Hydroalcoholic Extract of Scrophularia Striata Attenuates Hypertrophic Scar, Suppresses Collagen Synthesis, and Stimulates MMP2 and 9 Gene Expression in Rabbit Ear Model
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Objectives: Hypertrophic scars (HSs) are caused by abnormal wound healing. To date, no standard treatment has been made available for HSs. Scrophularia striata has been reported to accelerate wound healing and has the potential to prevent HS formation. In this study, we investigated the anti-scarring effects of S. striata extract (SSE) in a rabbit ear model of scarring. Methods: In this study, New Zealand white rabbit (weight: 2.3-2.5 kg) were used. In the prevention phase of the study, three test groups received 5%, 10%, and 15% ointments of SSE in the Eucerin base, the fourth group received Eucerin, and the fifth group received no treatment. The samples were obtained on day 35 after wounding. In the treatment phase of the study, the test groups received an intralesional injection of SSE (5%, 10%, and 15%), the fourth group received an intralesional injection of triamcinolone, the fifth group received a solvent (injection vehicle), and the sixth group received no treatment. To evaluate the anti-scarring effects of SSE, the scar elevation index (SEI), epidermis thickness index (ETI), collagen deposition, and MMP2 and MMP9 gene expression were evaluated. Results: A significant reduction in SEI, ETI, and collagen deposition was noted in animals treated with SSE compared with the control groups. In addition, topical SSE stimulated MMP2 and MMP9 gene expression. Conclusion: The findings of this study demonstrate the potential for SSE in the prevention and treatment of HS. SSE could be prepared as an appropriate formulation to treat wounds and prevent abnormal scarring.
Abstract
Objectives: Hypertrophic scars (HSs) are caused by abnormal wound healing. To date, no standard treatment has been made available for HSs. Scrophularia striata has been reported to accelerate wound healing and has the potential to prevent HS formation. In this study, we investigated the anti-scarring effects of S. striata extract (SSE) in a rabbit ear model of scarring. Methods: In this study, New Zealand white rabbit (weight: 2.3-2.5 kg) were used. In the prevention phase of the study, three test groups received 5%, 10%, and 15% ointments of SSE in the Eucerin base, the fourth group received Eucerin, and the fifth group received no treatment. The samples were obtained on day 35 after wounding. In the treatment phase of the study, the test groups received an intralesional injection of SSE (5%, 10%, and 15%), the fourth group received an intralesional injection of triamcinolone, the fifth group received a solvent (injection vehicle), and the sixth group received no treatment. To evaluate the anti-scarring effects of SSE, the scar elevation index (SEI), epidermis thickness index (ETI), collagen deposition, and MMP2 and MMP9 gene expression were evaluated. Results: A significant reduction in SEI, ETI, and collagen deposition was noted in animals treated with SSE compared with the control groups. In addition, topical SSE stimulated MMP2 and MMP9 gene expression. Conclusion: The findings of this study demonstrate the potential for SSE in the prevention and treatment of HS. SSE could be prepared as an appropriate formulation to treat wounds and prevent abnormal scarring.
참고문헌 (29)
- van der Veer WM, "Potential cellular and molecular causes of hypertrophic scar formation", Burns, 35(1), pp.15~29 (2009)
- Mokos ZB, "Current therapeutic approach to hypertrophic scars", Front Med (Lausanne), 4, pp.83~ (2017)
- English RS, "Keloids and hypertrophic scars", Dermatol Surg, 25(8), pp.631~638 (1999)
- Zhu Z, "The molecular mechanism of hypertrophic scar", J Cell Commun Signal, 7(4), pp.239~252 (2013)
- Rohani MG, "Matrix remodeling by MMPs during wound repair", Matrix Biol, 44-46, pp.113~121 (2015)
- Sawicki G, "Interaction of keratinocytes and fibroblasts modulates the expression of matrix metalloproteinases-2 and-9 and their inhibitors", Mol Cell Biochem, 269(1-2), pp.209~216 (2005)
- Zhu Z, "The molecular basis of hypertrophic scars", Burns Trauma, 4, pp.2~ (2016)
- Ulrich D, "TIMP-1, MMP-2, MMP-9, and PIIINP as serum markers for skin fibrosis in patients following severe burn trauma", Plast Reconstr Surg, 111(4), pp.1423~1431 (2003)
- Ye Q, "Medicinal plants for the treatment of hypertrophic scars", Evid Based Complement Alternat Med, 2015, pp.101340~ (2015)
- Ghashghaii A, "Wound healing potential of Methanolic extract of Scrophularia striata in rats", Pharm Sci, 23(4), pp.256~263 (2017)
- Tanideh N, "The healing effect of scrophularia striata on experimental burn wounds infected to pseudomonas aeruginosa in rat", World J Plast Surg, 4(1), pp.16~23 (2015)
- Monsef-Esfahani HR, "Flavonoids, cinnamic acid and phenyl propanoid from aerial parts of Scrophularia striata", Pharm Biol, 48(3), pp.333~336 (2010)
- Tamri P, "A mini-review on phytochemistry and pharmacological activities of Scrophularia striata", J Herbmed Pharmacol, 8(2), pp.85~89 (2019)
- SharifiN, "Effect of Scrophularia striata plant on episiotomy wound healing in primiparous women", Iran J Obstet Gynecol Infertil, 20(1), pp.56~60 (2017)
- Tamri P, "Modulation of extracellular matrix by Scrophularia striata extract in vitro : a potential antiscarring agent", Jundishapur J Nat Pharm Prod, 15(3), pp.e95301~ (2020)
- Morris DE, "Acute and chronic animal models for excessive dermal scarring : quantitative studies", Plast Reconstr Surg, 100(3), pp.674~681 (1997)
- Feng Y, "Targeted apoptosis of myofibroblasts by elesclomol inhibits hypertrophic scar formation", EBioMedicine, 54, pp.102715~ (2020)
- Shi HX, "The anti-scar effects of basic fibroblast growth factor on the wound repair in vitro and in vivo", PLoS One, 8(4), pp.e59966~ (2013)
- Cheng L, "In vivo early intervention and the therapeutic effects of 20(s)-ginsenoside rg3on hypertrophic scar formation", PLoS One, 9(12), pp.e113640~ (2014)
- Chen Y, "A convenient method for quantifying collagen fibers in atherosclerotic lesions by ImageJ software", Int J Clin Exp Med, 10(10), pp.14904~14910 (2017)
- Haddadi R, "In vitro wound healing activity of Scrophularia striata hydroalcoholic extract", S Afr J Bot, 121, pp.505~509 (2019)
- Kloeters O, "Hypertrophic scar model in the rabbit ear : a reproducible model for studying scar tissue behavior with new observations on silicone gel sheeting for scar reduction", Wound Repair Regen, 1, pp.S40~S45 (2007)
- Sanon S, "Skin tissue engineering and regenerative medicine", pp.19~47 (2016), Academic Press
- Azadmehr A, "Antioxidant and neuroprotective effects of Scrophularia striata extract against oxidative stress-induced neurotoxicity", Cell Mol Neurobiol, 33(8), pp.1135~1141 (2013)
- Azadmehr A, "Protective effects of Scrophularia striata in Ovalbumin-induced mice asthma model", Daru, 21(1), pp.56~ (2013)
- Dang CM, "Scarless fetal wounds are associated with an increased matrix metalloproteinase-to-tissue-derived inhibitor of metalloproteinase ratio", Plast Reconstr Surg, 111(7), pp.2273~2285 (2003)
- Manuel JA, "Matrix metalloproteinase 9(MMP-9) is upregulated during scarless wound healing in athymic nude mice", Matrix Biol, 25(8), pp.505~514 (2006)
- Xue M, "Extracellular matrix reorganization during wound healing and its impact on Abnormal scarring", Adv Wound Care(New Rochelle), 4(3), pp.119~136 (2015)
- Asimakidou M, "Regulation of matrix metalloproteinase-2and-9 during healing of dermal wounds after incision using radiofrequency energy in neonatal and adult rats", Hippokratia, 21(2), pp.85~92 (2017)