The effect of treadmill exercise on axonal regrowth and muscle hypertrophy via activation of the Akt/mTOR signaling pathway after sciatic nerve injury
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The aim of this study was to investigate whether treadmill exercise may increase neurite outgrowth after which may affect Akt/mTOR signaling pathway for inhibition of muscle atrophy after SNI. The experimental animals were divided randomly into 3 group of control group (n=10), exercise group (n=10) for 7 days/ (n=10) for 14 days, and sedentary group (n=10) for 7 days/ (n=10) for 14 days respectively. Western blot analysis and Immunofluorescence staining were used to analyze for this study. The result of this study was that treadmill exercise influenced positively on neurite outgrowth required in axonal regeneration by facilitation of GAP-43 after SNI. Then treadmill exercise facilitated Akt and induced upward expression of p-mTOR and p-4E-BP1, thus influenced on protein synthesis and muscle atrophy significantly. Consequently, treadmill exercise facilitated axonal regeneration and inhibited muscle atrophy through biochemical modification of Akt/mTOR pathway at target muscle. Therefore physical activities inducing muscle contraction to facilitate an axonal regeneration at injured nerve may be important strategy for rehabilitation.
Abstract
The aim of this study was to investigate whether treadmill exercise may increase neurite outgrowth after which may affect Akt/mTOR signaling pathway for inhibition of muscle atrophy after SNI. The experimental animals were divided randomly into 3 group of control group (n=10), exercise group (n=10) for 7 days/ (n=10) for 14 days, and sedentary group (n=10) for 7 days/ (n=10) for 14 days respectively. Western blot analysis and Immunofluorescence staining were used to analyze for this study. The result of this study was that treadmill exercise influenced positively on neurite outgrowth required in axonal regeneration by facilitation of GAP-43 after SNI. Then treadmill exercise facilitated Akt and induced upward expression of p-mTOR and p-4E-BP1, thus influenced on protein synthesis and muscle atrophy significantly. Consequently, treadmill exercise facilitated axonal regeneration and inhibited muscle atrophy through biochemical modification of Akt/mTOR pathway at target muscle. Therefore physical activities inducing muscle contraction to facilitate an axonal regeneration at injured nerve may be important strategy for rehabilitation.
참고문헌 (20)
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