133 (1974)
- Green H., "An established preadipose cell line and its differentiation in culture Ⅱ. Factors affecting the adipose conversion", Cell, 5, pp.19~27 (1975)
- Green H., "Spontaneous heritable changes leading to increased adipose conversion", Cell, 7, pp.105~113 (1976)
