제주지역 전통발효식품에서 분리한 유산균의 기능적 특성
Functional Characteristics of Lactic Acid Bacteria Isolated From Jeju Island Traditional Fermented Food
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Jeju island is the largest island of South Korea, and has a relatively warm climate due to its geographical characteristics. These features have fostered development of various fermented foods distinct to Jeju island. Therefore, the functional activity of the lactic acid bacteria (LAB) isolated from the Jeju region's traditional fermented foods, including Kimchi and Jeotgal was characterized in this study. Fifteen strains were isolated from 6 types of fermented food. These strains include Enterococcus spp., Lactoplantibacillus spp., and Weisella spp. Several experiments were carried out to assess functional characteristics including acid resistance, bile resistance, hemolysis, DPPH and ABTS radical scavenging activity, and cholesterol-lowering activity. Three isolated strains (J-4, J-6 and J-10) exhibited high acid resistance, while the other 3 isolates (J-1, J-4 and J-6) showed high bile salt resistance. The strains displayed varied DPPH and ABTS radical scavenging activity: 71.14-86.42% among the first 3 strains, and 50.11-75.11% among the remaining 3 strains. Cholesterol-lowering activities were in the range 15.74-82.90% for all isolated strains. In conclusion, these studies suggest that isolated LAB strains from Jeju island traditional fermented foods possess the potential for broader application in the food industry.
Abstract
Jeju island is the largest island of South Korea, and has a relatively warm climate due to its geographical characteristics. These features have fostered development of various fermented foods distinct to Jeju island. Therefore, the functional activity of the lactic acid bacteria (LAB) isolated from the Jeju region's traditional fermented foods, including Kimchi and Jeotgal was characterized in this study. Fifteen strains were isolated from 6 types of fermented food. These strains include Enterococcus spp., Lactoplantibacillus spp., and Weisella spp. Several experiments were carried out to assess functional characteristics including acid resistance, bile resistance, hemolysis, DPPH and ABTS radical scavenging activity, and cholesterol-lowering activity. Three isolated strains (J-4, J-6 and J-10) exhibited high acid resistance, while the other 3 isolates (J-1, J-4 and J-6) showed high bile salt resistance. The strains displayed varied DPPH and ABTS radical scavenging activity: 71.14-86.42% among the first 3 strains, and 50.11-75.11% among the remaining 3 strains. Cholesterol-lowering activities were in the range 15.74-82.90% for all isolated strains. In conclusion, these studies suggest that isolated LAB strains from Jeju island traditional fermented foods possess the potential for broader application in the food industry.
참고문헌 (42)
- H. Şanlıdere Aloğlu, "Determination of antioxidant activity of bioactive peptide fractions obtained from yogurt", Journal of Dairy Science, 94(11), pp.5305~5314 (2011), American Dairy Science Association[DOI]
- Anthoula A. Argyri, "Selection of potential probiotic lactic acid bacteria from fermented olives by in vitro tests", Food Microbiology, 33(2), pp.282~291 (2013), Elsevier BV[DOI]
- Mutamed Ayyash, "In-vitro investigation into probiotic characterisation of Streptococcus and Enterococcus isolated from camel milk", LWT, 87, pp.478~487 (2018), Elsevier BV[DOI]
- 차인태, "발효식품의 마이크로바이옴 분석 기술", 식품과학과 산업, 50(1), pp.2~10 (2017), 한국식품과학회
- 차용준, "김장김치 담금시 부재료 특성 및 지역별 기호도 조사", 한국식품영양과학회지, 32(4), pp.555~561 (2003), 한국식품영양과학회
- Zhen-Yu Chen, "Role and classification of cholesterol-lowering functional foods", Journal of Functional Foods, 3(2), pp.61~69 (2011), Elsevier BV[DOI]
- Jinhee Cho, "Microbial population dynamics of kimchi, a fermented cabbage product", FEMS Microbiology Letters, 257(2), pp.262~267 (2006), Oxford University Press (OUP)[DOI]
- 조진국, "김치로부터 분리한 Lactobacillus plantarum JK-01의 동정 및 생리적 특성", 한국축산식품학회지, 27(3), pp.363~370 (2007), 한국축산식품학회
- 최하늘, "김치 분리 유산균의 치즈 곰팡이 항진균 활성", Food Science and Preservation, 20(5), pp.727~734 (2013), 한국식품저장유통학회
- K.H. Sabeena Farvin, "Antioxidant activity of yoghurt peptides: Part 2 – Characterisation of peptide fractions", Food Chemistry, 123(4), pp.1090~1097 (2010), Elsevier BV[DOI]
- Joseph Felsenstein, "CONFIDENCE LIMITS ON PHYLOGENIES: AN APPROACH USING THE BOOTSTRAP", Evolution, 39(4), pp.783~791 (1985), Wiley[DOI]
- Debapriya Hazra, "Generating Synthetic Fermentation Data of Shindari, a Traditional Jeju Beverage, Using Multiple Imputation Ensemble and Generative Adversarial Networks", Applied Sciences, 11(6), pp.2787~ (2021), MDPI AG[DOI]
- Ching-Chiang Hwang, "Chromatographic characteristics of cholesterol-imprinted polymers prepared by covalent and non-covalent imprinting methods", Journal of Chromatography A, 962(1-2), pp.69~78 (2002), Elsevier BV[DOI]
- Jeun JH, "Isolation and identification of Lactobacillus sp. produced γ-aminobutyric acid(GABA)form traditional slat fermented anchovy", J Korean Soc Food Sci Nutr, 17, pp.72~79 (2004)
- 정상은, "시판 생막걸리에서 분리한 유산균의 프로바이오틱스 기능성 연구", 한국식품과학회지, 47(1), pp.44~50 (2015), 한국식품과학회[DOI]
- Khalkhali S, "Bacteriocinogenic potential and virulence traits of Enterococcus faecium and E. faecalis isolated from human milk", Iran J Microbiol, 9, pp.224~233 (2017)
- 김다영, "한국전통식품 김치로부터 분리한 유산균주의 항산화 활성", Journal of Dairy Science and Biotechnology, 38(2), pp.89~97 (2020), 한국낙농식품응용생물학회[DOI]
- Kim JH, "Inhibition of Listeria monocytogenes by bacteriocin(s)from lactic acid bacteria isolated from Kimchi", Appl Biol Chem, 38, pp.302~307 (1995)
- 김진학, "전통식품 유래 유산균의 해조류 발효 및 Probiotic 특성", 한국식품영양과학회지, 45(10), pp.1481~1487 (2016), 한국식품영양과학회
- Min-Soo Kim, "Bacterial Communities of Traditional Salted and Fermented Seafoods from Jeju Island of Korea Using 16S rRNA Gene Clone Library Analysis", Journal of Food Science, 79(5), pp.M927~M934 (2014), Wiley[DOI]
- 고강희, "김치 종류에 따른 유산균의 생물학적 및 기능적 특성", 한국식품영양과학회지, 42(1), pp.89~95 (2013), 한국식품영양과학회
- 국무창, "전통 발효식품으로부터 Protease 활성을 보유한 유산균의 분리 및 동정", 산업식품공학, 15(2), pp.182~187 (2011), 한국산업식품공학회
- Kumar S, "MEGA X : molecular evolutionary genetics analysis across computing platforms", Mol Biol Evol, 35, pp.1547~1549 (2018)[DOI]
- Bao Le, "Efficacy of Lactobacillus plantarum in prevention of inflammatory bowel disease", Toxicology Reports, 5, pp.314~317 (2018), Elsevier BV[DOI]
- 이윤주, "Identification of Lactic Acid Bacteria in Galchi- and Myeolchi-Jeotgal by 16S rRNA Gene Sequencing, MALDI-TOF Mass Spectrometry, and PCR-DGGE", Journal of Microbiology and Biotechnology, 28(7), pp.1112~1121 (2018), 한국미생물·생명공학회
- 임성미, "멸치 젓갈로부터 분리된 젖산세균의 프로바이오틱 특성 및 안전성 평가", 한국식품과학회지, 48(4), pp.306~316 (2016), 한국식품과학회[DOI]
- H.-S. Lye, "Removal of cholesterol by lactobacilli via incorporation and conversion to coprostanol", Journal of Dairy Science, 93(4), pp.1383~1392 (2010), American Dairy Science Association[DOI]
- Nei M, "Molecular evolution and phylogenetics" (2000), Oxford University Press
- 오유성, "제주전통된장으로부터 세포외효소 분비능이 우수한 미생물의 분리 및 특성", 한국식품영양과학회지, 39(1), pp.47~53 (2010), 한국식품영양과학회
- 박홍엽, "김치에서 분리된 콜레스테롤 감소능을 가진 젖산세균의 특성", 한국식품과학회지, 49(4), pp.377~382 (2017), 한국식품과학회[DOI]
- 박종혁, "전통발효식품에서 분리한 유산균의 항균 활성 및 발효유 스타터 개발", Food Science and Preservation, 20(5), pp.712~719 (2013), 한국식품저장유통학회
- 박정민, "Identification of the Lactic Acid Bacteria in Kimchi According to Initial and Over-ripened Fermentation Using PCR and 16S rRNA Gene Sequence Analysis", Food Science and Biotechnology, 19(2), pp.541~546 (2010), 한국식품과학회
- Soo-Yeong Park, "홍해삼 첨가 김치의 저장기간 중 품질 특성 및 기호도 변화", Journal of Applied Biological Chemistry, 55(2), pp.135~140 (2012), 한국응용생명화학회[DOI]
- 박연진, "항균활성 김치유산균의 분리 및 그 특성 규명", 한국생활과학회지, 26(6), pp.547~558 (2017), 한국생활과학회[DOI]
- Anil K. Patel, "Probiotic Bile Salt Hydrolase: Current Developments and Perspectives", Applied Biochemistry and Biotechnology, 162(1), pp.166~180 (2010), Springer Science and Business Media LLC[DOI]
- 류대규, "다시마(Saccharina japonica)김치에서 분리한 유산균의 항산화 및 콜레스테롤 감소 효과", 한국수산과학회지, 53(3), pp.351~360 (2020), 한국수산과학회[DOI]
- Eun Hye Ryu, "In vitro study of potentially probiotic lactic acid bacteria strains isolated from kimchi", Annals of Microbiology, 63(4), pp.1387~1395 (2013), Springer Science and Business Media LLC[DOI]
- 유영수, "제주 전통 발효식품 쉰다리에서 분리한 세균의 군집 조사 및 어류질병세균과 인체유해세균에 대한 항균활성효과", 생명과학회지, 31(1), pp.73~82 (2021), 한국생명과학회[DOI]
- Sahadeva RPK, "Survival of commercial probiotic strains to pH and bile", Int Food Res, 18, pp.1515~1522 (2011)
- Saitou N, "The neighbor-joining method: a new method for reconstructing phylogenetic trees", Molecular Biology and Evolution, 4, pp.406~425 (1987), Oxford University Press (OUP)[DOI]
- 서재훈, "Probiotics로서의 젖산균주의 특성 및 면역활성", 한국식품과학회지, 39(6), pp.681~687 (2007), 한국식품과학회
- Hyung-Seok Yu, "Evaluation of the probiotic characteristics and prophylactic potential of Weissella cibaria strains isolated from kimchi", LWT, 112, pp.108229~ (2019), Elsevier BV[DOI]
