Effect of Combination Electroacupuncture and Tenuigenin on the Migration and Differentiation of Mesenchymal Stem Cells following Ischemic Stroke
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Objectives: Since stroke is a serious health issue, novel therapeutic strategies are required. In a mouse model of ischemic stroke, this study analyzed the potential of electroacupuncture (EA) and tenuigenin (TE) to improve the efficacy of human mesenchymal stem cell (hMSC) transplantation. Methods: Middle cerebral artery occlusion (MCAO) with reperfusion was used to generate ischemic stroke. Forty-eight male C57BL/6 mice were randomly divided into five groups: control, MCAO-operated, MCAO-EA, MCAO-TE, or MCAO + EA + TE. Subsequently, hMSCs were transplanted into the ischemic region and EA, TE, or the combination was administered. Behavior assessments and immunohistochemistry were conducted to evaluate motor and cognitive recovery and hMSCs survival, migration, and differentiation. Results: The combined treatment of EA and TE exhibited enhanced hMSCs survival, migration and differentiation into neural cell lineages while suppressing astrocyte formation. Immunohistochemistry demonstrated increased neurogenesis through hMSCs transplantation in the ischemic brain. Immediate behavioral improvements were not significantly different between groups, but there was a gradual recovery in motor and cognitive function over time. Conclusion: These findings highlight the potential of EA and TE co-treatment as a therapeutic strategy for ischemic stroke, opening avenues for further research to optimize treatment protocols and elucidate underlying mechanisms.
Abstract
Objectives: Since stroke is a serious health issue, novel therapeutic strategies are required. In a mouse model of ischemic stroke, this study analyzed the potential of electroacupuncture (EA) and tenuigenin (TE) to improve the efficacy of human mesenchymal stem cell (hMSC) transplantation. Methods: Middle cerebral artery occlusion (MCAO) with reperfusion was used to generate ischemic stroke. Forty-eight male C57BL/6 mice were randomly divided into five groups: control, MCAO-operated, MCAO-EA, MCAO-TE, or MCAO + EA + TE. Subsequently, hMSCs were transplanted into the ischemic region and EA, TE, or the combination was administered. Behavior assessments and immunohistochemistry were conducted to evaluate motor and cognitive recovery and hMSCs survival, migration, and differentiation. Results: The combined treatment of EA and TE exhibited enhanced hMSCs survival, migration and differentiation into neural cell lineages while suppressing astrocyte formation. Immunohistochemistry demonstrated increased neurogenesis through hMSCs transplantation in the ischemic brain. Immediate behavioral improvements were not significantly different between groups, but there was a gradual recovery in motor and cognitive function over time. Conclusion: These findings highlight the potential of EA and TE co-treatment as a therapeutic strategy for ischemic stroke, opening avenues for further research to optimize treatment protocols and elucidate underlying mechanisms.
참고문헌 (23)
- "World stroke day" (2021)[URL]
- Powers WJ, "2018 Guidelines for the early management of patients with acute ischemic stroke: a guideline for healthcare professionals from the American Heart Association", American Stroke Association, 2018, pp.49(3), e46~e110
- Pittenger MF, "Multilineage potential of adult human mesenchymal stem cells", Science, 284(5411), pp.143~147 (1999)
- Zhang Y, "Ferroptosis and its multifaceted roles in cerebral stroke", Front Cell Neurosci, 15, pp.615372~ (2021)
- Shin HK, "Modulation of neurogenesis via neurotrophic factors in acupuncture treatments for neurological diseases", Biochem Pharmacol, 141, pp.132~142 (2017)
- Xing Y, "Mechanisms involved in the neuroprotection of electroacupuncture therapy for ischemic stroke", Front Neurosci, 12, pp.929~ (2018)
- Kim JH, "Electroacupuncture preconditioning reduces cerebral ischemic injury via BDNF and SDF-1α in mice", BMC Complement Altern Med, 13, pp.22~ (2013)
- Kim YR, "Electroacupuncture promotes post-stroke functional recovery via enhancing endogenous neurogenesis in mouse focal cerebral ischemia", PLoS One, 9(2), pp.e90000~ (2014)
- Lee HJ, "Effects of electroacupuncture on the functionality of NG2-expressing cells in perilesional brain tissue of mice following ischemic stroke", Neural Regen Res, 17(7), pp.1556~1565 (2022)
- Lee HJ, "Positive effects of α-asarone on transplanted neural progenitor cells in a murine model of ischemic stroke", Phytomedicine, 51, pp.151~161 (2018)
- Chen YJ, "Tenuigenin protects cultured hippocampal neurons against methylglyoxalinduced neurotoxicity", Eur J Pharmacol, 645(1-3), pp.1~8 (2010)
- Chen Y, "Tenuigenin promotes proliferation and differentiation of hippocampal neural stem cells", Neurochem Res, 37(4), pp.771~777 (2012)
- Ahn SM, "Therapeutic potential of a combination of electroacupuncture and TrkB-expressing mesenchymal stem cells for ischemic stroke", Mol Neurobiol, 56(1), pp.157~173 (2019)
- Andrzejewska A, "Mesenchymal stem cells for neurological disorders", Adv Sci(Weinh), 8(7), pp.2002944~ (2021)
- Kim YR, "Potential benefits of mesenchymal stem cells and electroacupuncture on the trophic factors associated with neurogenesis in mice with ischemic stroke", Sci Rep, 8(1), pp.2044~ (2018)
- Cheng CY, "Electroacupuncture-like stimulation at Baihui and Dazhui acupoints exerts neuroprotective effects through activation of the brain-derived neurotrophic factor-mediated MEK1", 2, 2, pp.p90RSK, bad signaling pathway in mild transient focal cerebral ischemia in rats[DOI]
- Tao J, "Electroacupuncture at LI11 and ST36 acupoints exerts neuroprotective effects via reactive astrocyte proliferation after ischemia and reperfusion injury in rats", Brain Res Bull, 120, pp.14~24 (2016)
- Fan Z, "Tenuigenin protects dopaminergic neurons from inflammation via suppressing NLRP3 inflammasome activation in microglia", J Neuroinflammation, 14(1), pp.256~ (2017)
- Wang X, "Tenuigenin inhibits LPS-induced inflammatory responses in microglia via activating the Nrf2-mediated HO-1 signaling pathway", Eur J Pharmacol, 809, pp.196~202 (2017)
- Huang JN, "Tenuigenin treatment improves behavioral Y-maze learning by enhancing synaptic plasticity in mice", Behav Brain Res, 246, pp.111~115 (2013)
- Salgado AJ, "Mesenchymal stem cells secretome as a modulator of the neurogenic niche : basic insights and therapeutic opportunities", Front Cell Neurosci, 9, pp.249~ (2015)
- Rahman AA, "Neurogenesis after stroke : a therapeutic perspective", Transl Stroke Res, 12(1), pp.1~14 (2021)
- Shen XY, "Activation and role of astrocytes in ischemic stroke", Front Cell Neurosci, 15, pp.755955~ (2021)