방사선 돌연변이 육종기술을 통한 고 아미노산 함유 김(Pyropia yezoensis) 돌연변이 개발
Development of Pyropia yezoensis Mutant with Improved Amino Acid Content Using Gamma Rays
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Gamma irradiation is one of the simple methods used to induce mutagenesis. Therefore, it is widely used for the development of breeding lineages of plants and algae. In this study, it was developed a new variety of Pyropia yezoensis using gamma irradiation. It was applied a dose of 1 kGy and named the developed mutant Py1k. The blade with width of the mutant was narrower and the blade length was longer than those of the wild type. To further investigate the mutant, it was analyzed the nutrient composition and antioxidant activity. In comparison to those in the wild type, it was found a higher amino acid composition and marginally increased antioxidant activity in Py1k. Based on these results, it was suggests that our protocol can be utilized to develop Pyropia species with improved nutritional quality through gamma irradiation.
Abstract
Gamma irradiation is one of the simple methods used to induce mutagenesis. Therefore, it is widely used for the development of breeding lineages of plants and algae. In this study, it was developed a new variety of Pyropia yezoensis using gamma irradiation. It was applied a dose of 1 kGy and named the developed mutant Py1k. The blade with width of the mutant was narrower and the blade length was longer than those of the wild type. To further investigate the mutant, it was analyzed the nutrient composition and antioxidant activity. In comparison to those in the wild type, it was found a higher amino acid composition and marginally increased antioxidant activity in Py1k. Based on these results, it was suggests that our protocol can be utilized to develop Pyropia species with improved nutritional quality through gamma irradiation.
참고문헌 (48)
- 백승연, "한국산 방사무늬김과 잇바디돌김의 영양성분, Phytochemicals 및 항산화 활성 비교", 한국식품영양과학회지, 48(11), pp.1233~1243 (2019), 한국식품영양과학회
- Iris F.F. Benzie, "The Ferric Reducing Ability of Plasma (FRAP) as a Measure of “Antioxidant Power”: The FRAP Assay", Analytical Biochemistry, 239(1), pp.70~76 (1996), Elsevier BV[DOI]
- Sung‐Hwan Cho, "The ricenarrow leaf2andnarrow leaf3loci encodeWUSCHEL‐related homeobox 3A(OsWOX3A) and function in leaf, spikelet, tiller and lateral root development", New Phytologist, 198(4), pp.1071~1084 (2013), Wiley[DOI]
- 최종일, "방사선 돌연변이 방사무늬김(Pyropia yezoensis)의 성분 분석과 항산화 활성", 한국수산과학회지, 53(4), pp.524~529 (2020), 한국수산과학회[DOI]
- Christine Dawczynski, "Amino acids, fatty acids, and dietary fibre in edible seaweed products", Food Chemistry, 103(3), pp.891~899 (2007), Elsevier BV[DOI]
- Veronica Dewanto, "Processed Sweet Corn Has Higher Antioxidant Activity", Journal of Agricultural and Food Chemistry, 50(17), pp.4959~4964 (2002), American Chemical Society (ACS)[DOI]
- Hongchang Ding, "Isolation and characterization of a heat-resistant strain with high yield of Pyropia yezoensis Ueda (Bangiales, Rhodophyta)", Aquaculture and Fisheries, 1, pp.24~33 (2016), Elsevier BV[DOI]
- J. Allan Feurtado, "TheArabidopsisC2H2 Zinc Finger INDETERMINATE DOMAIN1", ENHYDROUS Promotes the Transition to Germination by Regulating Light and Hormonal Signaling during Seed Maturation, 2011, pp.23(5), 1772~1794[DOI]
- Peter Fürst, "What Are the Essential Elements Needed for the Determination of Amino Acid Requirements in Humans?", The Journal of Nutrition, 134(6), pp.1558S~1565S (2004), Oxford University Press (OUP)[DOI]
- Jitender Giri, "Rice A20", AN1 zinc‐finger containing stress‐associated proteins (SAP1, New Phytologist, pp.2011, 191(3)[DOI]
- Eun Kyoung Hwang, "Seaweed breeding programs and progress in eastern Asian countries", Phycologia, 58(5), pp.484~495 (2019), Informa UK Limited[DOI]
- 황미숙, "참김 (Porphyra tenera Kjellman) 색소 및 형태변이체의 순계주 분리 및 특성", 한국수산과학회지, 43(5), pp.495~502 (2010), 한국수산과학회
- Nao INOUE, "The Sulfated Polysaccharide Porphyran Reduces Apolipoprotein B100 Secretion and Lipid Synthesis in HepG2 Cells", Bioscience, Biotechnology, and Biochemistry, 73(2), pp.447~449 (2009), Oxford University Press (OUP)[DOI]
- Heng Jiang, "Effects of seawater acidification and alkalization on the farmed seaweed, Pyropia haitanensis (Bangiales, Rhodophyta), grown under different irradiance conditions", Algal Research, 31, pp.413~420 (2018), Elsevier BV[DOI]
- Jimenez-Escrig A, "Nutritional evaluation and physiological effects of edinle seaweeds", Arch Latinoam Nutr, 49, pp.114~120 (1999)
- 정상목, "The Analysis of Proximate Composition, Minerals and Amino Acid Content of Red Alga Pyropia dentata by Cultivation Sites", 한국환경생태학회지, 29(1), pp.01~06 (2015), 한국환경생태학회
- 정효정, "국내산 김(Porphyra tenera), 다시마(Saccharina japonicus), 미역(Undaria pinnatifida), 톳(Sargassum fusiforme) 의 생산지역별 따른 미네랄 함량 분석 및 영양평가", 수산해양교육연구, 29(5), pp.1624~1632 (2017), 한국수산해양교육학회[DOI]
- 김훈, "항산화제 Quercetin이 파라쿼트에 유도되는 세포 손상에 미치는 효과", 대한응급의학회지, 18(1), pp.41~47 (2007), 대한응급의학회
- 김기웅, "시판 건조김의 주요 영양성분", Food Science and Preservation, 21(5), pp.702~709 (2014), 한국식품저장유통학회
- "Fisheries statistics" (2020)[URL]
- 곽연화, "해조류 NDF에 결합된 주요 무기질(Ca, P, Mg)의 함량 분석 및 추정 이용률", 한국식품영양과학회지, 39(7), pp.1073~1077 (2010), 한국식품영양과학회
- Mi-Jin Kwon, "Porphyran induces apoptosis related signal pathway in AGS gastric cancer cell lines", Life Sciences, 79(20), pp.1956~1962 (2006), Elsevier BV[DOI]
- 이학증, "국내산 마른 김(Porphyra )의 생리활성 및 아미노산 조성 비교", 한국수산과학회지, 45(5), pp.409~413 (2012), 한국수산과학회
- 이학정, "Isolation, Morphological Characteristics and Proteomic Profile Analysis of Thermo-tolerant Pyropia yezoensis Mutant in Response to High-temperature Stress", Ocean Science Journal, 54(1), pp.65~78 (2019), 한국해양과학기술원[DOI]
- Lee KH, "Quality changes of dried lavers during processing and storage. 1. Quality evaluation of different grades of dried lavers and its changes during storage", J Kor Fish Soc, 20, pp.408~418 (1987)
- Ying Xia Li, "Induction and characterization of green pigmentation mutant in Porphyra yezoensis Ueda", Aquaculture, 282(1-4), pp.117~123 (2008), Elsevier BV[DOI]
- S. N. Lim, "Evaluation of Antioxidative Activity of Extracts from a Brown Seaweed, Sargassum siliquastrum", Journal of Agricultural and Food Chemistry, 50(13), pp.3862~3866 (2002), American Chemical Society (ACS)[DOI]
- Yingchao Ma, "Characterization of a high-growth-rate mutant strain of Pyropia yezoensis using physiology measurement and transcriptome analysis", Journal of Phycology, 55(3), pp.651~662 (2019), Wiley[DOI]
- Ole G. Mouritsen, "Umami taste, free amino acid composition, and volatile compounds of brown seaweeds", Journal of Applied Phycology, 31(2), pp.1213~1232 (2019), Springer Science and Business Media LLC[DOI]
- Miyuki Nakata, "Roles of the Middle Domain–Specific WUSCHEL-RELATED HOMEOBOX Genes in Early Development of Leaves in Arabidopsis", The Plant Cell, 24(2), pp.519~535 (2012), Oxford University Press (OUP)[DOI]
- Judith Nardmann, "The maize duplicate genes narrow sheath1 and narrow sheath2 encode a conserved homeobox gene function in a lateral domain of shoot apical meristems", Development, 131(12), pp.2827~2839 (2004), The Company of Biologists[DOI]
- Kyosuke Niwa, "Induction and isolation of pigmentation mutants of Porphyra yezoensis (Bangiales, Rhodophyta) by heavy-ion beam irradiation", Phycological Research, 57(3), pp.194~202 (2009), Wiley[DOI]
- Kyosuke Niwa, "Mutation breeding in the marine crop Porphyra yezoensis (Bangiales, Rhodophyta): Cultivation experiment of the artificial red mutant isolated by heavy-ion beam mutagenesis", Aquaculture, 314(1-4), pp.182~187 (2011), Elsevier BV[DOI]
- Park CK, "Extractive nitrogenous constituents of dried laver Porphyra yezoensis", Korean J Fish Soc, 34, pp.394~402 (2001)
- 박은정, "Resistance and Proteomic Response of Microalgae to Ionizing Irradiation", Biotechnology and Bioprocess Engineering, 23(6), pp.704~709 (2018), 한국생물공학회
- Seo-jeong Park, "De novo transcriptome analysis of high growth rate Pyropia yezoensis (Bangiales, Rhodophyta) mutant with high utilization of nitrogen", Acta botanica Croatica, 79(2), pp.201~211 (2020), University of Zagreb, Faculty of Science, Department of Biology[DOI]
- 박욱민, "시판 건조김의 유기산, 비타민 및 유리당 함량", 한국식품영양과학회지, 43(1), pp.172~177 (2014), 한국식품영양과학회
- C. Ruiz-Capillas, "Changes in free amino acids during chilled storage of hake ( Merluccius merluccius L. ) in controlled atmospheres and their use as a quality control index", European Food Research and Technology, 212(3), pp.302~307 (2001), Springer Science and Business Media LLC[DOI]
- 신동민, "채취시기별 방사무늬김(Porphyra yezoensis)의 식이섬유, 아미노산 및 지방산 함량 변화", 한국수산과학회지, 46(4), pp.337~342 (2013), 한국수산과학회
- 신윤주, "Characterization of high temperature-tolerant strains of Pyropia yezoensis", Plant Biotechnology Reports, 12(5), pp.365~373 (2018), 한국식물생명공학회
- Keisuke TSUGE, "Dietary Effects of Porphyran from Porphyra yezoensis on Growth and Lipid Metabolism of Sprague-Dawley Rats", Food Science and Technology Research, 10(2), pp.147~151 (2004), Japanese Society for Food Science and Technology[DOI]
- Ellen J. Van Loo, "Healthy, sustainable and plant-based eating: Perceived (mis)match and involvement-based consumer segments as targets for future policy", Food Policy, 69, pp.46~57 (2017), Elsevier BV[DOI]
- Michiel Vandenbussche, "Differential Recruitment ofWOXTranscription Factors for Lateral Development and Organ Fusion in Petunia andArabidopsis", The Plant Cell, 21(8), pp.2269~2283 (2009), Oxford University Press (OUP)[DOI]
- Watanabe K, "Meat flavor", Japan J Zootech Sci, 45, pp.113~128 (1974)
- Yan XH, "Induction and characterization of pigmentation mutants in Porphyra yezoensis(Bangiales, Rhodophyta)", J Appl Phycol, 12, pp.69~81 (2000)
- Yoshie Y, "Dietary fiber, minerals, free amino acids and fatty acid compositions in dried Nori of several culture places in Korea", J Tokyo Univ Fish, 80, pp.197~203 (1993)
- Feng Zhao, "Microtubule-Mediated Wall Anisotropy Contributes to Leaf Blade Flattening", Current Biology, 30(20), pp.3972~3985.e6 (2020), Elsevier BV[DOI]
- Zhongjuan Zhang, "A WOX", Auxin Biosynthesis Module Controls Growth to Shape Leaf Form, 2020, pp.30(24), 4857~4868.e6[DOI]
