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넙치(Paralichthys olivaceus)의 Streptococcus parauberis 인위감염을 위한 공격실험 방법에 관한 연구

An Experimental Study on Artificial Infection of Olive Flounder Paralichthys olivaceus by Streptococcus parauberis Using Different Injection Sites

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초록

Streptococcosis in the olive flounder Paralichthys olivaceus can be caused by Streptococcus parauberis. We compared three routes of administration for experimental injections of the S. parauberis 19FBSPa0003 strain in the olive flounder. Pathological changes were observed during the experimental infection. Inflammation of the serous membrane in the liver, intestine, spleen and heart was the major pathological change found in the infected olive flounder. No mortality was observed in fish that received intraperitoneal (IP) injection at less than 1×104 colony-forming unit (CFU)/fish. The lethal dose 50 for olive flounder, given an intravenous (IV) injection, was 7.94×104 CFU/fish. Fish with a higher concentration of IV injected S. parauberis (1×108 CFU/fish) died within a maximum of two days. However, serious necrosis and bacterial proliferation in ellipsoidal cells of the spleen and heart tissues were found in moribund or dead fish, 1-2 days after IV injection. Similar histopathological signs were observed in olive flounder inoculated by subcutaneous (SC) infected and naturally infected. In addition, SC was also strongly associated with bacteria concentration and cumulative mortality rate. Based on these results, SC is the recommended method for artificial infection by S. parauberis in the olive flounder.

Abstract

Streptococcosis in the olive flounder Paralichthys olivaceus can be caused by Streptococcus parauberis. We compared three routes of administration for experimental injections of the S. parauberis 19FBSPa0003 strain in the olive flounder. Pathological changes were observed during the experimental infection. Inflammation of the serous membrane in the liver, intestine, spleen and heart was the major pathological change found in the infected olive flounder. No mortality was observed in fish that received intraperitoneal (IP) injection at less than 1×104 colony-forming unit (CFU)/fish. The lethal dose 50 for olive flounder, given an intravenous (IV) injection, was 7.94×104 CFU/fish. Fish with a higher concentration of IV injected S. parauberis (1×108 CFU/fish) died within a maximum of two days. However, serious necrosis and bacterial proliferation in ellipsoidal cells of the spleen and heart tissues were found in moribund or dead fish, 1-2 days after IV injection. Similar histopathological signs were observed in olive flounder inoculated by subcutaneous (SC) infected and naturally infected. In addition, SC was also strongly associated with bacteria concentration and cumulative mortality rate. Based on these results, SC is the recommended method for artificial infection by S. parauberis in the olive flounder.

참고문헌 (38)

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관련 논문AI추천

양식 넙치, Paralichthys olivaceus에서 분리한 연쇄구균증 원인균, Streptococcus parauberis의 병원성권문경, 김진우, 이덕찬, 이재일, 조미영, 최동림, 최희정, 한명철수산생명의학회 · 한국어병학회지2009 · 제22권 제3호Streptococcus parauberis 인위 감염에 의한 넙치, Paralichthys olivaceus 심장의 병리학적 변화김병관, 김진우, 박명애, 원경미, 조미영, 최희정, 한명철수산생명의학회 · 한국어병학회지2009 · 제22권 제3호양식 강도다리, Platichthys stellatus Pallas에서 분리된 Streptococcus parauberis의 특성김명석, 김진우, 이덕찬, 이재일, 조미영수산생명의학회 · 한국어병학회지2008 · 제21권 제3호넙치, Paralichthys olivaceus 병어에서 분리된 Streptococcus parauberis의 면역 저항 특성김수현, 박수일, 우승호, 정영은, 하수진, 황성돈수산생명의학회 · 한국어병학회지2008 · 제21권 제3호Efficacy of a vaccine against Streptococcus parauberis infection in starry flounder Platichthys stellatus Pallas김도형, 김진우, 이덕찬, 이재일, 조미영수산생명의학회 · 한국어병학회지2011 · 제24권 제3호Application of tylosin antibiotics to olive flounder (Paralichthys olivaceus) infected with Streptococcus parauberis권문경, 김윤재, 박찬일, 서정수, 이지훈, 정지민, 주민수, 황성돈, 황지연, Hee-Chung Lee, Kwang-Min Choi한국수산과학회 · Fisheries and Aquatic Sciences2020 · 제23권 제3호

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