Clinicopathologic significance of the expression of Snail in hepatocellular carcinoma
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Background/Aims: E-cadherin is involved in intercellular binding and cellular polarity formation. Snail is a key regulator of the epithelial-mesenchymal transition and is closely associated with tumor invasiveness due to its ability to suppress E-cadherin expression. We investigated the expressions of E-cadherin and Snail in hepatocellular carcinoma (HCC) tissue to determine the clinical significance of these proteins in HCC. Methods: Immunohistochemistry was used to examine the expressions of E-cadherin and Snail in resected tissues from 59 patients diagnosed with HCC. We also evaluated the relationship between the expressions of these two molecules in HCC tissue and clinicopathologic factors in the patients. Results: Immunohistochemistry showed that Snail was stained in 20.3% of the HCC tissues and 3.4% of noncancerous tissues. Snail was not stained in the area of E-cadherin expression. The expression of Snail in the HCC tissue was associated with poorly differentiated HCC (P=0.028). The expression of Snail without E-cadherin staining in HCC tissue was significantly associated with postoperative HCC recurrence (P=0.013). Conclusions: The expression of Snail in HCC tissue was associated with decreased expression of E-cadherin and poorly differentiated HCC. The expression of Snail without E-cadherin staining in HCC was associated with postoperative recurrence.
Abstract
Background/Aims: E-cadherin is involved in intercellular binding and cellular polarity formation. Snail is a key regulator of the epithelial-mesenchymal transition and is closely associated with tumor invasiveness due to its ability to suppress E-cadherin expression. We investigated the expressions of E-cadherin and Snail in hepatocellular carcinoma (HCC) tissue to determine the clinical significance of these proteins in HCC. Methods: Immunohistochemistry was used to examine the expressions of E-cadherin and Snail in resected tissues from 59 patients diagnosed with HCC. We also evaluated the relationship between the expressions of these two molecules in HCC tissue and clinicopathologic factors in the patients. Results: Immunohistochemistry showed that Snail was stained in 20.3% of the HCC tissues and 3.4% of noncancerous tissues. Snail was not stained in the area of E-cadherin expression. The expression of Snail in the HCC tissue was associated with poorly differentiated HCC (P=0.028). The expression of Snail without E-cadherin staining in HCC tissue was significantly associated with postoperative HCC recurrence (P=0.013). Conclusions: The expression of Snail in HCC tissue was associated with decreased expression of E-cadherin and poorly differentiated HCC. The expression of Snail without E-cadherin staining in HCC was associated with postoperative recurrence.
참고문헌 (30)
- Hai-Rim Shin, "2002 Annual Report of the Korea Central Cancer Registry: Based on Registered Data from 139 Hospitals", Cancer Research and Treatment, 36(2), pp.103~114 (2004), 대한암학회
- Tung-Ping Poon R, "Risk factors, prevention, and management of postoperative recurrence after resection of hepatocellular carcinoma", Ann Surg, 232, pp.10~24 (2000)
- Takenaka K, "Results of 280 liver resections for hepatocellular carcinoma", Arch Surg, 131, pp.71~76 (1996)
- Lise M, "Prognostic factors affecting long term outcome after liver resection for hepatocellular carcinoma: results in a series of 100 Italian patients", Cancer, 82, pp.1028~1036 (1998)
- Okuda K, "Natural history of hepatocellular carcinoma and prognosis in relation to treatment. Study of 850 patients", Cancer, 56, pp.918~928 (1985)
- Fan ST, "Hepatectomy for hepatocellular carcinoma: toward zero hospital deaths", Ann Surg, 229, pp.322~330 (1999)
- Fong Y, "An analysis of 412 cases of hepatocellular carcinoma at a Western center", Ann Surg, 229, pp.790~799 (1999)
- Vauthey JN, "Factors affecting long-term outcome after hepatic resection for hepatocellular carcinoma", Am J Surg, 169, pp.28~34 (1995)
- Shimada M, "Risk factors of the recurrence of hepatocellular carcinoma originating from residual cancer cells after hepatectomy", Hepatogastroenterology, 46, pp.2469~2475 (1999)
- Adachi E, "Factors correlated with portal venous invasion by hepatocellular carcinoma: univariate and multivariate analyses of 232 resected cases without preoperative treatments", Cancer, 77, pp.2022~2031 (1996)
- Gotzmann J, "Hepatocytes convert to a fibroblastoid phenotype through the cooperation of TGF-beta1 and Ha-Ras: steps towards invasiveness", J Cell Sci, 115, pp.1189~1202 (2002)
- Grau Y, "Mutations and Chromosomal Rearrangements Affecting the Expression of Snail, a Gene Involved in Embryonic Patterning in DROSOPHILA MELANOGASTER", Genetics, 108, pp.347~360 (1984)
- Cano A, "The transcription factor snail controls epithelial-mesenchymal transitions by repressing E-cadherin expression", Nat Cell Biol, 2, pp.76~83 (2000)
- Olmeda D, "Snail silencing effectively suppresses tumour growth and invasiveness", Oncogene, 26, pp.1862~1874 (2007)
- Miyoshi A, "Snail accelerates cancer invasion by upregulating MMP expression and is associated with poor prognosis of hepatocellular carcinoma", Br J Cancer, 92, pp.252~258 (2005)
- Blanco MJ, "Correlation of Snail expression with histological grade and lymph node status in breast carcinomas", Oncogene, 21, pp.3241~3246 (2002)
- Sugimachi K, "Transcriptional repressor snail and progression of human hepatocellular carcinoma", Clin Cancer Res, 9, pp.2657~2664 (2003)
- Ueno S, "Prognostic performance of the new classification of primary liver cancer of Japan (4th edition) for patients with hepatocellular carcinoma: a validation analysis", Hepatol Res, 24, pp.395~403 (2002)
- Edmondson HA, "Primary carcinoma of the liver: a study of 100 cases among 48,900 necropsies", Cancer, 7, pp.462~503 (1954)
- Takeichi M, "Cadherin cell adhesion receptors as a morphogenetic regulator", Science, 251, pp.1451~1455 (1991)
- Endo K, "Immunoreactive E-cadherin, alpha-catenin, beta-catenin, and gamma-catenin proteins in hepatocellular carcinoma: relationships with tumor grade, clinicopathologic parameters, and patients' survival", Hum Pathol, 31, pp.558~565 (2000)
- Leptin M, "Cell shape changes during gastrulation in Drosophila", Development, 110, pp.73~84 (1990)
- Jiao W, "Inverse correlation between E-cadherin and Snail expression in hepatocellular carcinoma cell lines in vitro and in vivo", Br J Cancer, 86, pp.98~101 (2002)
- Bae SH, "Expression patterns of E-cadherin and beta-catenin according to clinicopathological characteristics of hepatocellular carcinoma", Taehan Kan Hakhoe Chi, 8, pp.297~303 (2002)
- Batlle E, "The transcription factor snail is a repressor of E-cadherin gene expression in epithelial tumour cells", Nat Cell Biol, 2, pp.84~89 (2000)
- Yokoyama K, "Reverse correlation of E-cadherin and snail expression in oral squamous cell carcinoma cells in vitro", Oral Oncol, 37, pp.65~71 (2001)
- Ihara A, "Expression of epithelial cadherin and alpha- and beta-catenins in nontumoral livers and hepatocellular carcinomas", Hepatology, 23, pp.1441~1447 (1996)
- Wei Y, "Altered expression of E-cadherin in hepatocellular carcinoma: correlations with genetic alterations, beta-catenin expression, and clinical features", Hepatology, 36, pp.692~701 (2002)
- Matsumura T, "Frequent down-regulation of E-cadherin by genetic and epigenetic changes in the malignant progression of hepatocellular carcinomas", Clin Cancer Res, 7, pp.594~599 (2001)
- Kanai Y, "The E-cadherin gene is silenced by CpG methylation in human hepatocellular carcinomas", Int J Cancer, 71, pp.355~359 (1997)