General Properties of Phytase Produced by Fluorescent Pseudomonas sp. BUN1
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초록
A bacterial strain producing intracellular phytase was isolated from cultivable soil near cowsheds and identified as a fluorescent Pseudomonas sp. BUN1. The BUN1 phytase, partially purified by cation and anion exchange chromato- graphy, exhibited its optimal activity at 40℃ and pH 5.5. As for substrate specificity, it was very specific for phytate and showed little activity on other phosphorylated conjugates. Its activity was greatly inhibited by metal ions such as Cu2+, Cd2+, and Zn2+. Addition of corn starch to PSM(phytase- synthetic medium)[0.5% sodium phytate, 0.5%(NH4)2SO4, 0.5% KCl, 0.01% MgSO4․7H2O, 0.01% CaCl2․2H2O, 0.01% NaCl, 0.001% FeSO4․7H2O, 0.001% MnSO4․4H2O; pH 6.5] for the phytase production significantly induced its enzyme activity in comparison with other carbon sources tested.
Abstract
A bacterial strain producing intracellular phytase was isolated from cultivable soil near cowsheds and identified as a fluorescent Pseudomonas sp. BUN1. The BUN1 phytase, partially purified by cation and anion exchange chromato- graphy, exhibited its optimal activity at 40℃ and pH 5.5. As for substrate specificity, it was very specific for phytate and showed little activity on other phosphorylated conjugates. Its activity was greatly inhibited by metal ions such as Cu2+, Cd2+, and Zn2+. Addition of corn starch to PSM(phytase- synthetic medium)[0.5% sodium phytate, 0.5%(NH4)2SO4, 0.5% KCl, 0.01% MgSO4․7H2O, 0.01% CaCl2․2H2O, 0.01% NaCl, 0.001% FeSO4․7H2O, 0.001% MnSO4․4H2O; pH 6.5] for the phytase production significantly induced its enzyme activity in comparison with other carbon sources tested.
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