해조류 방사무늬김 (Porphyra yezoensis) 엽체로부터 산 유도 유전자의 분리
Differential Display Detection of Acid-inducible Genes from Porphyra yezoensis Thalli
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Abstract
Genetic responses of the edible seaweed Porphyra yezoensis tissue to acid shock have been compared using differential display technique. The tissue was challenged in seawater containing 0.05% hydrogen chloride (pH 3.0) for 5min, then rehabilitated in normal seawater for 10min, 30min, 60min and 4hrs. Total RNA extracted by the LiCl-guanidium method was reverse transcribed and amplified by PCR with arbitrary primers. The amplified fragment responded by the acid shock was selectively isolated from agarose gel and sequenced with DNA auto sequencer. Sequence (1056bp) of the cDNA contained at least two genes for ASP7K (MW 7418) and ASP5K (MW 5512) proteins.
참고문헌 (14)
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