홍바리(Epinephelus fasciatus) FSHβ와 LHβsubunit의 분리 및 동정
Isolation and Identification of FSHβ and LHβ Subunits in the Blacktip Grouper Epinephelus fasciatus
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We cloned and sequenced the cDNA encoding the FSHβ and LHβ subunits from the pituitary of the blacktip grouper Epinephelus fasciatus, which regulate vitellogenesis and maturation in vertebrates, to achieve stable and healthy gametes. The full-length cDNAs of FSHβ and LHβ were 571 bp and 617 bp, encoding 120 amino acid (aa) and 147 aa proteins, respectively. The deduced amino acid sequences of FSHβ and LHβ were highly homologous (68-97%) to those of other Perciformes; E. bruneus, Dicentrarchus labrax, Thunnus thynnus, and Pseudolabrus sieboldi. Phylogenetic analysis showed that the deduced FSHβ and LHβ amino acid sequences were categorized as a distinct subunit in the GTHβ family, and are closely related to the teleostei FSHβ and LHβ, respectively. FSHβ and LHβ mRNA exhibited high abundance in the pituitary gland and low in other brain areas, but were not present in peripheral tissues, as determined by RT-PCR.
Abstract
We cloned and sequenced the cDNA encoding the FSHβ and LHβ subunits from the pituitary of the blacktip grouper Epinephelus fasciatus, which regulate vitellogenesis and maturation in vertebrates, to achieve stable and healthy gametes. The full-length cDNAs of FSHβ and LHβ were 571 bp and 617 bp, encoding 120 amino acid (aa) and 147 aa proteins, respectively. The deduced amino acid sequences of FSHβ and LHβ were highly homologous (68-97%) to those of other Perciformes; E. bruneus, Dicentrarchus labrax, Thunnus thynnus, and Pseudolabrus sieboldi. Phylogenetic analysis showed that the deduced FSHβ and LHβ amino acid sequences were categorized as a distinct subunit in the GTHβ family, and are closely related to the teleostei FSHβ and LHβ, respectively. FSHβ and LHβ mRNA exhibited high abundance in the pituitary gland and low in other brain areas, but were not present in peripheral tissues, as determined by RT-PCR.
참고문헌 (31)
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