RSM을 이용한 홍게 (Chionoecetes japonicus) 자숙부산물의 최적 효소가수분해 조건
Optimal Conditions for Enzymatic Hydrolysate of Snow Crab Chionoecetes japonicus Cooker Effluent Using Response Surface Methodology
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참고문헌
키워드
초록
This study was performed to determine the optimal hydrolysis conditions for the production of a flavoring from the precipitation of snow crab cooker effluent (PSCCE) with commercial proteases. Based on cost-per-enzyme activity and sensory evaluations, Flavourzyme^® 500 MG plus Protamex^® (1:1 ratio, w/w) were selected as suitable enzymes. Three independent variables consisting of the substrate concentration (S), enzyme-to-substrate ratio (E/S), and hydrolysis time (T) were examined using response surface methodology (RSM). A model equation obtained from RSM was used to predict the degree of hydrolysis (DH) as follows: % DH = 52.285 - 6.371[S] + 5.469[E/S] + 7.599[T] - 5.818[S]^2 - 5.633[E/S]^2 - 6.528[T]^2 - 3.265[E/S][S] - 5.415[T][S] + 4.315[T][E/S]. From the ridge analysis, the conditions favoring the highest degree of hydrolysis were pH 7.45, 55℃, a S of 21.82%, an E/S of 0.50%, and a T of 3.74 h.
Abstract
This study was performed to determine the optimal hydrolysis conditions for the production of a flavoring from the precipitation of snow crab cooker effluent (PSCCE) with commercial proteases. Based on cost-per-enzyme activity and sensory evaluations, Flavourzyme^® 500 MG plus Protamex^® (1:1 ratio, w/w) were selected as suitable enzymes. Three independent variables consisting of the substrate concentration (S), enzyme-to-substrate ratio (E/S), and hydrolysis time (T) were examined using response surface methodology (RSM). A model equation obtained from RSM was used to predict the degree of hydrolysis (DH) as follows: % DH = 52.285 - 6.371[S] + 5.469[E/S] + 7.599[T] - 5.818[S]^2 - 5.633[E/S]^2 - 6.528[T]^2 - 3.265[E/S][S] - 5.415[T][S] + 4.315[T][E/S]. From the ridge analysis, the conditions favoring the highest degree of hydrolysis were pH 7.45, 55℃, a S of 21.82%, an E/S of 0.50%, and a T of 3.74 h.
참고문헌 (12)
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