ODAM과 BMPRIB가 법랑질의 석회화에 미치는 영향
Effect of ODAM and BMPRIB on Enamel Mineralization
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The purpose of this study was to investigate the biological function of ODAM and its signal transduction pathway in the steps of ameloblast differentiation and enamel mineralization. An ODAM recombinant protein was produced and stable ODAM transgenic cell lines were also established using ameloblast-lineage cells (ALCs). To verify the ODAM signal transduction pathway, BAMBI recombinant protein, an inhibitor of BMP2 and BMP receptor 1B (BMPR-1B), was treated and BMPR-1B siRNA was used to silence expression of BMPR-1B. Mineralization was augmented by the ALCs treated with the ODAM recombinant protein and the sense ODAM overexpressing cells. The ALP activity was also increased markedly in the sense ODAM overexpressing cells and the ALCs treated with ODAM recombinant protein. The inactivation of ODAM in the ALCs down-regulated the expression of BMPR-1B, whereas its expression was up-regulated markedly when ODAM was overexpressed. These results provide deeper insights into the process of ameloblast maturation and in enamel mineralization. It also suggested that ODAM augmented enamel mineralization.
Abstract
The purpose of this study was to investigate the biological function of ODAM and its signal transduction pathway in the steps of ameloblast differentiation and enamel mineralization. An ODAM recombinant protein was produced and stable ODAM transgenic cell lines were also established using ameloblast-lineage cells (ALCs). To verify the ODAM signal transduction pathway, BAMBI recombinant protein, an inhibitor of BMP2 and BMP receptor 1B (BMPR-1B), was treated and BMPR-1B siRNA was used to silence expression of BMPR-1B. Mineralization was augmented by the ALCs treated with the ODAM recombinant protein and the sense ODAM overexpressing cells. The ALP activity was also increased markedly in the sense ODAM overexpressing cells and the ALCs treated with ODAM recombinant protein. The inactivation of ODAM in the ALCs down-regulated the expression of BMPR-1B, whereas its expression was up-regulated markedly when ODAM was overexpressed. These results provide deeper insights into the process of ameloblast maturation and in enamel mineralization. It also suggested that ODAM augmented enamel mineralization.
참고문헌 (23)
- Solomon A, "Calcifying epithelial odontogenic(Pindborg)tumor-associated amyloid consists of a novel human protein", J Lab Clin Med, 142, pp.348~355 (2003)
- Aung PP, "Systematic search for gastric cancer-specific genes based on SAGE data:melanoma inhibitory activity and matrix metalloproteinase-10 are novel prognostic factors in patients with gastric cancer", Oncogene, 25, pp.2546~2557 (2006)
- Moffatt P, "Identification of secreted and membrane proteins in the rat incisor enamel organ using a signal-trap screening approach", Eur J Oral Sci, 114(1), pp.139~146 (2006)
- Park JC, "The amyloid protein ODAM is highly expressed during enamel mineralization and maturation in rat incisors", Eur J Oral Sci, 115, pp.153~160 (2007)
- Moffatt P, "Characterization of Apin,a secreted protein highly expressed in tooth-associated epithelia", J Cell Biochem, 103(3), pp.941~956 (2008)
- 박주철, "Protein microarray를 이용한 Apin-단백질의 상호작용에 관한 연구", Restorative Dentistry and Endodontics, 32(5), pp.459~468 (2007), 대한치과보존학회
- Aberg T, "Expression patterns of bone morphogenetic proteins(Bmps)in the developing mouse tooth suggest roles in morphogenesis and cell differentiation", Dev Dyn, 210, pp.383~396 (1997)
- Jiang FX, "Convergence of bone morphogenetic protein and laminin-1 signaling pathways promotes proliferation and colony formation by fetal mouse pancreatic cells", Exp Cell Res, 308, pp.114~122 (2005)
- Miyazono K, "BMP receptor signaling:transcriptional targets,regulation of signals,and signaling cross-talk", Cytokine Growth Factor Rev, 16, pp.251~263 (2005)
- 박종태, "법랑모세포 분화와 법랑질 형성과정에서 OD314, Apin protein의 발현 및 기능", Restorative Dentistry and Endodontics, 31(6), pp.437~444 (2006), 대한치과보존학회
- 김흥중, "RNA interference를 이용한 OD314 유전자의 발현억제가 상아질모세포 전구세포에 미치는 영향", 대한체질인류학회지, 17(2), pp.121~129 (2004), 대한체질인류학회
- Yasuhara R, "Nitric oxide in pulp cell growth,differentiation,and mineralization", J Dent Res, 86, pp.163~168 (2007)
- Buchaille R, "A substractive PCR-based cDNA library from human odontoblast cells:identification of novel genes expressed in tooth forming cells", Matrix Biol, 19, pp.421~430 (2000)
- Wistuba J, "Tooth development in Ambystoma mexicanum:phosphatase activities,calcium accumulation and cell proliferation in the tooth-forming tissues", Ann Anat, 185, pp.239~245 (2003)
- Woltgens JH, "Biomineralization during early stages of the developing tooth in vitro with special reference to secretory stage of amelogenesis", Int J Dev Biol, 39, pp.203~212 (1995)
- Nakata A, "Establishment and characterization of a spontaneously immortalized mouse ameloblast-lineage cell line", Biochem Biophys Res Commun, 308, pp.834~839 (2003)
- Wise GE, "Changes in the tartrate-resistant acid phosphatase cell population in dental follicles and bony crypts of rat molars during tooth eruption", J Dent Res, 68(2), pp.150~156 (1989)
- Deutsch D, "Tuftelin:enamel mineralization and amelogenesis imperfecta", Ciba Found Symp, 205, pp.135~147 (1997)
- Shi Y, "Mechanisms of TGF-beta signaling from cell membrane to the nucleus", Cell, 113, pp.685~700 (2003)
- Chen J, "TheTGF-beta pseudoreceptor gene Bambi is dispensable for mouse embryonic development and postnatal survival", Genesis, 45(8), pp.482~486 (2007)
- Iohara K, "Dentin regeneration by dental pulp stem cell therapy with recombinant human bone morphogenetic protein 2", J Dent Res, 83, pp.590~595 (2004)
- Nadiri A, "Expression patterns of BMPRs in the developing mouse molar", Cell Tissue Res, 324, pp.33~40 (2006)
- Kestler DP, "Expression of Odontogenic Ameloblast-Associated Protein(ODAM)in Dental and Other Epithelial Neoplasms", Mol Med, 14, pp.318~326 (2008)