Effects of a lipid-encapsulated zinc oxide dietary supplement, on growth parameters and intestinal morphology in weanling pigs artificially infected with enterotoxigenic Escherichia coli
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The study was performed to investigate the effect of dietary supplementation of a lipid-encapsulated Zinc oxide ongrowth parameters and intestinal mucosal morphology piglets born to Duroc-sired Landrace × Yorkshire dams. Twenty-four 30-day-old piglets weaned at 25 days of age were orally challenged with 5 × 108 colony forming unitsof enterotoxigenic Escherichia coli (ETEC) K88 and fed one of the four diets for 7 days: (i) a nursery basal diet containing100-ppm ZnO (referred to as BASAL), (ii) BASAL supplemented with 120-ppm apramycin (referred to as ANTIBIO), (iii)BASAL with 2,400-ppm ZnO (referred to as HIGH), and BASAL containing 100-ppm lipid-encapsulated ZnO (referred toas LE). All piglets were killed at the end of the experiment for histological examination on the intestine. The resultsshowed that the average daily gain (ADG), the villus height: crypt depth (CD) ratio in the ileum, and the goblet celldensity of the villus and crypt in the duodenum, jejunum, and colon were greater in the LE-fed group that those of theBASAL (p < 0.05). Fecal consistency score (FCS) and the CD ratio in the ileum were less in the LE-fed group, comparedto the BASAL-fed one (p < 0.05). The effects observed in the LE-fed group were almost equal to those of the HIGH-fedgroup as well as even superior to those of the ANTIBIO-fed group. Taken together, our results imply that dietarysupplementation of 100-ppm lipid-encapsulated ZnO is as effective as that of 2,400-ppm ZnO for promoting growthdiarrhea and intestinal morphology caused by ETEC infection.
Abstract
The study was performed to investigate the effect of dietary supplementation of a lipid-encapsulated Zinc oxide ongrowth parameters and intestinal mucosal morphology piglets born to Duroc-sired Landrace × Yorkshire dams. Twenty-four 30-day-old piglets weaned at 25 days of age were orally challenged with 5 × 108 colony forming unitsof enterotoxigenic Escherichia coli (ETEC) K88 and fed one of the four diets for 7 days: (i) a nursery basal diet containing100-ppm ZnO (referred to as BASAL), (ii) BASAL supplemented with 120-ppm apramycin (referred to as ANTIBIO), (iii)BASAL with 2,400-ppm ZnO (referred to as HIGH), and BASAL containing 100-ppm lipid-encapsulated ZnO (referred toas LE). All piglets were killed at the end of the experiment for histological examination on the intestine. The resultsshowed that the average daily gain (ADG), the villus height: crypt depth (CD) ratio in the ileum, and the goblet celldensity of the villus and crypt in the duodenum, jejunum, and colon were greater in the LE-fed group that those of theBASAL (p < 0.05). Fecal consistency score (FCS) and the CD ratio in the ileum were less in the LE-fed group, comparedto the BASAL-fed one (p < 0.05). The effects observed in the LE-fed group were almost equal to those of the HIGH-fedgroup as well as even superior to those of the ANTIBIO-fed group. Taken together, our results imply that dietarysupplementation of 100-ppm lipid-encapsulated ZnO is as effective as that of 2,400-ppm ZnO for promoting growthdiarrhea and intestinal morphology caused by ETEC infection.
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