Anxiolytic and Antianhedonic-like Effects of Psidium guajava Leaf in Alcohol-Withdrawn Mice
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Objectives: Alcohol withdrawal syndrome manifests through a range of symptoms, including anxiety and anhedonia, significantly affecting the quality of life of those affected. This study investigates the potential therapeutic effects of the methanolic extract of Psidium guajava leaves (MPG) on anxiety and anhedonia in Swiss albino female mice undergoing alcohol withdrawal. Methods: Four groups of mice underwent alcohol withdrawal, with one group undergoing saline withdrawal as a control. On the test day, behavioral assessments were conducted to evaluate anxiety and anhedonia. Groups I and II received sodium carboxymethylcellulose, Group III received diazepam, and Groups IV and V received varying oral doses of MPG. Results: The results indicate significant anti-anhedonic and anxiolytic effects of MPG. These effects were observed through changes in parameters measured in the Open Field test, Elevated Plus Maze test, Marble Burying test, and Sucrose Preference test. Mice treated with MPG displayed reduced anxiety-like behaviors and increased sucrose preference compared to untreated mice undergoing alcohol withdrawal. Conclusion: These findings suggest that Psidium guajava leaf extract may have therapeutic potential in alleviating anxiety and anhedonia associated with alcohol withdrawal. The observed effects indicate that MPG could serve as a promising adjunct therapy for managing alcohol withdrawal symptoms, thereby enhancing the overall well-being of individuals undergoing alcohol cessation.
Abstract
Objectives: Alcohol withdrawal syndrome manifests through a range of symptoms, including anxiety and anhedonia, significantly affecting the quality of life of those affected. This study investigates the potential therapeutic effects of the methanolic extract of Psidium guajava leaves (MPG) on anxiety and anhedonia in Swiss albino female mice undergoing alcohol withdrawal. Methods: Four groups of mice underwent alcohol withdrawal, with one group undergoing saline withdrawal as a control. On the test day, behavioral assessments were conducted to evaluate anxiety and anhedonia. Groups I and II received sodium carboxymethylcellulose, Group III received diazepam, and Groups IV and V received varying oral doses of MPG. Results: The results indicate significant anti-anhedonic and anxiolytic effects of MPG. These effects were observed through changes in parameters measured in the Open Field test, Elevated Plus Maze test, Marble Burying test, and Sucrose Preference test. Mice treated with MPG displayed reduced anxiety-like behaviors and increased sucrose preference compared to untreated mice undergoing alcohol withdrawal. Conclusion: These findings suggest that Psidium guajava leaf extract may have therapeutic potential in alleviating anxiety and anhedonia associated with alcohol withdrawal. The observed effects indicate that MPG could serve as a promising adjunct therapy for managing alcohol withdrawal symptoms, thereby enhancing the overall well-being of individuals undergoing alcohol cessation.
참고문헌 (21)
- Pandy V, "Protective effect of methanolic extract of Areca catechu nut on ethanol withdrawal symptoms in mice", Futur J Pharm Sci, 9, pp.10~ (2023)
- "Alcohol"[URL]
- Mirijello A, "Identification and management of alcohol withdrawal syndrome", Drugs, 75(4), pp.353~365 (2015)
- Gupta A, "Novel targets explored in the treatment of alcohol withdrawal syndrome", CNS Neurol Disord Drug Targets, 20(2), pp.158~173 (2021)
- Kumar M, "Guava(Psidium guajava L. )leaves : nutritional composition, phytochemical profile, and health-promoting bioactivities", Foods, 10(4), pp.752~ (2021)
- Devi VH, "A review on phytochemical, biological, and pharma-cological effects of Psidium guajava Linn. (Guava). Int J Pharm Sci", Nanotech, 17(1), pp.7173~7182 (2024)
- Arya V, "Preliminary phytochemical analysis of the extracts of Psidium leaves", J Pharmacogn Phytochem, 1(1), pp.1~5 (2012)
- Seibenhener ML, "Use of the Open Field Maze to measure locomotor and anxiety-like behavior in mice", J Vis Exp, 96, pp.e52434~ (2015)
- Narasingam M, "Anxiolytic-and antidepressant-like activities of a methanolic extract of Morinda citrifolia Linn. (noni)fruit in mice : involvement of benzodiazepine-GABAAergic, serotonergic and adrenergic systems", Biomed Pharmacother, 96, pp.944~952 (2017)
- Kedia S, "Marble burying as a test of the delayed anxiogenic effects of acute immobilisation stress in mice", J Neurosci Methods, 233, pp.150~154 (2014)
- Farook JM, "Acamprosate attenuates the handling induced convulsions during alcohol withdrawal in Swiss Webster mice", Physiol Behav, 95(1-2), pp.267~270 (2008)
- Becker HC, "Neurochemical mechanisms of alcohol withdrawal", Handb Clin Neurol, 125, pp.133~156 (2014)
- Lovinger DM, "Serotonin’s role in alcohol’s effects on the brain", Alcohol Health Res World, 21(2), pp.114~120 (1997)
- Fu R, "Anxiety during alcohol withdrawal involves 5-HT2C receptors and M-channels in the lateral habenula", Neuropharmacology, 163, pp.107863~ (2020)
- Tachakittirungrod S, "Study on antioxidant activity of certain plants in Thailand : mechanism of antioxidant action of guava leaf extract", Food Chem, 103(2), pp.381~388 (2007)
- Sultana B, "Effect of extraction solvent", technique on the antioxidant activity of selected medicinal plant extracts, 2009, pp.14(6), 2167~2180
- Biswas S, "Evaluation of neurobehavioral activities of ethanolic extract of Psidium guajava Linn leaves in mice model", Futur J Pharm Sci, 7(1), pp.36~ (2021)
- Ruksiriwanich W, "Guava(Psidium guajava L. )leaf extract as bioactive substances for anti-androgen and antioxidant activities", Plants(Basel), 11(24), pp.3514~ (2022)
- Hernandez-Leon A, "The anxiolytic-like effect of rutin in rats involves GABAA receptors in the basolateral amygdala", Behav Pharmacol, 28(4), pp.303~312 (2017)
- Islam MS, "Anxiolytic-like effect of quercetin possibly through GABA receptor interaction pathway : in vivo and in silico studies", Molecules, 27(21), pp.7149~ (2022)
- Grundmann O, "Kaempferol from the leaves of Apocynum venetum possesses anxiolytic activities in the elevated plus maze test in mice", Phytomedicine, 16(4), pp.295~302 (2009)