High through screening(HTS)를 이용한 白鼠의 脾臟細胞에서의 IL-5 분비 조절 韓藥物 Screening

Abstract

Backgrounds and Objectives: Asthma is considered to be an inflammatory disease characterized by airway hyperresponsiveness and pulmonary eosinophilia. And it is known the structure and function of IL-5, its receptor and the mechnism IL-5 triggered eosinophil accumulation and inflammaion of the airways. At this point of view, we assume which oriental materia medica can the splenocyte inhibit from secreting the IL-5 in vitro. Material and Methods: We used the splenocyte of mouse 8 weeks after its birth, and then cultivated those into the 2 experimental groups and control group for 48 hours. The culture medium of experimental groups were made of 1㎍/㎖, 10㎍/㎖oriental materia medica, representative. And the culture media of control group was given no oriental materia medica. Then, we assayed the quantity of cytokine-expression by the Sandwich ELISA. The quantities of cytokine-expression of the experimental groups were compared with that of the control group which was standardized. These method were used for the all of oriental materia medica treated. Results: In this study, we demonstrated that 12 oriental materia medica that inhibit the splenocyte from secreting the IL-5 in both 1㎍/㎖ and 10㎍/㎖ culture media. Those were Equiseti Herba, Sophorae Subprostratae Radix, Moutan Radicis Cortex, Trichosanthis Radix, Buddleiae Flos, Cyperi Rhizoma, Benincasae Semen, Armeniacae Semen, Zedoariae Rhizoma, Astragali Semen, Dolichoris Semen, Lilii Bulbus, Asparagi Radix, Atractylodes Rhizoma White, Polygonati Officinallis Rhizoma. Conculusions: These findings indicate that some oriental materia medica, specially Antipyretics, Herbs for Resolving Phlegm, Relieving Cough and Calming Wheezing and Herbs for Tonifying and Invigorating effects inhibit the splenocyte from secreting the IL-5. And further study experimented in vivo is needed for treating IL-5-driven inflammatory disease including asthma.