난황항체(IgY) 급여가 Holstein 송아지의 성장능력 및 면역관련 혈액 지표에 미치는 영향
Effect of Immunoglobulin Y on Growth Performance and Blood Immunological Parameters in Holstein Calves
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본 연구는 초유 급여를 마친 갓 태어난 Holstein 송아지 14두를 대조구(Con) 및 시험구(IgY)로 나누어 49일 동안 대용유를 급여하면서 난황항체(IgY구)를 1주일 동안 투여하여 사양성적, 분변 상태 및 미생물 균총, 혈액내 생화학적 성분 및 면역관련 지표인 백혈구 성상을 조사하여 가장 바람직한 송아지 포유 및 육성 방법을 조사하였다. 시험결과로서 포유 송아지에게 IgY 투여시 체중 및 증체는 대조구(Con) 및 시험구(IgY)간에 유의적 차이 없이 모두 비슷한 성적을 보였다. 송아지 분변의 대장균(E. coli) 및 유산균 총균수를 조사한 결과 IgY 투여에 따른 대장균 및 유산균 수의 차이가 나타나지 않았으나, 분의 상태는 시험구에서 7주째 유의적으로(P<0.05) 고형상태로 배설되었다. 송아지 혈액의 백혈구 감별계수(%)를 조사한 호중구, 림프구, 단핵구, 호염기구 등의 상대적 %는 모든 구에서 정상적인 백혈구 감별 계수를 보여 IgY 투여에 따른 면역반응에는 차이가 없는 것으로 관찰되었다. 또한 혈액내 존재하는 총 단백질, albumin 및 IgG 등에서도 대조구 및 시험구 모두 비슷한 수준을 나타내었다. 결론적으로 포유 송아지에서 1주 동안 IgY 투여는 증체, 면역작용 및 분변 미생물의 균총에는 특이적 영향이 없는 것으로 사료되어, 송아지에게 생후 24시간 이내 초유를 정확하게 급여하고 사양관리에 충실 할 경우 송아지의 포유 및 육성은 성공적으로 이루어 질 수 있을 것으로 사료된다.
Abstract
A total of fourteen, 1-wk-old male Holstein calves were allotted into two groups consisted of control(CON) and IGY which was orally administrated with immunoglobulin yolk(IgY) for 1wk. Calves in both groups were provided with milk replacer according to feeding program and had ad libitum access to timothy hay for the entire experimental period(7wks). At 0, 7 and 49 day of experiment, blood samples were collected from the jugular vein of calves to investigate blood biochemical profiles and the differential count(%) of white blood cell(WBC). We also monitored growth performance and colony forming unit(CFU) of fecal microbial population in calves. The adminstration of IgY in calves did not affect body weight and weight gain during 49 days feeding trial compared with control group. The CFU of E. coli and Lactobacilli in the feces of calves were not significantly affected by IgY treatment, whereas the score of the calf scours during day 43 to 49 in IgY group showed a significant(P<0.05) solid type. There were no differences in plasma biochemical components including total protein, albumin, immunoglobulin and the other indicators. As for WBC differential count(%), there was no statistical difference in the percentages of neutrophil, lymphocyte, monocyte, eosinophil and basophil at 0, 7 and 49 days after the oral supplementation of IgY. In conclusion, the oral supplementation of IgY as an immunostimulant did not affect growth performance, fecal microbial population, blood biochemical profile and WBC differential count in Holstein calves.
참고문헌 (25)
- Arthington, J. D, "Passive IgG transfer in newborn calves fed colostrum or spray-dried serum protein alone or as a supplement to colostrum of varying quality", J. Dairy Sci, 83(12), pp.2834~2838 (2000)
- Baron, J. M, "Retinoic acid and its 4-oxo metabolites are functionally active in human skin cells in vitro", J. Invest. Dermatol, 125(1), pp.143~153 (2005)
- Brown, G. B, "Defective function of leukocytes from cattle persistently infected with bovine viral diarrhea virus, and the influence of recombinant cytokines", Am. J. Vet. Res, 52, pp.381~ (1991)
- Erhard, M. H, "Systemic availability of bovine IgG and chicken IgY after feeding colostrum and whole egg powder to newborn calves", Arch. Tierernahr, 50, pp.369~380 (1997)
- Garry, F. B, "Comparison of passive IgG transfer to dairy calves fed colostrum or commercially available colostral-supplement products", J. Am. Vet. Med. Assoc, 208(1), pp.107~110
- Girard, F, "Use of virulence factor-specific egg yolk-derived IgGs as a promising alternative to antibiotics for prevention of attaching and effacing E. coli infections", FEMS Immunol. Med. Microbiol, 46(3), pp.340~350 (2006)
- Godden, S. M, "Effect of on-farm commercial batch pasteurization of colostrum on colostrum and serum IgG concentrations in dairy calves", J. Dairy Sci, 86(4), pp.1503~1512 (2003)
- Hoerlein, A, "Studieson the epizootio- logy of shipping fever in calves", J. Am. Vet. Med. Assoc, 131, pp.123~ (1957)
- Ikemori, Y, "Protection of neonatal calves against fatal enteric colibacillosis by administration of chicken egg yolk powder from hens immunized with K99-pilated enterotoxigenic", E. coli. Am. J. Vet. Res, 53, pp.2005~2008 (1992)
- Kim, D, "Etiology and clinical aspects of diarrhea of Korean native calves during the suckling periods", Kor. J. Vet. Res, 30(2), pp.255~260 (1990)
- Kim, J. M, "Development of preventive method for enterotoxigenic colibacillosis using egg yolk antibodies against colibacillosis of piglets", Korean J. Vet. Res, 34, pp.837~842 (1998)
- Logan, E. F, "Studies on the immunity of the calf to colibacillosis-VII: The experimental reproduction of enteric colibacillosis in colostrum- fed calves", Veterinary Record, 101, pp.443~446 (1977)
- Menge, C, "Compensation of preliminary blood phagocyte immaturity in the newborn calf", Vet Immunol. Immunopathol, 62(4), pp.309~321 (1998)
- Oh, T. H, "Protective effect of chicken egg yolk antibody in colostrums-deprived neonatal puppies", Korean J. Vet. Res, 36, pp.903~913 (1996)
- Osame, S, "Efficacy of colostrial immunoglobulins for therapeutic and preventive treatments of calf diarrhea", J. Vet. Med. Sci, 53(1), pp.87~91 (1991)
- Quigley, J. D, "Effects of a colostrum replacement product derived from serum on immunoglobulin G absorption by calves", J. Dairy Sci, 81(7), pp.1936~1939 (1998)
- Quigley, J. D. III, "Influence of injectable immunoglobulin on serum immunoglobulin concentra- tions in dairy calves", J. Dairy Sci, 79(11), pp.2032~2037 (1996)
- SAS, "User’s Guide: Statistics Version 6" (1996), SAS inst, Inc
- Yokoyama, H, "Passive protective effect of chicken egg yolk immnunoglobulins against experimental enterotoxigenic", E. coli infection in neonatal piglets. Infect. Immuno, 60, pp.998~1007 (1992)
- 김두, "한우송아지의 포유기간 중의 설사발생에 관한 연구", Korean J. Vet. Res, 30(2), pp.250~260 (1990)
- 백순용, "백순용의 소 질병관리" (1990), 종합출판문화사
- 정근기, "한우. 전국한우연구회" (2005), 대원문화인쇄
- 서정원, "건강한 송아지와 설사송아지에서 혈중 retinol과 α-tocopherol의 비교", 석사, pp.2006
- 양일석, "수의생리학" (2004), 광일문화사
- 內藤善久, "가축설사의 진단: 송아지 설사에 대하여", Kor. J. Vet. Clin. Med, pp.1~7 (1993)