본문 바로가기
KERD
논문KCI 등재

당귀약침액이 대식세포에서 산화질소(NO) 및 프로스타글란딘 (Prostaglandin) 생성에 미치는 영향

Effects of Angelicae Gigantis Radix pharmacopuncture on Nitric Oxide and Prostaglandin E2 Production in Macrophage.

1

조회

0

다운로드

10

인용

0

참고문헌

키워드

주제분류한의학

초록

Objectives : Angelicae Gigantis Radix has been known traditional medicine with antimicrobial activities and it has been widely used for treatment of blood and inflammatory diseases. In the present study, some studies examined anti-inflammation effects of Angelicae Gigantis Radix but they usually were performed by ethanol extracted Angelicae Gigantis Radix pharmacopuncture. So We investigated the inhibitory effects of Angelicae Gigantis Radix pharmacopuncture by hot water and ethanol extract on Nitric oxide(NO) and Prostaglandin E2(PGE2) production in lipopolysaccharide(LPS)induced macrophage cell. Methods : Angelicae Gigantis Radix was extracted by ethanol and hot water. Cell viability was determined by MTT assay. To evaluate anti-inflammation effects of Angelicae Gigantis Radix pharmacopuncture,we examined NO and PGE2 production in LPS induced macrophages. The concentrations of NO and PGE2 were measured by Griess assay and Enzyme Immuno-Assay. Results :1) The MTT assay demonstrated that cytotoxic effect of Angelicae Gigantis Radix pharmacopuncture by hot water extract and ethanol extract in RAW 264.7 macrophage cells were not appeared. 2) Angelicae Gigantis Radix pharmacopuncture by ethanol extract and hot water extract inhibited NO production in LPS induced macrophages significantly. 3) Angelicae Gigantis Radix pharmacopuncture by ethanol extract tended to inhibiting PGE2 production in LPS induced macrophages. And Angelicae Gigantis Radix pharmacopuncture by hot water extract inhibited LPS induced production of PGE2 in RAW 264.7 macrophage cells significantly. Conclusions : This study suggests that Angelicae Gigantis Radix pharmacopuncture may have an anti-inflammatory property through the inhibition of NO and PGE2 production in LPS induced macrophages. It may have a therapeutic potential for the treatment of various inflammatory diseases.

Abstract

Objectives : Angelicae Gigantis Radix has been known traditional medicine with antimicrobial activities and it has been widely used for treatment of blood and inflammatory diseases. In the present study, some studies examined anti-inflammation effects of Angelicae Gigantis Radix but they usually were performed by ethanol extracted Angelicae Gigantis Radix pharmacopuncture. So We investigated the inhibitory effects of Angelicae Gigantis Radix pharmacopuncture by hot water and ethanol extract on Nitric oxide(NO) and Prostaglandin E2(PGE2) production in lipopolysaccharide(LPS)induced macrophage cell. Methods : Angelicae Gigantis Radix was extracted by ethanol and hot water. Cell viability was determined by MTT assay. To evaluate anti-inflammation effects of Angelicae Gigantis Radix pharmacopuncture,we examined NO and PGE2 production in LPS induced macrophages. The concentrations of NO and PGE2 were measured by Griess assay and Enzyme Immuno-Assay. Results :1) The MTT assay demonstrated that cytotoxic effect of Angelicae Gigantis Radix pharmacopuncture by hot water extract and ethanol extract in RAW 264.7 macrophage cells were not appeared. 2) Angelicae Gigantis Radix pharmacopuncture by ethanol extract and hot water extract inhibited NO production in LPS induced macrophages significantly. 3) Angelicae Gigantis Radix pharmacopuncture by ethanol extract tended to inhibiting PGE2 production in LPS induced macrophages. And Angelicae Gigantis Radix pharmacopuncture by hot water extract inhibited LPS induced production of PGE2 in RAW 264.7 macrophage cells significantly. Conclusions : This study suggests that Angelicae Gigantis Radix pharmacopuncture may have an anti-inflammatory property through the inhibition of NO and PGE2 production in LPS induced macrophages. It may have a therapeutic potential for the treatment of various inflammatory diseases.

참고문헌 (39)

  1. 전국한의과대학 병리학교실, "한방병리학", pp.109~112 (2004), 一中社
  2. Abul K.Abbas, "Cellular and Molecular Immunology. 6th edition", pp.75~97 (2006), Saunders Elsevier
  3. Guo LY, "Anti-inflammatory effects of schisandrin isolated from the fruit of Schisandra chinensis Baill", Eur J Pharmacol, 591, pp.293~299 (2008)
  4. 김대희, "LPS로 활성화된 대식세포에서 黃連解毒湯 물추출물의 염증매개물질 억제효과", 대한본초학회지, 24(4), pp.39~47 (2009), 대한본초학회
  5. 배기상, "곽향 추출물의 염증성세포활성물질 억제효과", 대한본초학회지, 23(3), pp.119~125 (2008), 대한본초학회
  6. 윤용갑, "금은화 수용성 추출물의 LPS 유도 염증매개물 억제 효과", 대한본초학회지, 22(3), pp.117~125 (2007), 대한본초학회
  7. 박성주, "LPS로 활성화된 복강 대식세포에서 백두옹 추출물의 항염증 효과", 대한본초학회지, 22(1), pp.111~125 (2007), 대한본초학회
  8. 전국한의과대학 공동편찬위원회, "본초학", pp.631~634 (2005), 영림사
  9. 박쾌한, "당귀수침이 진통효과에 미치는 영향에 관한 연구", 석사, pp.1984
  10. Hwang HS, "The effects of Angelicae gigantis Radix Aqua-acupuncture by Density on immune response induced by radiation in Mice", The Journal of Korean Acupuncture and Moxibustion Society, 11(4), pp.113~129 (1994)
  11. Moon JY, "A Study of the Effects of Moxibustion and A.G.R aqua-acupuncture at Ganshu(BL19) and Qimen(LR14) on Hyperlipidemia Induced by Cholesterol in Rats", The Journal of Korean Acupuncture and Moxibustion Society, 12(2), pp.119~134 (1995)
  12. Sung IW, "The effects of Aquaacupuncture of Hyunsoshang and Dangghuimi on Thrombosis Induced by Endotoxin in Rats", The Journal of Korean Acupuncture and Moxibustion Society, 11(1), pp.391~404 (1994)
  13. 신선희, "참당귀 에탄올 추출물의 항염증효과", 박사, pp.2008
  14. 이경용, "LPS로 활성화된 RAW 264.7 세포에서 당귀에탄올 추출물의 항염증효과", 석사, pp.2003
  15. "Inhibitory Effect of Angelica gigas Nakai Extract on Nitric Oxide Production in RAW 264.7 Cells", The Journal of Korean Oriental Medicine Society, 28(2), pp.138~148 (2007)
  16. The Korean Society of Pathologists, "Text of Pathology", pp.83~89 (1995), Komoonsa
  17. Mori M, "Regulation of nitric oxide synthesis and apoptosis by arginine recycling", J Nutr, 137, pp.1616~1620 (2007)
  18. Bae SC, "Nitric oxide(NO) in Inflammatory Arthritis", The Journal of Korean Orienatal Internal Medicine Society, 52(1), pp.32~41 (1997)
  19. "The Role of Spinal NO on the Mechanical Allodynia in the Inflammatioa Model by Freund`s Complete Adjuvant", The Journal of the Korean Society of Anesthesiologists, 38(6), pp.1068~1074 (2000)
  20. Kuo CL, "The anti-inflammatory potential of berberine in vitro and in vivo", Cancer Lett, 203, pp.127~137 (2004)
  21. Lee DU, "Effects of 13- alkyl-substituted berberine alkaroids on the expression of COX-II, TNF-alpha, iNOS, and IL-12 production in LPS-stimulated macrophages", Life Sci, 73, pp.1401~1412 (2003)
  22. Kim KW, "Folygonum cuspidatum, compared with baicalin and berberine, inhibits inducible nitric oxide synthase and cyclooxygenase-2 gene expressions in RAW 264.7 macrophages", Vascul Pharmacol, 47, pp.99~107 (2007)
  23. Hong JW, "Study on the Anti-inflammatory and Anti-thrombotic Effects of Bibangtalmyungsan in the experimental animal", The Korean Journal of Oriental Medical Pathology, 12(2), pp.63~72 (1998)
  24. 김철홍, "활락탕의 NO 억제, DPPH 소거 및 Microarray상 유전자 발현에 미치는 영향", 대한침구의학회지, 21(5), pp.69~78 (2004), 대한침구의학회
  25. 송호준, "LPS로 활성화된 복강 대식세포에서 세신 추출물의 항염증 효과", 대한본초학회지, 21(2), pp.189~195 (2006), 대한본초학회
  26. 박희제, "LPS로 자극한 RAW264.7 세포에서 강활 추출물의 염증성세포활성물질의 억제효과", 대한본초학회지, 23(3), pp.127~134 (2008), 대한본초학회
  27. 김성윤, "연교 추출물의 Microglia에서 LPS에 의해 유도되는 염증매개물질 생성 억제 효과", 대한본초학회지, 23(3), pp.93~102 (2008), 대한본초학회
  28. 박용기, "황련의 쥐 대식세포로부터 LPS에 의해 유도되는 nitric oxide 및 TNF-α의 생성억제효과", 대한본초학회지, 21(2), pp.165~173 (2006), 대한본초학회
  29. 이현진, "녹용약침이 백서의 제2형 Collagen 유발 관절염에서 iNOS 발현과 NO 생성 억제에 미치는 영향", 대한침구의학회지, 25(5), pp.105~116 (2008), 대한침구의학회
  30. 민지영, "續斷의 RAW264.7 세포에서 LPS에 의해 유도되는 염증반응에 대한 효과", 대한본초학회지, 24(4), pp.189~195 (2009), 대한본초학회
  31. 김동현, "白何首烏藥鍼液의 NO, DPPH 消去 및 IL-4 抑制效果", 대한침구의학회지, 20(4), pp.7~ (2003), 대한침구의학회
  32. 이경민, "黃耆藥鍼液의 NO, DPPH 消去 및 IL-4 抑制效果", 대한침구의학회지, 20(2), pp.15~ (2003), 대한침구의학회
  33. 박용기, "椒皮의 RAW264.7세포에서의 LPS에 의해 유도되는 nitric oxide 및 전염증사이토카인 생성억제효과", 대한본초학회지, 21(4), pp.69~76 (2006), 대한본초학회
  34. Ryu EJ, "Effects of Angelica gigas Nakai Aqua-acupuncture on the Ovarian Function", The Journal of Korean Acupuncture and Moxibustion Society, 15(1), pp.305~315 (1998)
  35. Ahn JC, "Antioxidant effects of A.G.R.(Angelicae gigantis Radix) Aquaacupuncture solution in vitro", The Journal of Korean Acupuncture and Moxibustion Society, 13(2), pp.254~262 (1996)
  36. 한상균, "당귀약침의 혈해 자입이 Intraluminal Filament 삽입술에 의해 유발된 백서의 허혈성 뇌손상에 미치는 영향", 대한침구의학회지, 21(2), pp.1~20 (2004), 대한침구의학회
  37. 장소영, "當歸藥鍼이 소음 stress를 받은 새끼 쥐의 신경세포 생성에 미치는 영향", 대한침구의학회지, 23(3), pp.47~56 (2006), 대한침구의학회
  38. 류미선, "足三里(ST36)․腎兪(BL23)에 대한 당귀약침이 Freund’s Adjuvant로 유발된 白鼠의 관절염에 미치는 영향", 대한침구의학회지, 27(5), pp.25~34 (2010), 대한침구의학회
  39. Kim KS, "Effects of Aqua-Acupuncture with RADIX ANGELICAE GIGANTIS and RADIX ASTRAGALI on the Blood in the Liver Damage Rats with CCl4", The Journal of Korean Acupuncture and Moxibustion Society, 13(1), pp.1~10 (1996)

관련 논문AI추천

이 간행물의 다른 논문 (8건)