해삼(Stichopus japonicus)의 microsatellite 유전자형 분석을 위한 multiplex PCR 시스템 개발
Development of a Multiplex PCR System for Microsatellite Genotyping of the Sea Cucumber Stichopus japonicus
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초록
A multiplex PCR system comprising 14 microsatellite markers was developed for genotyping analysis of the seacucumber Stichopus japonicus. A total of 286 samples were used to evaluate genetic polymorphisms and forensicparameters of the microsatellite loci. In a single PCR reaction, all 14 loci were uniformly amplified and a total of269 alleles were identified. The AJ19024 locus had the largest number of alleles (46), and its discriminatory powerand exclusion power were 0.99 and 0.76, respectively. The fewest alleles (8) were present at the Psj2575 locus,which provided the lowest discriminatory power (0.81) and exclusion power (0.20). The mean number of alleles,mean heterozygosity, mean discrimination power and mean exclusion power per locus were 19.21, 0.70, 0.93, and0.46, respectively. The combined matching probability for the 14 loci was 9.64×10-19, and the combined power ofexclusion was 0.999995. Thus, the forensic parameters evaluated in the present study demonstrated the utility of ourmultiplex PCR system for biological tracing methods, such as individual identification and paternity testing, in thesea cucumber.
Abstract
A multiplex PCR system comprising 14 microsatellite markers was developed for genotyping analysis of the seacucumber Stichopus japonicus. A total of 286 samples were used to evaluate genetic polymorphisms and forensicparameters of the microsatellite loci. In a single PCR reaction, all 14 loci were uniformly amplified and a total of269 alleles were identified. The AJ19024 locus had the largest number of alleles (46), and its discriminatory powerand exclusion power were 0.99 and 0.76, respectively. The fewest alleles (8) were present at the Psj2575 locus,which provided the lowest discriminatory power (0.81) and exclusion power (0.20). The mean number of alleles,mean heterozygosity, mean discrimination power and mean exclusion power per locus were 19.21, 0.70, 0.93, and0.46, respectively. The combined matching probability for the 14 loci was 9.64×10-19, and the combined power ofexclusion was 0.999995. Thus, the forensic parameters evaluated in the present study demonstrated the utility of ourmultiplex PCR system for biological tracing methods, such as individual identification and paternity testing, in thesea cucumber.
참고문헌 (24)
- I.A. Arif, "Interpretation of electrophoretograms of seven microsatellite loci to determine the genetic diversity of the Arabian Oryx", Genetics and Molecular Research, 9(1), pp.259~265 (2010), Genetics and Molecular Research[DOI]
- Bacher JW, "Development of a fluorescent multiplex assay for detection of MSI-High tumors", Dis Markers, 20, pp.237~250 (2004)
- Karin D. Berg, "Detection of Microsatellite Instability by Fluorescence Multiplex Polymerase Chain Reaction", The Journal of Molecular Diagnostics, 2(1), pp.20~28 (2000), Elsevier BV[DOI]
- Chen M, "Identification of forty-five gene-derived polymorphic microsatellite loci for the sea cucumber, Apostichopus japonicus", J Genet, 92, pp.31~35 (2013)
- "FSTAT, a program to estimate and test gene diversities and fixation indices. Version 2.9.3" (2001)[URL]
- Sun Wei Guo, "Performing the Exact Test of Hardy-Weinberg Proportion for Multiple Alleles", Biometrics, 48(2), pp.361~ (1992), JSTOR[DOI]
- Sun Wei Guo, "Performing the Exact Test of Hardy-Weinberg Proportion for Multiple Alleles", Biometrics, 48(2), pp.361~ (1992), JSTOR[DOI]
- Hill CR, "A 26 plex autosomal STR assay to aid human identity testing", J Forensic Sci, 54, pp.1008~1015 (2009)
- Ivanova NV, "An inexpensive, automation-friendly protocol for recovering high-quality DNA", Mol Ecol Notes, 6, pp.998~1002 (2006)
- Jeffreys AJ, "Hypervariable minisatellite regions in human DNA", Nature, 314, pp.67~73 (1985)
- D.A. Jones, "Blood Samples : Probability of Discrimination", Journal of the Forensic Science Society, 12(2), pp.355~359 (1972), Elsevier BV[DOI]
- Kang JH, "Genetic differentiation among populations and color variants of sea cucumbers(Stichopus japonicus)from Korea and China", Int J Biol Sci, 30, pp.323~332 (2011)
- Manami Kanno, "Isolation and Characterization of Twenty Microsatellite Loci in Japanese Sea Cucumber (Stichopus japonicus)", Marine Biotechnology, 7(3), pp.179~183 (2005), Springer Nature[DOI]
- "Incheon Customs Press Release" (2016)[URL]
- Kim MJ, "Population genetic structure of sea cucumber, Stichopus japonicus in Korea using microsatellite markers", Aquaculture Res, 39, pp.1038~1045 (2008)
- Krenke BE, "Validation of a 16-locus fluorescent multiplex system", J Forensic Sci, 47, pp.773~777 (2002)
- J. Krger, "Zur Verwendung des Polymorphismus der sauren Erythrocytenphosphatase bei der Vaterschaftsbegutachtung", Deutsche Zeitschrift fr die Gesamte Gerichtliche Medizin, 64(2), pp.127~146 (1968), Springer Nature[DOI]
- 박광재, "한국 서해안 돌기해삼(Stichopus japonicus)의 서식지 기질 특성", 한국수산과학회지, 46(6), pp.886~891 (2013), 한국수산과학회
- "Holothuroidea. World Register of Marine Species" (2014)[URL]
- Purcell SW, "Ecological roles of exploited sea cucumbers. Oceanography and Marine Biology", An Annual Review, 54, pp.367~386 (2016)
- Tereba A., "Tools for analysis of population statistics, Profiles in DNA, vol 2", pp.14~16 (1999), Promega Corporation Press
- Stefania Turrina, "Evaluation of genetic parameters of 22 autosomal STR loci (PowerPlex® Fusion System) in a population sample from Northern Italy", International Journal of Legal Medicine, 128(2), pp.281~283 (2014), Springer Nature[DOI]
- Walsh PS, "Sequence analysis and characterization of stutter products at the tetranucleotide repeat locus vWA", Nucleic Acids Res, 24, pp.2807~2812 (1996)
- Zhan AB, "Development and characterization of 45 novel microsatellite markers for sea cucumber(Apostichopus japonicus)", Mol Ecol Notes, 7, pp.1345~1348 (2007)