수산물 중 penicillin계열 항생제의 분석
Analysis of Penicillin Antibiotics in Aquatic Products
0
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
A simple, rapid method for determining amoxicillin (AMO) and ampicillin (AMP) in aquatic products (flatfish, salmon, shrimp, tilapia, and yellow croaker) was evaluated. For quantification, the AMO and AMP ions at m/z 348.9 and 105.9, respectively, were selected. The limit of detection (LOD) and limit of quantification (LOQ) for detecting AMO were 0.09 and 0.25 μg/L, respectively. The respective values for AMP were 0.02 and 0.05 μg/L. After 100 μg/L AMO treatment, the level decreased 10% after 7 days at 5℃, while it decreased 20% at 25℃. After 7 days, 94.9–100% of 100 μg/L AMP remained after storage at 5℃, while 62.3–100% remained after storage at 25℃. Using the food code method, the recovery of AMO ranged from 64.1–92.0% and that of AMP from 44.8–86.2%. With a protein centrifugation method, the recovery of AMO ranged from 39.8–87.9% and that of AMP from 78.0–98.1%. With liquid-liquid extraction, the recovery of AMO ranged from 36.5–88.3% and that of AMP from 31.8–75.1%.
Abstract
A simple, rapid method for determining amoxicillin (AMO) and ampicillin (AMP) in aquatic products (flatfish, salmon, shrimp, tilapia, and yellow croaker) was evaluated. For quantification, the AMO and AMP ions at m/z 348.9 and 105.9, respectively, were selected. The limit of detection (LOD) and limit of quantification (LOQ) for detecting AMO were 0.09 and 0.25 μg/L, respectively. The respective values for AMP were 0.02 and 0.05 μg/L. After 100 μg/L AMO treatment, the level decreased 10% after 7 days at 5℃, while it decreased 20% at 25℃. After 7 days, 94.9–100% of 100 μg/L AMP remained after storage at 5℃, while 62.3–100% remained after storage at 25℃. Using the food code method, the recovery of AMO ranged from 64.1–92.0% and that of AMP from 44.8–86.2%. With a protein centrifugation method, the recovery of AMO ranged from 39.8–87.9% and that of AMP from 78.0–98.1%. With liquid-liquid extraction, the recovery of AMO ranged from 36.5–88.3% and that of AMP from 31.8–75.1%.
참고문헌 (25)
- Ang CYW, "Determination of amoxicillin in catfish and salmon tissues by liquid chromatography with precolumn formaldehyde derivatization", J AOAC Int, 79, pp.389~396 (1996)
- "Annex I, 1.2. Antibiotics, maximum residue limits"[URL]
- 최정은, "국내에서 유통 중인 축산환경개선제의 표기사항 및 항생제 내성 현황조사", 한국미생물·생명공학회지, 37(3), pp.258~265 (2009), 한국미생물·생명공학회
- De Baere S, "Quantitative analysis of amoxicillin and its major metabolites in animal tissues by liquid chromatography combined with electrospray ionization tandem mass spectrometry", Anal Chem, 74, pp.1393~1401 (2002)
- "Unavoidable contaminants 2002 FSIS National residue program, Residue limits for veterinary drug"[URL]
- Jo MR, "Simultaneous determination of amoxicillin and ampicillin in fish meat using high-performance liquid chromatography", J Kor Fish Soc, 39, pp.454~459 (2006)
- Harris DC, "Quantitative chemical analysis 7th ed", pp.107~ (2007), Free-dom academy publishing Co.
- Verterinary drug residue monitoring, "KFDA (Korea Food and Drug Administration)", KFDA, 530~531 (2006)
- Monitoring of antimicrobial resistance on the food animals and meats, "KFDA (Korean Food and Drug Administration)", KFDA, 1~110 (2007)
- KFIA (Korea Foods Industry Association), "Food Code", pp.1079~1081 (2010), KFIA Press
- 김희연, "식품 중 아목시실린, 암피실린, 옥소린산 및 플루메퀸의 분석", 한국식품과학회지, 41(5), pp.490~497 (2009), 한국식품과학회
- Lee UJ, "Pharmacology lecture of Lee uh joo", pp.572~575 (2001), Medrang press
- Lim BS, "Β-lactam antibiotic fermentation and market trading", Tech News BRIEF, pp.1~2 (2004)
- Luo W, "Determination of amoxicillin residues in animal tissues by solid-phase extraction and liquid chromatography with fluorescence detection", J AOAC Int, 83, pp.20~25 (2000)
- Nagata T, "Determination of ampicillin residues in fish tissues by liquid chromatography", J Assoc Off Anal Chem, 69, pp.448~450 (1986)
- 2009 veterinary drug residues monitoring, "NIFDS (National Institute of Food and Drug Safety Evaluation)", NIFDS, 4~6 (2009)
- 오재호, "식품 중 잔류동물용의약품의 안전관리", 한국환경농학회지, 28(3), pp.310~325 (2009), 한국환경농학회
- Reyns T, "Rapid method for the quantification of amoxicillin and its major metabolites in pig tissues by liquid chromatography-tandem mass spectrometry with emphasis on stability issues", J Chromatogr B, 861, pp.108~116 (2008)
- Shitandi A, "Factors contributing to the occurrence of antimicrobial drug residues in Kenyan milk", J Food Prot, 67, pp.399~402 (2004)
- Sϕrensen LK, "Determination of amoxicillin in trout by liquid chromatography with UV detection after derivatization", J AOAC Int, 82, pp.1345~1352 (1999)
- Straub RF, "Determination of penicillin-G, ampicillin, amoxicillin, cloxacillin and cephapirin by electrospray mass-spectrometry", J Chromatogr, 647, pp.167~181 (1993)
- Ternandez-Torres R, "Application of enzymatic probe sonication extraction for the determination of selected veterinary antibiotics and their main metabolites in fish and mussel samples", Analytica Chimica Acta, 675, pp.156~164 (2010)
- Yoon KH, "Simultaneous determination of amoxicillin and clavulanic acid in human plasma by HPLC-ESI mass spectrometry", J Chromatogr B, 813, pp.121~127 (2004)
- Wang J, "Confirmatory determination of six penicillins in honey by liquid chromatography", electrospray ionization-tandem mass spectrometry, 2004, pp.87, 45~55
- Wise R, "Antimicrobial resistance: priorities for action", J Antimicrob Chemoth, 49, pp.585~586 (2002)