Identification of proteins involved in the pancreatic exocrine by exogenous ghrelin administration in Sprague-Dawley rats
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The aims of study were to investigate the effects of intraperitoneal (i.p.) infusion of ghrelinon pancreatic α-amylase outputs and the responses of pancreatic proteins to ghrelin that mayrelate to the pancreatic exocrine. Six male Sprague-Dawley rats (300 g) were randomlydivided into two groups, a control group (C, n = 3) and a treatment group (T, 10.0μg/kg BW,n = 3). Blood samples were collected from rat caudal vein once time after one hour injection. The concentrations of plasma ghrelin, cholecystokinin (CCK) and alfa-amylase activity wereevaluated by enzyme immunoassay (EIA) kit. Two-dimensional gel electrophoresis (2-DE)analysis was conducted to separate the proteins in pancreas tissue. Results showed that thei.p. infusion of ghrelin at doses of 10.0 μg/kg body weight (BW) increased the plasma ghrelinconcentrations (p = 0.07) and elevated the plasma CCK level significantly (p < 0.05). Although there was no statistically significant, the α-amylase activity tended to increase. Theproteomics analysis indicated that some pancreatic proteins with various functions were upordown- regulated compared with control group. In conclusion, ghrelin may have role in thepancreatic exocrine, but the signaling pathway was still not clear. Therefore, much morefunctional studies focus on these found proteins are needed in the near future.
Abstract
The aims of study were to investigate the effects of intraperitoneal (i.p.) infusion of ghrelinon pancreatic α-amylase outputs and the responses of pancreatic proteins to ghrelin that mayrelate to the pancreatic exocrine. Six male Sprague-Dawley rats (300 g) were randomlydivided into two groups, a control group (C, n = 3) and a treatment group (T, 10.0μg/kg BW,n = 3). Blood samples were collected from rat caudal vein once time after one hour injection. The concentrations of plasma ghrelin, cholecystokinin (CCK) and alfa-amylase activity wereevaluated by enzyme immunoassay (EIA) kit. Two-dimensional gel electrophoresis (2-DE)analysis was conducted to separate the proteins in pancreas tissue. Results showed that thei.p. infusion of ghrelin at doses of 10.0 μg/kg body weight (BW) increased the plasma ghrelinconcentrations (p = 0.07) and elevated the plasma CCK level significantly (p < 0.05). Although there was no statistically significant, the α-amylase activity tended to increase. Theproteomics analysis indicated that some pancreatic proteins with various functions were upordown- regulated compared with control group. In conclusion, ghrelin may have role in thepancreatic exocrine, but the signaling pathway was still not clear. Therefore, much morefunctional studies focus on these found proteins are needed in the near future.
참고문헌 (21)
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