육계에 대한 유산균의 장내 생존성 및 적정 급여방법에 대한 연구
Study on Intestinal Viability and Optimum Feeding Method of Lactobacillus in Broiler Chickens
1
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
This study was conducted to prove the optimum feeding method of Lactobacillus in broiler chickens by investigating the intestinal viability of ingested Lactobacillus and the effect of feeding levels and frequency of Lactobacillus on growth performance in broiler chickens. In experiment 1, A total of one hundred, 5 weeks old male broiler chickens(Abor Acre) were fed Lactobacillus reuteri avibro2 expressed green fluorescent protein(GFP) at 10⁴cfu/g diet to investigate the retention time of ingested Lactobacillus in the intestine for 1 day. The percentage of Lactobacillus expressed GFP in intestinal contents was 26% at 1 day after fed Lactobacillus expressed GFP. The percentage of Lactobacillus expressed GFP in intestinal contents was decreased in length of time. In experiment 2, A total of four hundred eighty, 1-d-old male broiler chicks(Abor Acre) were randomly divided into 4 groups with 4 replicates of 30 birds each to prove the optimum feeding level of Lactobacillus. The treatments were control(free antibiotics), Lactobacillus reuteri avibro2 5.0×10cfu/mL, 5.0×10³cfu/mL, and 5.0×105cfu/mL. The final body weight and body wight gain of Lactobacillus reuteri avibro2 5.0×10³cfu/mL were the highest in all groups(P<0.05). Feed conversion ratio was not significantly difference among the groups. The number of intestinal lactic acid bacteria in Lactobacillus treated groups tended to be improved or significantly increased as compared to that of control(P<0.05). Protein and fat digestibility in Lactobacillus 5.0×10³cfu/mL and 5.0×105cfu/mL treated groups were significantly improved(P<0.05). No significant differences were observed on the availability of dry matter and crude ash in Lactobacillus treatments compared to those of control. In experiment 3, A total of six hundred 1-d-old male broiler chicks(Abor Acre) were randomly divided into 4 groups with 4 replicates of 30 birds each and were fed Lactobacillus reuteri avibro2 at intervals of 1, 2, 3, and 5 day for five weeks. Feeding level of Lactobacillus was 5.0×10³cfu/mL The final body weight and body wight gain of Lactobacillus reuteri avibro2 5.0×10³cfu/mL were the highest in all groups(P<0.05). The final body weight and body weight gain were significantly increased, when Lactobacillus was fed at intervals of 1 days, or 2 days. There were no significant differences in feed intake and feed conversion ratio among the all groups. The number of intestinal lactic acid bacteria in Lactobacillus treated groups tended to be improved or significantly increased as compared to that of control(P<0.05). No significant differences were observed on the number of coliform bacteria and Salmonella of ileum and cecum. Consequently, supplemental Lactobacillus influenced positive effects on the growth performance, nutrient availability and intestinal microflora. The optimum feeding level of Lactobacillus was 5.0×10³cfu/mL, and the constant feeding of Lactobacillus was effective.
Abstract
This study was conducted to prove the optimum feeding method of Lactobacillus in broiler chickens by investigating the intestinal viability of ingested Lactobacillus and the effect of feeding levels and frequency of Lactobacillus on growth performance in broiler chickens. In experiment 1, A total of one hundred, 5 weeks old male broiler chickens(Abor Acre) were fed Lactobacillus reuteri avibro2 expressed green fluorescent protein(GFP) at 10⁴cfu/g diet to investigate the retention time of ingested Lactobacillus in the intestine for 1 day. The percentage of Lactobacillus expressed GFP in intestinal contents was 26% at 1 day after fed Lactobacillus expressed GFP. The percentage of Lactobacillus expressed GFP in intestinal contents was decreased in length of time. In experiment 2, A total of four hundred eighty, 1-d-old male broiler chicks(Abor Acre) were randomly divided into 4 groups with 4 replicates of 30 birds each to prove the optimum feeding level of Lactobacillus. The treatments were control(free antibiotics), Lactobacillus reuteri avibro2 5.0×10cfu/mL, 5.0×10³cfu/mL, and 5.0×105cfu/mL. The final body weight and body wight gain of Lactobacillus reuteri avibro2 5.0×10³cfu/mL were the highest in all groups(P<0.05). Feed conversion ratio was not significantly difference among the groups. The number of intestinal lactic acid bacteria in Lactobacillus treated groups tended to be improved or significantly increased as compared to that of control(P<0.05). Protein and fat digestibility in Lactobacillus 5.0×10³cfu/mL and 5.0×105cfu/mL treated groups were significantly improved(P<0.05). No significant differences were observed on the availability of dry matter and crude ash in Lactobacillus treatments compared to those of control. In experiment 3, A total of six hundred 1-d-old male broiler chicks(Abor Acre) were randomly divided into 4 groups with 4 replicates of 30 birds each and were fed Lactobacillus reuteri avibro2 at intervals of 1, 2, 3, and 5 day for five weeks. Feeding level of Lactobacillus was 5.0×10³cfu/mL The final body weight and body wight gain of Lactobacillus reuteri avibro2 5.0×10³cfu/mL were the highest in all groups(P<0.05). The final body weight and body weight gain were significantly increased, when Lactobacillus was fed at intervals of 1 days, or 2 days. There were no significant differences in feed intake and feed conversion ratio among the all groups. The number of intestinal lactic acid bacteria in Lactobacillus treated groups tended to be improved or significantly increased as compared to that of control(P<0.05). No significant differences were observed on the number of coliform bacteria and Salmonella of ileum and cecum. Consequently, supplemental Lactobacillus influenced positive effects on the growth performance, nutrient availability and intestinal microflora. The optimum feeding level of Lactobacillus was 5.0×10³cfu/mL, and the constant feeding of Lactobacillus was effective.
참고문헌 (37)
- AOAC, "Official method of analysis, 16th edition" (1995), Association of Official Analytical Chemists
- Adler, H. E., "Effect of ingested Lactobacilli on Salmonella infantis and Escherichia coli and on intestinal flora pasted vents and chick growth", Avian Dis, 24, pp.868~878 (1980)
- Barnes, E. M., "The occurrence and properties of uric acid decomposing anaerobic bacteria in the avian cecum", J. Appl. Bacteriol, 37, pp.393~409 (1974)
- Bongaerts, G., "Effect of antibiotics, prebiotics and probiotics in treatment for hepatic encephalopathy", Med. Hypotheses, 64, pp.64~68 (2005)
- Champ, M., "Amylase production by three Lactobacillus strains isolated from chicken crop", J. Appl. Bacteriol, 55, pp.487~ (1983)
- Duncan, D. B., "Multiple range and multiple F test", Biometric, 11, pp.1~42 (1955)
- Dunham, H. J., "Lactobacillus reuteri immunomodulation of stressor-associated disease in newly hatched chickens and turkeys", Poult. Sci, 72(Suppl 1), pp.103~ (1993)
- Fuller, R., "Ecological studies on the Lactobacillus flora associated with the crop epithelium of the fowl", J. Appl. Bacteriol, 36, pp.131~139 (1973)
- Fuller, R., "Nature of the determinant responsible for the adhesion of Lactobacilli to chicken crop epithelial cells", Fournal of General Microbiology, 87, pp.245~250 (1975)
- Fuller, R., "The importance of Lactobacilli in maintaining normal microbial balance in crop", Br. Poult. Sci, 18, pp.85~94 (1977)
- Fuller, R., "Probiotics in man and animals", J. Appl. Bacteriol, 66, pp.365~378 (1989)
- Gibson, G. R., "Aspects of in vitro and in vivo research approaches directed toward identifying probiotics and prebiotics for human use", J. Nutr, 130, pp.391~395 (2000)
- Gilliland, S. E., "Beneficial interrelationships between certain micro-organisms and humans: Candidate micro-organisms for use as dietary adjuncts", J. Food Protect, 42, pp.164~ (1979)
- Impey, C. S., "Fate of Salmonellasin the alimentary tract of chicks pre-treated with a mature caecal microflora to increase colonization resistance", J. Appl. Bacteriol, 66, pp.469~ (1989)
- Jin L. Z., "Influence of dried Bacillus subtillis and Lactobacilli cultures on intestinal microflora and performance in broilers", Asian-Australian J. Anim. Sci, 9, pp.397~403 (1996)
- Jin, L. Z., "Growth performance intestinal microbial populations and serum cholesterol of broilers fed diets containing Lactobacillus cultures", Poult. Sci, 77, pp.1259~1265 (1998)
- Lyons, T. P., "Probiotics: an alternative to antibiotics", Pig News Info., 8, pp.157~164 (1987)
- Maiolino, R., "Research on the efficiency of", ~ (1992)
- Mohan, B., "Effect of probiotic supple- mentation on growth nitrogen utilization and serum cholesterol in broilers", Br. Poult. Sci, 37, pp.395~401 (1996)
- Nahashon, S. N., "Performance of single Comb white Leghorn layers fed corn-soybean meal and barley-corn-soybean meal diets supple- mented with a direct fed microbial", Poult. Sci, 73, pp.1712~1723 (1994)
- Nutrient requirement of poultry, "NRC", National Research Council National Academy of Science Washington DC, ~ (1994)
- Nurmi, E., "New aspects of Salmonella infection in broiler production", Nature, 241, pp.210~211 (1973)
- Pirgozliev, V., "of diets containing Esherichia coli-derived phytase to young chickens and turkeys: effects on performance, metabolisable energy, endogenous secretions and intestinal morphology", Poult. Sci, 86, pp.705~713 (2007)
- Prins, R. A., "Biochemical activities of gut micro-organisms, In Microbial Ecology of the Gut", pp.73~183 (1977), Academic press
- SAS, "SAS", STAT Software for PC. Release 9.1, 2002
- Smith, M. W., "Expression of digestive and absorptive function in differentiating enterocytes", Annu. Rev. Physiol, 47, pp.247~ (1985)
- Schneitz, C., "Effect of BROILACT® on the physicochemical conditions and nutrient digestibility in the gastrointestinal tract of broilers", Poult. Sci, 77, pp.426~432 (1998)
- Stavric, S., "Role of adhering microflora in competitive exclusion of Salmonella from young chicks", J. Food Protect, 50, pp.928~ (1987)
- Tagg, J. R., "Bacteriocins of gram-positive bacteria", Bacteriological Reviews, 40, pp.722~756 (1976)
- Watkins, B. A., "In vivo effects of Lactobacillus acidophilus against pathogenic Escherichia coli in gnotobiotic chicks", Poult. Sci, 61, pp.1298~1308 (1982)
- Watkins, B. A., "Effect of oral dosing of Lactobacillus strains on gut colonization and liver biotin in broiler chicks", Poult. Sci, 62, pp.2088~2094 (1983)
- White, F., "Stomach function in relation to a scour syndrome in the piglet", Br. J. Nutr, 23, pp.847~858 (1969)
- Yeo, J., "Effect of feeding diets containing an antibiotic, a probiotic, or Yucca extract on growth and intestinal urease activity in broiler chicks", Poult. Sci, 76, pp.381~385 (1997)
- 김상호, "육계 생산성 및 맹장내 미생물에 대한 유산균의 첨가 효과", 한국가금학회지, 27, pp.37~41 (2000)
- 김세헌, "락토바실러스 균종을 위한 형질전환 방법 및 외래 단백질 발현 백터", 등록번호 10-0635450, ~ (2006)
- 박수영, "유산균의 급여가 육계의 성장능력에 미치는 영향", 한국가금학회지, 28(1), pp.27~40 (2001)
- 박수영, "유산균의 급여가 육계의 성장능력에 미치는 영향", 한국가금학회지, 28(1), pp.27~40 (2001)