흰 쥐의 측두하악관절 통증모델에서 Triptolide와 N-nitro-L-arginine Methyl Ester의 통증조절효과
Analgesic Effects of Triptolide and N-nitro-L-arginine Methyl Ester in Rat’s Temporomandibular Joint Pain Model
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이 연구는 triptolide와 L-NAME의 측두하악관절 통증조절효과를 확인하기 위하여 포르말린으로 유도된 측두하악관절 통증모델에서 triptolide와 L-NAME의 소뇌연수조 내각각의 얄물의 단독 투여에 따른 통증행위반응과 두 약물의병용 투여에 따른 상호작용이 통증행위반응에 미치는 영향을 평가하여 다음과 같은 결과를 얻었다. 먼저, 관절강 내로주입한 5% 포르말린(30 μl)은 유의한 통증행위반응을 유발하였고, 2차 통증행위반응 관찰 시 포르말린 주입 전 1 μg/ 10 μl triptolide 투여군(163.33±29.11회)은 포르말린군(308± 33.04회)과 비교 시 통증행위반응이 유의하게 감소하였다. 0.1 μg/10 μl의 L-NAME 투여군의 1, 2차 통증행위반응의결과, 각각 5.80±3.75회, 92.30±16.04회로 포르말린 주입군 25.4±6.59회, 285.60±29.93회와 비교 시 유의하게 감소되었다. 다음으로, 1 μg/10 μl의 triptolide와 0.01 μg/10 μl 의 L-NAME 병용 투여군에서 1, 2차 통증행위반응이 0.80± 0.80회, 96.50±26.16회로 나타나 22.50±19.15회, 163.33± 29.11회로 나타난 1 μg/10 μl trtiptolide군과 비교 시 유의하게 통증행위반응이 경감되었다. 이러한 연구결과는 측두하악관절 통증조절의 예방 및 치료에 있어 활용가능한 천연물로 triptolide가 제시될 수 있으며, 천연물과 화합물들의 병용 투여를 통해 그 효과를 증가시킬 수 있을 것으로 기대된다.
Abstract
The aim of this study was to investigate whether intracisternal administrations of triptolide and N-nitro-L-arginine Methyl Ester (L-NAME) are involved in the regulation of temporomandibular joint (TMJ) pain. The TMJ pain was induced by the injection of 5% formalin (30 μl) into TMJ capsule of rats. The pain behavioral responses was recorded the number of grooming or scratching on the left TMJ area for 9 successive 5 minutes intervals. Triptolide and L-NAME were administrated intracisternally 10 minutes before formalin injection. The intra-articular injection of formalin produced a biphasic pattern of pain response (first phase: 0∼10 minutes and second phase: 11∼45 minutes). The intracisternal administration of triptolide (1 μg/10 μl) and L-NAME (0.1 μg/10 μl) suppressed the TMJ pain behavior in each experiment. Co-administration of two drugs was shown the enhanced effect than the analgesic effect by single-administration of triptolide (1 μg/10 μl). The triptolide could be a useful analgesic agent for the treatment of TMJ pain, and it is expected to reduce the substantial amount of it via co-administration of synthetic chemical compound and natural products.
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