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山藥의 항산화 작용에 대한 단백질체 분석 연구

Proteomic Approach to Study the Antioxidant Activities of Dioscoreae Rhizoma on HeLa Cells

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Abstract

Purpose: This study was examined to verify the anti-oxidative effect of Dioscoreae Rhizoma on HeLa cells by proteomic approach. Methods: Aqueous extract was used to treat HeLa cell with different concentrations treated with water or MeOH extract of Dioscoreae Rhizoma. HeLa cells were co-treated with H2O2 and Dioscoreae Rhizoma extracts. Proteomics was done to identify, characterize, and quantitate proteins expressed in HeLa cells treated by H2O2 and Dioscoreae Rhizoma. Results: When HeLa cells were Co-treated with H2O2 and Dioscoreae batatas extracts, 16 proteins identified by 2-DE and MALDI-TOF mass spectrometry and database search. PRDX, HSP27 was major proteins of antioxidant effect by Dioscoreae batatas. Conclusion: Our results suggest that Dioscoreae Rhizoma extracts induce antioxidant effects by regulating proteins such as PRDX, HSP27.

참고문헌 (59)

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