한우와 젖소 초유로부터 분리한 Lactoferrin과 가수분해물의 항균활성
Antimicrobial Activities of Lactoferrin and its Hydrolysate Obtained from the Colostrum of Hanwoo and Holstein Cattle
0
조회
0
다운로드
2
인용
0
참고문헌
초록
본 연구는 우리나라 재래종인 한우로부터 초유를 얻어 Lf을 분리정제한 후 한우 Lf와 젖소 Lf의 항균활성을 확인하였다. E. coli O111및 기타 미생물에 대한 항균성은 젖소 Lf가 한우 Lf 보다 높았으며, 젖소 Lf 가수분해물도 한우 Lf 가수분해물보다 마찬가지로 높았다. MIC에서는 E. coli O111 경우 젖소 Lf가 1.5mg/ml, 한우 Lf은 2.75mg/ml이며 젖소 Lf 가수분해물은 0.12mg/ml, 한우 Lf 가수분해물은 0.25mg/ml로 항균성 실험과 동일하게 젖소 Lf 가수분해물의 항균활성이 더 높은 것으로 나타났다. 한편 Lf과 lysozyme의 첨가는 항균활성을 상승시키는 효과를 나타내었다.
Abstract
The purpose of this study is to demonstrate antimicrobial activities of the lactoferrin and its peptic hydrolysates obtained from the colostrums of Hanwoo(Korean native cattle) and Holstein cattle. In the measurement of antimicrobial activity to E. coli O111 and other microorganisms, bovine lactoferrin showed a higher antimicrobial activity than that of Hanwoo cow's lactoferrin . The minimal inhibitory concentration (MIC) of lactoferrin against E. coli O111 was exhibited 1.5mg/ml(Holstein) and 2.75mg/ml (Hanwoo). The same result was also observed between bovine lactoferrin hydrolysate (0.12mg/ml) and Hanwoo cow's lactoferrin hydrolysate (0.25mg/ml). In addition of lysozyme, antimicrobial activity of lactoferrin was increased.
참고문헌 (21)
- "A bactericidal effect for human lactoferrin", pp.263~265 (1977)
- "Antibacterial spectrum of lactoferricin B a potent bactericidal peptide derived from the N-terminal region of bovine lactoferrin", pp.472~479 (1992)
- "Killing of Candida albicans by lactoferricin B a potent antimicrobial peptide derived from the N-terminal region of bovine lactoferrin", immu(l), pp.97~105 (1993)
- "Antifungal properties of lactoferricin B a peptide derived from the N-terminal region of bovine lactoferrin", pp.230~233 (1994)
- "Metal proteins with non-redox roles" (1985), Macmillan Press
- "Specificity and modulation of the action of lactoferrin a negative feedback regulator of myelopoiesis", pp.324~33 (1980)
- "The role of lactoferrin in the bactericidal function of polymorphonuclear leucocytes", 36, pp.781~791 (1979)
- "Microfiltration affinity purification of lactoferrin and immunoglobulin G from cheese whey", 56, pp.701~706 (1991)
- "Extraction of lactoperoxidase and lactoferrin from whey using batch ion exchange techniques", (v), pp.72~76 (1991)
- "Killing of gram-negative bacteria by lactoferrin and lysozyme", 88, pp.1080~1091 (1991)
- "Review of the progress of dairy science human and artificial milks for infant feeding", pp.519~554 (1981)
- "Determination of phylogenetic relationships of Korean native and Cheju nativ cttle to other breeds using PCR-RFLP of mtDNA D-loop region", 40, pp.335~344 (1998)
- "Cytidine deaminase activity in synovial fluid of patients with rheumatoid arthritis relation to lactoferrin", pp.594~597 (1990)
- "The biological role of lactoferrin", and simo(vits), pp.3~12 (1985)
- "Primary structure of the human melanoma- associated antigen p97 deduced from the mRNA sequence", mela(transferrin), pp.1261~1265 (1986)
- "Collaborative bacteriostatic activity of bovine lactoferrin with lysozyme against Escherichia coli O111", and dko(gi), pp.1705~1707 (1989)
- "Potent antibacterial peptides generated by pepsin digestion of bovine lactoferrin", pp.4137~4142 (1991)
- "the active peptide of lactoferrin", pp.585~591 (1994)
- "Lactoferrampin: a novel antimicrobial peptide in the N1-domain of bovine lactoferrin.", 25, pp.177~183 (2004)
- "Antibacterial susceptibility tests: Dilution and diffusion methods. : In Manual of clinical microbiology. P. R. Murray, E. J. Baron, M. A. Pfaller, F. C. Tenover, and R. H. Yolken (Ed.), 6th ed.,", pp.1327~1341 (1995), ASM Press
- "Biochemical properties of lactoferrins from Korean native cow and bovine colostrum", 25, pp.98~102 (2005)