돼지에서 정액 성상 및 인공수정 분만율과 염색질 구조 분석(SCSA)과의 상관관계에 관한 연구
Studies on Correlation Among Sperm Characteristics, Farrowing Rates by AI and Chromatin Structure in Boars
1
조회
0
다운로드
0
인용
0
참고문헌
초록
본 연구는 돼지에서 정자 SCSA와 정자 운동성 및 인공수정을 이용한 분만율과의 상관관계를 조사하기 위해 인공수정센터에서 정액 생산용으로 이용되고 있는 종모돈 중 개체별 정액을 이용한 인공수정 번식성적을 가지고 있는 종모돈 26두의 정액 성상 기록과 번식성적을 분석 하였고, 액상정액을 공시하여 정자운동성 분석과 SCSA를 실시하였다. 종모돈의 정액 채취 횟수에 따른 SCSA 분석은 종모돈 26두를 공시하여 정액을 8주간 6회 채취하여 공시하였다. 종모돈은 인공수정 분만율(80% 이상 ; 종모돈 7두, AI 256복, 8070% ; 종모돈 9두, AI 745복 및 70% 미만 ; 종모돈 10두, AI 293 복)에 따라 3개 군으로 구분 하였다. 액상 정액의 CASA 분석 결과는 분만율에 따른 종모돈 군 간에 유의적인 차이는 인정되지 않았다. 종모돈의 SCSA 결과는 분만율이 높을수록 COMP αt, %Red, %Peak R 및 %Mean R 값은 낮게 나타났고, 분만율이 가장 낮은 종모돈 군과 다른 두 군간에 유의적인 차이를 보였다(P<0.05). 종모돈 정액을 채취 차수에 따른 정액의 성상과 SCSA를 조사한 결과에서 채취 차수에 따른 차이는 나타나지 않았다. 종모돈의 인공수정 분만율과 SCSA 결과와의 상관관계는 COMP αt, SD αt, %Red, %Peak R, Mean R는 모두 부의 상관관계를 나타냈다. 이상의 결과에서 SCSA는 종모돈 정액의 수정 능력을 평가하고 예측하는데 유용한 방법으로 사료된다.
Abstract
author : C. K. Kim, Dep. of Animal Science and Technology, College of Industial Sciences, Chung-Ang Universit Ansung-Si Kyunggi-Do 456-756 Korea.Tel : 031-670-3026 E-mail : ckkim7503@cau.ac.kr
참고문헌 (40)
- "Correlation between sperm motility and sperm chromatin structure assay parameters", 80, pp.1404~1412 (2003)
- "Correlations between two markers of sperm DNA integrity, DNA denaturation and DNA fragmentation, in fertile and infertile men", 75, pp.674~677 (2001)
- "Sperm chromatin structure assay of bulls qualified for artificial insemination", 56, pp.557~567 (2001)
- "Effect of bovine oviduct epithelial cell apical plasma membranes on sperm function assessed by a novel flow cytometric approach", 67, pp.1125~1132 (2002)
- "Chromatin remodelling during spermiogenesis a possible role for the transition proteins in DNA strand break repair", 514, pp.111~114 (2002)
- "Comparative flow cytometric analysis of the human sperm acrosome reaction using CD46 antibody and lectins", 14, pp.111~119 (1997)
- "Protamine 2 deficiency leads to sperm DNA damage and embryo death in mice", 69, pp.211~217 (2003)
- "Fluorescence- labeled fucolectins are superior markers for flow cytometric quantization of the human sperm acrosome reaction", 65, pp.843~851 (1996)
- "Sperm chromatin structure assay is useful for fertility assessment", 22, pp.169~189 (2000)
- "Sperm chromatin structure assay is useful for fertility assessment", 78, pp.S191.~ (2002)
- "Acridine orange induced precipitation of mouse testicular sperm cell DNA reveals new patterns of chromatin structure", pp.328~334 (1990)
- "Glutathione depletion potentiates ethyl methane sulfonate induced damage to sperm chromatin structure", pp.297~304 (1993)
- "Utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic", 14, pp.1039~1049 (1999)
- "Sperm chromatin structure assay : its clinical use for detecting sperm DNA fragmentation in male infertility and comparison with other techniques", 23, pp.25~43 (2002)
- "Relation of mammalian sperm chromatin heterogeneity to fertility", pp.1131~1133 (1980)
- "Simultaneous measurement by flow cytometry of sperm cell viability and mitochondrial membrane potential related to cell motility", pp.279~280 (1982)
- "Sperm chromatin.", 45, pp.215~225 (2000)
- "Sperm factors related to in vitro penetration of porcine oocytes", 54, pp.1343~1357 (2000)
- "Prediction of porcine semen fertility by homologous in vitro penetration (hIVP) assay.", 54, pp.95~108 (1998)
- "Quality of semen stored at +15", 16°C is related to fertility of artificially inseminated swine, 1988, pp.therioge(logy), 1185~1190
- "Viability assessment of mammalian sperm using SYBR-14 and propidium iodide", 53, pp.276~284 (1995)
- "Flow cytometric evaluation of sperm parameters in relation to fertility potential", 63, pp.445~457 (2005)
- "Redefining the relationship between sperm DNA fragmentation as measured by the sperm chromatin structure assay and outcomes of assisted reproductive techniques(ART).", 82, pp.46~ (2004)
- "Correlations between heterospermic fertility and assays of porcine seminal quality before and after cryopreservation", therioge(logy), pp.385~399 (1989)
- "Chromatin structural changes in sperm after scrotal insulation of Holstein bulls", 5, pp.549~555 (1997)
- "Effects of egg yolk-citrate and milk extenders on chromatin structure and viability of cyopreserved bull sperm", pp.3836~3848 (1991)
- "Sperm chromatin structure assay parameters as predictors of failed pregnancy following assisted reproductive techniques", 15, pp.1717~1722 (2000)
- "The relationship of increased susceptibility of sperm DNA to denaturation and fertility in the stallion", 50, pp.955~972 (1998)
- "Relationship of seminal plasma level and extender type to sperm motility and DNA integrity", 63, pp.1584~1591 (2005)
- "Effect of storage time and temperature on stallion sperm DNA and fertility", 57, pp.1135~1142 (2002)
- "Study of mitochondrial membrane potential DNA fragmentation and cell viability by flow cytometry in human sperm", 17, pp.1257~1265 (2002)
- "A triple stain flow cytometric method to assess plasma and acrosome membrane integrity of cryopreserved bovine sperm immediately after thawing in presence of egg yolk particles", 68, pp.1828~1835 (2003)
- "A short hypoosmotic swelling test for the prediction of boar sperm fertility", 56, pp.387~398 (2001)
- "Flow cytometry and sorting of mouse testis cells DNA and mitochondrial changes during spermatogenesis", 19, pp.304~312 (1995)
- "Increased sperm nuclear DNA damage in normospermic infertile men: a prospective study.", 78, pp.313~318 (2002)
- "Genetic damage in oligozoospermic patients detected by fluorescence in-situ hybridization, inverse restriction site mutation assay, sperm chromatin structure assay and the Comet assay.", 18, pp.1474~1480 (2003)
- "Effect of cryopreservation on bovine sperm organelle function and viability as determined by flow cytometry", 58, pp.786~793 (1998)
- "Importance of sperm-binding assays for fertility prognosis of porcine spermatozoa", 63, pp.470~484 (2005)
- "Specific organization of genes in relation to the sperm nuclear matrix", pp.20~25 (1990)
- "Protection of boar spermatozoa from cold shock damage by 2-hydroxypropyl-beta-cyclodextrin", 55, pp.615~627 (2001)
