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DNA damage repair is suppressed in porcine aged oocytes

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초록

This study sought to evaluate DNA damage and repair in porcine postovulatory aged oocytes. The DNA damage response, which was assessed by H2A.X expression, increased in porcine aged oocytes over time. However, the aged oocytes exhibited a significant decrease in the expression of RAD51, which reflects the DNA damage repair capacity. Further experiments suggested that the DNA repair ability was suppressed by the downregulation of genes involved in the homologous recombination (HR) and nonhomologous end-joining (NHEJ) pathways. The expression levels of the cell cycle checkpoint genes, CHEK1 and CHEK2, were upregulated in porcine aged oocytes in response to induced DNA damage. Immunofluorescence results revealed that the expression level of H3K79me2 was significantly lower in porcine aged oocytes than in control oocytes. In addition, embryo quality was significantly reduced in aged oocytes, as assessed by measuring the cell proliferation capacity. Our results provide evidence that DNA damage is increased and the DNA repair ability is suppressed in porcine aged oocytes. These findings increase our understanding of the events that occur during postovulatory oocyte aging.

Abstract

This study sought to evaluate DNA damage and repair in porcine postovulatory aged oocytes. The DNA damage response, which was assessed by H2A.X expression, increased in porcine aged oocytes over time. However, the aged oocytes exhibited a significant decrease in the expression of RAD51, which reflects the DNA damage repair capacity. Further experiments suggested that the DNA repair ability was suppressed by the downregulation of genes involved in the homologous recombination (HR) and nonhomologous end-joining (NHEJ) pathways. The expression levels of the cell cycle checkpoint genes, CHEK1 and CHEK2, were upregulated in porcine aged oocytes in response to induced DNA damage. Immunofluorescence results revealed that the expression level of H3K79me2 was significantly lower in porcine aged oocytes than in control oocytes. In addition, embryo quality was significantly reduced in aged oocytes, as assessed by measuring the cell proliferation capacity. Our results provide evidence that DNA damage is increased and the DNA repair ability is suppressed in porcine aged oocytes. These findings increase our understanding of the events that occur during postovulatory oocyte aging.

참고문헌 (50)

  1. Shao-Chen Sun, "Degradation of actin nucleators affects cortical polarity of aged mouse oocytes", Fertility and Sterility, 97(4), pp.984~990 (2012), Elsevier BV[DOI]
  2. Qiu-Xia Liang, "Resveratrol increases resistance of mouse oocytes to postovulatory aging in vivo", Aging, 10(7), pp.1586~1596 (2018), Impact Journals, LLC[DOI]
  3. Yue Wang, "N-acetyl-L-cysteine (NAC) delays post-ovulatory oocyte aging in mouse", Aging, 11(7), pp.2020~2030 (2019), Impact Journals, LLC[DOI]
  4. Y.-L. Miao, "Oocyte aging: cellular and molecular changes, developmental potential and reversal possibility", Human Reproduction Update, 15(5), pp.573~585 (2009), Oxford University Press (OUP)[DOI]
  5. Michael J. Bertoldo, "NAD+ Repletion Rescues Female Fertility during Reproductive Aging", Cell Reports, 30(6), pp.1670~1681 (2020), Elsevier BV[DOI]
  6. A. J. Wilcox, "Post-ovulatory ageing of the human oocyte and embryo failure", Human Reproduction, 13(2), pp.394~397 (1998), Oxford University Press (OUP)[DOI]
  7. Mei-Ju Liu, "Resveratrol improves in vitro maturation of oocytes in aged mice and humans", Fertility and Sterility, 109(5), pp.900~907 (2018), Elsevier BV[DOI]
  8. Tao Wang, "Melatonin prevents postovulatory oocyte aging and promotes subsequent embryonic development in the pig", Aging, 9(6), pp.1552~1564 (2017), Impact Journals, LLC[DOI]
  9. Shuang Liang, "Effect and possible mechanisms of melatonin treatment on the quality and developmental potential of aged bovine oocytes", Reproduction, Fertility and Development, 29(9), pp.1821~1831 (2017), CSIRO Publishing[DOI]
  10. Zhe Xu, "Spontaneous activation of ovulated mouse eggs: time-dependent effects on M-phase exit, cortical granule exocytosis, maternal messenger ribonucleic acid recruitment, and inositol 1,4,5-trisphosphate sensitivity", Biology of Reproduction, 57(4), pp.743~750 (1997), Oxford University Press (OUP)[DOI]
  11. Yiliang Miao, "Fate of the first polar bodies in mouse oocytes", Molecular Reproduction and Development, 69(1), pp.66~76 (2004), Wiley[DOI]
  12. Tessa Lord, "Melatonin prevents postovulatory oocyte aging in the mouse and extends the window for optimal fertilization in vitro", Biology of Reproduction, 88(3), pp.67~ (2013), Oxford University Press (OUP)[DOI]
  13. Tom Ducibella, "Changes in the Distribution of Mouse Oocyte Cortical Granules and Ability to Undergo the Cortical Reaction during Gonadotropin-stimulated Meiotic Maturation and Aging in Vivo", Biology of Reproduction, 43(5), pp.870~876 (1990), Oxford University Press (OUP)[DOI]
  14. Kim NH, "Cytoskeletal alteration in aged porcine oocytes and parthenogenesis", Mol Reprod Dev, 43, pp.513~518 (1996)[DOI]
  15. Anuradha P Goud, "Dynamic changes in microtubular cytoskeleton of human postmature oocytes revert after ooplasm transfer", Fertility and Sterility, 81(2), pp.323~331 (2004), Elsevier BV[DOI]
  16. Miroslav Hornak, "Frequency of Aneuploidy Related to Age in Porcine Oocytes", PLoS ONE, 6(4), pp.e18892~ (2011), Public Library of Science (PLoS)[DOI]
  17. RA Fissore, "Mechanisms underlying oocyte activation and postovulatory ageing", Reproduction, 124, pp.745~754 (2002), Bioscientifica[DOI]
  18. Ying-Jie Niu, "Ubiquinol-10 delays postovulatory oocyte aging by improving mitochondrial renewal in pigs", Aging, 12(2), pp.1256~1271 (2020), Impact Journals, LLC[DOI]
  19. X. Cindy Tian, "Association of MPF, MAPK, and nuclear progression dynamics during activation of young and aged bovine oocytes", Molecular Reproduction and Development, 62(1), pp.132~138 (2002), Wiley[DOI]
  20. Tessa Lord, "Oxidative stress and ageing of the post-ovulatory oocyte", REPRODUCTION, 146(6), pp.R217~R227 (2013), Bioscientifica[DOI]
  21. Gloria I. Perez, "A central role for ceramide in the age‐related acceleration of apoptosis in the female germline", The FASEB Journal, 19(7), pp.860~862 (2005), Wiley[DOI]
  22. Xing-Wei Liang, "Effect of postovulatory oocyte aging on DNA methylation imprinting acquisition in offspring oocytes", Fertility and Sterility, 96(6), pp.1479~1484 (2011), Elsevier BV[DOI]
  23. Zhao-Jia Ge, "Oocyte ageing and epigenetics", REPRODUCTION, 149(3), pp.R103~R114 (2015), Bioscientifica[DOI]
  24. Kutluk Oktay, "BRCA Mutations, DNA Repair Deficiency, and Ovarian Aging1", Biology of Reproduction, 93(3), pp.67~ (2015), Oxford University Press (OUP)[DOI]
  25. Rodrigo Camponogara Bohrer, "The Incidence of DNA Double-Strand Breaks Is Higher in Late-Cleaving and Less Developmentally Competent Porcine Embryos1", Biology of Reproduction, 93(3), pp.59~ (2015), Oxford University Press (OUP)[DOI]
  26. Jacob Falck, "The ATM–Chk2–Cdc25A checkpoint pathway guards against radioresistant DNA synthesis", Nature, 410(6830), pp.842~847 (2001), Springer Science and Business Media LLC[DOI]
  27. Stephanie Panier, "Double-strand break repair: 53BP1 comes into focus", Nature Reviews Molecular Cell Biology, 15(1), pp.7~18 (2014), Springer Science and Business Media LLC[DOI]
  28. Sandeep Burma, "ATM Phosphorylates Histone H2AX in Response to DNA Double-strand Breaks", Journal of Biological Chemistry, 276(45), pp.42462~42467 (2001), Elsevier BV[DOI]
  29. Thomas Stiff, "ATR-dependent phosphorylation and activation of ATM in response to UV treatment or replication fork stalling", The EMBO Journal, 25(24), pp.5775~5782 (2006), Wiley[DOI]
  30. Tanya T Paull, "A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage", Current Biology, 10(15), pp.886~895 (2000), Elsevier BV[DOI]
  31. Rodrigo Camponogara Bohrer, "Inhibition of histone deacetylases enhances DNA damage repair in SCNT embryos", Cell Cycle, 13(13), pp.2138~2148 (2014), Informa UK Limited[DOI]
  32. Dongdong Zhang, "Increased DNA damage and repair deficiency in granulosa cells are associated with ovarian aging in rhesus monkey", Journal of Assisted Reproduction and Genetics, 32(7), pp.1069~1078 (2015), Springer Science and Business Media LLC[DOI]
  33. Shiny Titus, "Impairment of BRCA1-Related DNA Double-Strand Break Repair Leads to Ovarian Aging in Mice and Humans", Science Translational Medicine, 5(172), pp.172ra21~ (2013), American Association for the Advancement of Science (AAAS)[DOI]
  34. Mianqun Zhang, "Coenzyme Q10 ameliorates the quality of postovulatory aged oocytes by suppressing DNA damage and apoptosis", Free Radical Biology and Medicine, 143, pp.84~94 (2019), Elsevier BV[DOI]
  35. Yun‐Gwi Park, "Allicin protects porcine oocytes against damage during aging in vitro", Molecular Reproduction and Development, 86(9), pp.1116~1125 (2019), Wiley[DOI]
  36. Tao Lin, "Melatonin supplementation during prolonged in vitro maturation improves the quality and development of poor-quality porcine oocytes via anti-oxidative and anti-apoptotic effects", Molecular Reproduction and Development, 85(8-9), pp.665~681 (2018), Wiley[DOI]
  37. Kenneth J. Livak, "Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method", Methods, 25(4), pp.402~408 (2001), Elsevier BV[DOI]
  38. William M. Bonner, "γH2AX and cancer", Nature Reviews Cancer, 8(12), pp.957~967 (2008), Springer Science and Business Media LLC[DOI]
  39. A. Kinner, "γ-H2AX in recognition and signaling of DNA double-strand breaks in the context of chromatin", Nucleic Acids Research, 36(17), pp.5678~5694 (2008), Oxford University Press (OUP)[DOI]
  40. T. Zhang, "Effects of DNA damage and short-term spindle disruption on oocyte meiotic maturation", Histochemistry and Cell Biology, 142(2), pp.185~194 (2014), Springer Science and Business Media LLC[DOI]
  41. Tohru Yamamori, "ER stress suppresses DNA double-strand break repair and sensitizes tumor cells to ionizing radiation by stimulating proteasomal degradation of Rad51", FEBS Letters, 587(20), pp.3348~3353 (2013), Wiley[DOI]
  42. Vijayakumar Govindaraj, "Changes in the expression of DNA double strand break repair genes in primordial follicles from immature and aged rats", Reproductive BioMedicine Online, 30(3), pp.303~310 (2015), Elsevier BV[DOI]
  43. Jiri Bartek, "Chk1 and Chk2 kinases in checkpoint control and cancer", Cancer Cell, 3(5), pp.421~429 (2003), Elsevier BV[DOI]
  44. H. Christian Reinhardt, "Phospho-Ser", Thr-binding domains: navigating the cell cycle and DNA damage response, 2013, pp.14(9), 563~580[DOI]
  45. Zhong-Wei Wang, "Laser microbeam-induced DNA damage inhibits cell division in fertilized eggs and early embryos", Cell Cycle, 12(20), pp.3336~3344 (2013), Informa UK Limited[DOI]
  46. Shiny Titus, "Impaired DNA Repair as a Mechanism for Oocyte Aging: Is It Epigenetically Determined?", Seminars in Reproductive Medicine, 33(06), pp.384~388 (2015), Georg Thieme Verlag KG[DOI]
  47. Cyrus Martin, "The diverse functions of histone lysine methylation", Nature Reviews Molecular Cell Biology, 6(11), pp.838~849 (2005), Springer Science and Business Media LLC[DOI]
  48. Masatoshi Ooga, "Changes in H3K79 Methylation During Preimplantation Development in Mice", Biology of Reproduction, 78(3), pp.413~424 (2008), Oxford University Press (OUP)[DOI]
  49. Yentram Huyen, "Methylated lysine 79 of histone H3 targets 53BP1 to DNA double-strand breaks", Nature, 432(7015), pp.406~411 (2004), Springer Science and Business Media LLC[DOI]
  50. Tao Lin, "Delayed blastocyst formation or an extra day culture increases apoptosis in pig blastocysts", Animal Reproduction Science, 185, pp.128~139 (2017), Elsevier BV[DOI]

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