Optimization of a Diagnostic DNA Chip for Fish Rhabdovirus
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Abstract
A DNA chip that rapidly and accurately detects the viral genes in rhabdovirus-infected fish was developed. The N, M1, and G proteins of three rhabdovirus strains, infectious hematopoietic necrosis virus (IHNV), viral hemorrhagic septicemia virus (VHSV), and flounder rhabdovirus (HIRRV), were selected for use as probes. The sequences of the corresponding genes were obtained, and probes were prepared by PCR using specific primer sets. The specificity of the probes was confirmed by cross PCR. The prepared probes were spotted on poly-L-lysine- or aminosilane-coated glass slides and hybridized with target DNA under several different conditions in order to determine the optimal hybridization temperature, glass-slide coating, and target cDNA concentration.
참고문헌 (10)
- "DNA Microarrays", pp.79~256 (2003), Cold Spring Habor Laboratory Press
- "Diffe-rential diagnosis of fish pathogenic rhabdoviruses by reverse transcriptase-dependent polymerase chain reaction", 55, pp.111~119 (1995)
- "Identification of cDNA for medaka cytoskeletal β-actin and construc-tion for reverse transcriptase-polymerase chain reac-tion", 63, pp.73~76 (1997)
- "Infection of viral hemorrhagic septicemia virus(VHSV) in cultured olive flounder, Paralichtyys olivaceus." (2003), Pukyong National University
- "Rapid and sensitive reverse trans-criptase-polymerase chain reaction based detection and differential diagnosis of fish pathogenic rhabdo-virus in organ samples and cultured cells", 34, pp.13~20 (1998)
- "Sequence analysis of the 3' half of the hirame rhabdovirus(HIRRV) RNA genome", (me) (1999), Pukyong National University
- "Microarray Analys", pp.1~25 (2003), Wiley-Liss Book Co
- "Complete genomic sequence of viral hemorrhagic septicemia virus hemorrhagic septicemia virus, a fish rhabdivirus", 19, pp.59~65 (1999)
- "increa-sing spot accuracy and automated identification of true microarray signals", e54. (2002)
- "Comparison between different strategies of covalent attachment of DNA to glass surfaces to build DNA microarrays", 280, pp.143~150 (2000)