Expression patterns of innate immunity-related genes in response to polyinosinic:polycytidylic acid (poly[I:C]) stimulation in DF-1 chicken fibroblast cells
1
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Polyinosinic:polycytidylic acid (poly[I:C]) can stimulate Toll-like receptor 3 (TLR3) signaling pathways. In this study, DF-1 cells were treated with poly(I:C) at various concentrations and time points to examine the comparative expression patterns of innate immune response genes. The viability of DF-1 cells decreased from 77.41% to 38.68% when cells were treated different dose of poly(I:C) from 0.1 μg/mL to 100 μg/mL for 24 h respectively. The expressions of TLR3, TLR4, TLR7, TLR15, TLR21, IL1B, and IL10 were increased in dose- and time-dependent manners by poly(I:C) treatment. On the contrary, the expression patterns of interferon regulatory factors 7 (IRF7), Jun proto-oncogene, AP-1 transcription factor subunit (JUN), Nuclear Factor Kappa B Subunit 1 (NF-κB1), and IL8L2 were varied; IRF7 and IL8L2 were increasingly expressed whereas the expressions of JUN and NF-κB1 were decreased in a dose-dependent manner after they were early induced. In time-dependent analysis, IRF7 expression was significantly upregulated from 3 h to 24 h, whereas JUN and NF-κB1 expressions settled down from 6 h to 24 h after poly(I:C) treatment although they were induced at early time from 1 h to 3 h. Poly(I:C) treatment rapidly increased the expression of IL8L2 from 3 h to 6 h with a plateau at 6 h and then the expression of IL8L2 was dramatically decreased until 24 h after poly(I:C) treatment although the expression level was still higher than the non-treated control. These results may provide the basis for understanding host response to viral infection and its mimicry system in chickens.
Abstract
Polyinosinic:polycytidylic acid (poly[I:C]) can stimulate Toll-like receptor 3 (TLR3) signaling pathways. In this study, DF-1 cells were treated with poly(I:C) at various concentrations and time points to examine the comparative expression patterns of innate immune response genes. The viability of DF-1 cells decreased from 77.41% to 38.68% when cells were treated different dose of poly(I:C) from 0.1 μg/mL to 100 μg/mL for 24 h respectively. The expressions of TLR3, TLR4, TLR7, TLR15, TLR21, IL1B, and IL10 were increased in dose- and time-dependent manners by poly(I:C) treatment. On the contrary, the expression patterns of interferon regulatory factors 7 (IRF7), Jun proto-oncogene, AP-1 transcription factor subunit (JUN), Nuclear Factor Kappa B Subunit 1 (NF-κB1), and IL8L2 were varied; IRF7 and IL8L2 were increasingly expressed whereas the expressions of JUN and NF-κB1 were decreased in a dose-dependent manner after they were early induced. In time-dependent analysis, IRF7 expression was significantly upregulated from 3 h to 24 h, whereas JUN and NF-κB1 expressions settled down from 6 h to 24 h after poly(I:C) treatment although they were induced at early time from 1 h to 3 h. Poly(I:C) treatment rapidly increased the expression of IL8L2 from 3 h to 6 h with a plateau at 6 h and then the expression of IL8L2 was dramatically decreased until 24 h after poly(I:C) treatment although the expression level was still higher than the non-treated control. These results may provide the basis for understanding host response to viral infection and its mimicry system in chickens.
참고문헌 (54)
- Riera Romo M, "Innate immunity in vertebrates : an overview", Immunology, 148, pp.125~139 (2016)
- Thompson MR, "Pattern recognition receptors and the innate immune response to viral infection", Viruses, 3, pp.920~940 (2011)
- Roers A, "Recognition of endogenous nucleic acids by the innate immune system", Immunity, 44, pp.739~754 (2016)
- Majer O, "Nucleic acid-sensing TLRs : trafficking and regulation", Curr Opin Immunol, 44, pp.26~33 (2017)
- Oldenburg M, "TLR13 recognizes bacterial 23S rRNA devoid of erythromycin resistance-forming modification", Science, 337, pp.1111~1115 (2012)
- Bruns AM, "The innate immune sensor LGP2 activates antiviral signaling by regulating MDA5-RNA interaction and filament assembly", Mol Cell, 55, pp.771~781 (2014)
- Werling D, "TOLL-like receptors linking innate and adaptive immune response", Vet Immunol Immunopathol, 91, pp.1~12 (2003)
- Kaiser P, "Advances in avian immunology--prospects for disease control : a review", Avian Pathol, 39, pp.309~324 (2010)
- Turner MD, "Cytokines and chemokines : at the crossroads of cell signalling and inflammatory disease", Biochim Biophys Acta Mol Cell Res, 1843, pp.2563~2582 (2014)
- Kawai T, "Toll-like receptor and RIG-I-like receptor signaling", Ann N Y Acad Sci, 1143, pp.1~20 (2008)
- Kawai T, "Antiviral signaling through pattern recognition receptors", J Biochem, 141, pp.137~145 (2007)
- Randall RE, "Interferons and viruses : an interplay between induction, signalling, antiviral responses and virus countermeasures", J Gen Virol, 89, pp.1~47 (2008)
- Philbin VJ, "Identification and characterization of a functional, alternatively spliced Toll-like receptor 7(TLR7)and genomic disruption of TLR8 in chickens", Immunology, 114, pp.507~521 (2005)
- Temperley ND, "Evolution of the chicken Toll-like receptor gene family : a story of gene gain and gene loss", BMC Genomics, 9, pp.62~ (2008)
- Roach JC, "The evolution of vertebrate Toll-like receptors", Proc Natl Acad Sci USA, 102, pp.9577~9582 (2005)
- Higgs R, "Induction of a novel chicken toll-like receptor following Salmonella enterica serovar typhimurium infection", Infect Immun, 74, pp.1692~1698 (2006)
- Brownlie R, "Avian toll-like receptors", Cell Tissue Res, 343, pp.121~130 (2011)
- Nerren JR, "Expression of the avian-specific toll-like receptor 15 in chicken heterophils is mediated by gram-negative and gram-positive bacteria, but not TLR agonists", Vet Immunol Immunopathol, 136, pp.151~156 (2010)
- Oven I, "Diacylated lipopeptide from Mycoplasma synoviae mediates TLR15 induced innate immune responses", Vet Res, 44, pp.99~ (2013)
- Liniger M, "Chicken cells sense influenza a virus infection through MDA5 and CARDIF signaling involving LGP2", J Virol, 86, pp.705~717 (2012)
- Karpala AJ, "Characterization of chicken Mda5 activity : regulation of IFN-β in the absence of RIG-I functionality", J Immunol, 186, pp.5397~5405 (2011)
- Barber MR, "Association of RIG-I with innate immunity of ducks to influenza", Proc Natl Acad Sci USA, 107, pp.5913~5918 (2010)
- Wu J, "Poly(I : C)treatment leads to interferon-dependent clearance of hepatitis B virus in a hydrodynamic injection mouse model", J Virol, 88, pp.10421~10431 (2014)
- Fortier ME, "The viral mimic, polyinosinic:polycytidylic acid, induces fever in rats via an interleukin-1-dependent mechanism", Am J Physiol Regul Integr Comp Physiol, 287, pp.R759~R766 (2004)
- Kim TH, "Correction: functional analysis of chicken IRF7 in response to dsRNA analog poly(I:C) by integrating verexpression and knockdown", PLOS ONE, 10, pp.e0137672~ (2015)
- Liu J, "Expression and functional analysis of receptor-interacting serine", threonine kinase 2(RIP2)in Japanese flounder(Paralichthys olivaceus), 2018, pp.75, 327~335
- Bianchi F, "Exploiting poly(I : C)to induce cancer cell apoptosis", Cancer Biol Ther, 18, pp.747~756 (2017)
- Pyo S, "Poly I : C-induced antiviral and cytotoxic activities are mediated by different mechanisms", Int J Immunopharmacol, 15, pp.477~486 (1993)
- Lv Y, "Apoptosis induced in chicken embryo fibroblasts in vitro by a polyinosinic : polycytidylic acid copolymer", Toxicol In Vitro, 23, pp.1360~1364 (2009)
- Kim TH, "Functional analysis of chicken IRF7 in response to dsRNA analog poly(I:C) by integrating overexpression and knockdown", PLOS ONE, 10, pp.e0133450~ (2015)
- Livak KJ, "Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔ CT method", Methods, 25, pp.402~408 (2001)
- Molteni M, "The role of toll-like receptor 4 in infectious and noninfectious inflammation", Mediators Inflamm, 2016, pp.6978936~ (2016)
- Alexopoulou L, "Recognition of double-stranded RNA and activation of NF-kappaB by toll-like receptor 3", Nature, 413, pp.732~738 (2001)
- Hemmi H, "Small anti-viral compounds activate immune cells via the TLR7 MyD88-dependent signaling pathway", Nat Immunol, 3, pp.196~200 (2002)
- Kopp EB, "The toll-receptor family and control of innate immunity", Curr Opin Immunol, 11, pp.13~18 (1999)
- Kurt-Jones EA, "Pattern recognition receptors TLR4 and CD14 mediate response to respiratory syncytial virus", Nat Immunol, 1, pp.398~401 (2000)
- Takeuchi O, "Toll-like receptors; their physiological role and signal transduction system", Int Immunopharmacol, 1, pp.625~635 (2001)
- Takeuchi O, "Differential roles of TLR2 and TLR4 in recognition of gram-negative and gram-positive bacterial cell wall components", Immunity, 11, pp.443~451 (1999)
- Doyle S, "IRF3 mediates a TLR3", TLR4-specific antiviral gene program, 2002, pp.17, 251~263
- Yang QL, "Marek’s disease virus can infect chicken brain microglia and promote the transcription of toll-like receptor 15 and 1LB genes", Bing Du Xue Bao, 27, pp.18~25 (2011)
- Chen S, "Innate sensing of viruses by pattern recognition receptors in birds", Vet Res, 44, pp.82~ (2013)
- He H, "CpG oligodeoxynucleotide and double-stranded RNA synergize to enhance nitric oxide production and mRNA expression of inducible nitric oxide synthase, pro-inflammatory cytokines and chemokines in chicken monocytes", Innate Immun, 17, pp.137~144 (2011)
- He H, "Synergy of CpG oligodeoxynucleotide and double-stranded RNA(poly I : C)on nitric oxide induction in chicken peripheral blood monocytes", Mol Immunol, 44, pp.3234~3242 (2007)
- Karin M, "NF-kappaB : linking inflammation and immunity to cancer development and progression", Nat Rev Immunol, 5, pp.749~759 (2005)
- Theofilopoulos AN, "Type I interferons(α", β)in immunity and autoimmunity, 2005, pp.23, 307~336
- Shaulian E, "AP-1 as a regulator of cell life and death", Nat Cell Biol, 4, pp.E131~E136 (2002)
- Kawai T, "TLR signaling", Cell Death Differ, 13, pp.816~825 (2006)
- Blank V, "Cytoplasmic retention, DNA binding and processing of the NF-kappa B p50 precursor are controlled by a small region in its C-terminus", EMBO J, 10, pp.4159~4167 (1991)
- González-Navajas JM, "Immunomodulatory functions of type I interferons", Nat Rev Immunol, 12, pp.125~135 (2012)
- Hacker H, "Specificity in toll-like receptor signalling through distinct effector functions of TRAF3 and TRAF6", Nature, 439, pp.204~207 (2006)
- Pirhonen J, "Virus infection activates IL-1β and IL-18 production in human macrophages by a caspase-1-dependent pathway", J Immunol, 162, pp.7322~7329 (1999)
- Rojas JM, "IL-10 : a multifunctional cytokine in viral infections", J Immunol Res, 2017, pp.6104054~ (2017)
- Chen IY, "Response of host inflammasomes to viral infection", Trends Microbiol, 23, pp.55~63 (2015)
- 전윤홍, "Rhinovirus-Infected Epithelial Cells Produce More IL-8 and RANTES Compared With Other Respiratory Viruses", Allergy, Asthma & Immunology Research, 5(4), pp.216~223 (2013), 대한천식알레르기학회
