추출조건에 따른 陳橘 및 升麻 추출물의 미백 활성효과
Inhibitory effect of Citrus sunki and Cimicifuga dahurica extracts on melanogenesis accroding to the various extraction methods
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Objectives: Excessive melanin production leads to skin pigmentation, which causes various cosmetic and health problems. Citrus unshiu Markovich peel (CS) and Cimicifuga dahurica (CD) have long been widely used as a oriental medicinal plant because of their pharmacological properties including anti-ulcer, anti-oxidant, and anti-inflammatory properties. Methods: In this study, we investigated the inhibitory effects of CS, CD extracts or CS:CD=1:2 mixture on melanogenesis according to the various extraction methods. CS and CD extracted were prepared by ethanol extraction (EE), ultrasonification extraction (USE), Supercritical extraction (SCE), reflux extraction (RE), respectively. Results: DPPH radical scavenging and tyrosinase inhibitory activity of CD extracts or CS:CD=1:2 mixture were increased in dose-dependent manners. In addition, we evaluated the effect of CS, CD extracts or CS:CD=1:2 mixture on tyrosinase activity and melanogenesis in α-melanocyte stimulating hormone-induced B16-F10 melanoma cells. CS, CD extracts or CS:CD=1:2 mixture significantly inhibited tyrosinase activity and melanogenesis at 10-200 μg/mL. Conclusions: Therefore, our study suggests that CS and CD extracts have potential as a safe treatment for excessive pigmentation or as a natural ingredient in cosmetics.
Abstract
Objectives: Excessive melanin production leads to skin pigmentation, which causes various cosmetic and health problems. Citrus unshiu Markovich peel (CS) and Cimicifuga dahurica (CD) have long been widely used as a oriental medicinal plant because of their pharmacological properties including anti-ulcer, anti-oxidant, and anti-inflammatory properties. Methods: In this study, we investigated the inhibitory effects of CS, CD extracts or CS:CD=1:2 mixture on melanogenesis according to the various extraction methods. CS and CD extracted were prepared by ethanol extraction (EE), ultrasonification extraction (USE), Supercritical extraction (SCE), reflux extraction (RE), respectively. Results: DPPH radical scavenging and tyrosinase inhibitory activity of CD extracts or CS:CD=1:2 mixture were increased in dose-dependent manners. In addition, we evaluated the effect of CS, CD extracts or CS:CD=1:2 mixture on tyrosinase activity and melanogenesis in α-melanocyte stimulating hormone-induced B16-F10 melanoma cells. CS, CD extracts or CS:CD=1:2 mixture significantly inhibited tyrosinase activity and melanogenesis at 10-200 μg/mL. Conclusions: Therefore, our study suggests that CS and CD extracts have potential as a safe treatment for excessive pigmentation or as a natural ingredient in cosmetics.
참고문헌 (25)
- Desmedt B, "Overview of skin whitening agents with an insight into the illegal cosmetic market in Europe", J Eur Acad Dermatol Venereol, 30(6), pp.943~950 (2016)
- Costin GE, "Human skin pigmentation : melanocytes modulate skin color in response to stress", FASEB J, 21(4), pp.976~994 (2007)
- Karunarathne WAHM, "Anthocyanins from Hibiscus syriacus L. Inhibit Melanogenesis by Activating the ERK Signaling Pathway", Biomolecules, 9(11), pp.645~ (2019)
- 이아영, "The Inhibition of Melanogenesis Via the PKA and ERK Signaling Pathways by Chlamydomonas reinhardtii Extract in B16F10 Melanoma Cells and Artificial Human Skin Equivalents", Journal of Microbiology and Biotechnology, 28(12), pp.2121~2132 (2018), 한국미생물·생명공학회
- Lee JY, "Caffeic acid phenethyl ester inhibits alpha-melanocyte stimulating hormone-induced melanin synthesis through suppressing transactivation activity of microphthalmia-associated transcription factor", J Nat Prod, 76(8), pp.1399~1405 (2013)
- 정명근, "맥문동 종실 안토시아닌 분획물의 멜라닌 생성 억제 및 미백 효과", 한국약용작물학회지, 21(5), pp.361~371 (2013), 한국약용작물학회
- 하상근, "Effect of the BuOH Soluble Fraction of Cinnamomum camphora on Melanin Biosynthesis", 한국약용작물학회지, 17(4), pp.293~300 (2009), 한국약용작물학회
- 조연옥, "피부에서의 백삼 분획물의 멜라닌 색소 저해효과", 한국약용작물학회지, 16(3), pp.192~194 (2008), 한국약용작물학회
- Roh JS, "Inhibitory effects of active compounds isolated from safflower (Carthamus tinctorius L.) seeds for melanognensis", Biological and Pharmaceutical Bulletin, 27(12), pp.1976~1978 (2004)
- Lee SH, "Mulberroside F isolated from the leaves of Morus alba inhibits melanin biosynthesis", Biological and Pharmaceutical Bulletin, 25(8), pp.1045~1048 (2002)
- Kang SI, "Immature Citrus sunki peel extract exhibits antiobesity effects by β-oxidation and lipolysis in high-fat diet-induced obese mice", Biol Pharm Bull, 35(2), pp.223~230 (2012)
- Jin YJ, "Anti-Obesity Effects of Polymethoxyflavone-Rich Fraction from Jinkyool(Citrus sunki Hort. ex Tanaka)Leaf on Obese Mice Induced by High-Fat Diet", Nutrients, 14(4), pp.865~ (2022)
- Choi YS, "Chloroform extract of Citrus unshiu Markovich peel induces apoptosis and inhibits stemness in HeLa human cervical cancer cells", Mol Med Rep, 23(1), pp.86~ (2021)
- Han NR, "Improvement effects of a mixed extract of flowers of Pueraria thomsonii Benth. and peels of Citrus unshiu Markovich on postmenopausal symptoms of ovariectomized mice", Biomed Pharmacother, 103, pp.524~530 (2018)
- Soon-Ho Yim, "Cytotoxic Caffeic Acid Derivatives from the Rhizomes of Cimicifuga heracleifolia", Archives of Pharmacal Research, 35(9), pp.1559~1565 (2012), 대한약학회
- Jia H, "Cimicifuga dahurica extract inhibits the proliferation, migration and invasion of breast cancer cells MDA-MB-231 and MCF-7 in vitro and in vivo", J Ethnopharmacol, 277, pp.114057~ (2021)
- Thao NP, "Inhibition Potential of Cycloartane-Type Glycosides from the Roots of Cimicifuga dahurica against Soluble Epoxide Hydrolase", J Nat Prod, 80(6), pp.1867~1875 (2017)
- Martinez-Esparza M, "Mechanism of melanogenesis inhibition by tumor necrosis factor-alpha in B16", F10 mouse melanoma cells, 1998, pp.255(1), 139~146
- Hosoi J, "Regulation of melanin synthesis of B16 mouse melanoma cells by 1α. 25-Dihydroxyvitamin D3and Retinoic Acid", Cancer Res, 45(4), pp.1474~1478 (1985)
- 이동숙, "편백나무 잎 추출물의 항산화 활성과 성분분석에 관한 연구", 공업화학, 23(1), pp.93~99 (2012), 한국공업화학회
- 박수아, "닥나무 추출물의 항산화 활성 및 성분 분석", 공업화학, 24(2), pp.177~183 (2013), 한국공업화학회
- Kikuchi H, "Detection of point mutation in the tyrosinase of a Japanese albino patient by a direct sequencing of amplified DNA", Hum Genet, 85(1), pp.123~124 (1990)
- Park CH, "A natural compound harmine decreases melanin synthesis through regulation of the DYRK1A", NFATC3 pathway, 2021, pp.103(1), 16~24
- Zhou S, "Citric acid promoted melanin synthesis in B16F10mouse melanoma cells, but inhibited it in human epidermal melanocytes and HMV-II melanoma cells via the GSK3β", β-catenin signaling pathway, 2020, pp.15(12), e0243565~
- Sato K, "2-Ethoxybenzamide stimulates melanin synthesis in B16F1melanoma cells via the CREB signaling pathway", Mol Cell Biochem, 423(1-2), pp.39~52 (2016)