Potential of watermelon (Citrullus lanatus) to maintain oxidative stability of rooster semen for artificial insemination
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초록
Fruits with antioxidant enrichment can be an economically affordable supplement for mitigating oxidative damage prone spermatozoa membrane pathologies. Computer-assisted sperm analyzer and oxidative status were utilized to evaluate the impact of watermelon (Citrullus lanatus) fortification of dextrose saline as diluent for rooster semen and fertility response of hens inseminated. Watermelon juice and dextrose saline were used to formulate diluent of 7 treatments consisting of unextended semen (positive control), 10%, 20%, 30%, 40%, 50% and only dextrose saline (negative control) designated as Treatments 1–7. Pooled semen was obtained from fertile roosters and equilibrated with diluents at ratio 1:2 in the various treatments and were evaluated using computer software coupled microscope and seminal oxidative status assay. 168 laying hens randomly divided into 7 treatment of 8 replicates and 3 hen per replicate. Hen were everted, and semen (2 × 108 Spermatozoa) deposited intra-vagina and eggs collected over 8 weeks to assess fertility and hatchability of eggs laid. The result obtained revealed that watermelon-dextrose saline rooster semen diluent enhanced progressive motility, sperm kinetics and lowered non-progressive motility in T2–T6 compared to T7 over the 3 hours of evaluation. Watermelon addition to rooster semen diluent enhance the antioxidant capacity of rooster semen and lowered lipid peroxide generation. The percentage fertility was highest in T3 (81.01%) and T4 (81.24%) with lowest value obtained in T7 (73.46%). The hatchability of eggs set of hens inseminated with undiluted semen (71.46%) was lower than values for hens inseminated with watermelon inclusive extended semen (75.71%–80.39%). The optimal inclusion of 30%–40% watermelon in dextrose saline diluent enhance rooster semen kinetics, seminal oxidative stability and egg fertility.
Abstract
Fruits with antioxidant enrichment can be an economically affordable supplement for mitigating oxidative damage prone spermatozoa membrane pathologies. Computer-assisted sperm analyzer and oxidative status were utilized to evaluate the impact of watermelon (Citrullus lanatus) fortification of dextrose saline as diluent for rooster semen and fertility response of hens inseminated. Watermelon juice and dextrose saline were used to formulate diluent of 7 treatments consisting of unextended semen (positive control), 10%, 20%, 30%, 40%, 50% and only dextrose saline (negative control) designated as Treatments 1–7. Pooled semen was obtained from fertile roosters and equilibrated with diluents at ratio 1:2 in the various treatments and were evaluated using computer software coupled microscope and seminal oxidative status assay. 168 laying hens randomly divided into 7 treatment of 8 replicates and 3 hen per replicate. Hen were everted, and semen (2 × 108 Spermatozoa) deposited intra-vagina and eggs collected over 8 weeks to assess fertility and hatchability of eggs laid. The result obtained revealed that watermelon-dextrose saline rooster semen diluent enhanced progressive motility, sperm kinetics and lowered non-progressive motility in T2–T6 compared to T7 over the 3 hours of evaluation. Watermelon addition to rooster semen diluent enhance the antioxidant capacity of rooster semen and lowered lipid peroxide generation. The percentage fertility was highest in T3 (81.01%) and T4 (81.24%) with lowest value obtained in T7 (73.46%). The hatchability of eggs set of hens inseminated with undiluted semen (71.46%) was lower than values for hens inseminated with watermelon inclusive extended semen (75.71%–80.39%). The optimal inclusion of 30%–40% watermelon in dextrose saline diluent enhance rooster semen kinetics, seminal oxidative stability and egg fertility.
참고문헌 (25)
- Esguerra JPM, "Coconut water with either tomato juice or garlic extract as extender components for Paraoakan native chicken semen at different storage temperatures", Philipp J Sci, 149, pp.121~131 (2020)
- Aji R, "The effect of Andromed® and coconut water+ 20% egg yolk as diluent on semen motility of Belgian Blue cattle", IOP Conf Ser Earth and Environ Sci, 387, pp.012127~ (2019)
- Jimoh OA, "Assessment of the oxidative stress markers and reproductive performance of four exotic breeds of rabbit in Ibadan", Ph.D., pp.2016
- Jimoh OA, "Semen characteristics, seminal biochemical and oxidative stress markers in rabbits during heat stress", J Vet Androl, 3, pp.35~44 (2018)
- Jimoh OA, "Antioxidative effect of mistletoe leaf meal supplemented diets in laying pullets", Arch de Zootec, 67, pp.526~530 (2018)[DOI]
- Olatunji Abubakar Jimoh, "Protective effect of soursop (Annona muricata linn.) juice on oxidative stress in heat stressed rabbits", 한국축산학회지, 60(11), pp.1~6 (2018), 한국축산학회[DOI]
- Jimoh OA, "Antioxidant status and serology of laying pullets fed diets supplemented with mistletoe leaf meal", Niger J Anim Sci, 20, pp.52~60 (2018)
- Bayemi PH, "Use of fruits and raffia palm sap (Raffia hookeri) in chilled bull semen extenders in Cameroon", Agric Biol Sci J, 1, pp.142~149 (2015)
- Balogun A, "Efficacy of egg-yolk citrate extender fortified with aqueous garlic extract on rooster semen for artificial insemination", Niger J Anim Sci, 19, pp.62~70 (2017)
- RedaIbrahim El-Sheshtawy, "Cryopreservation of cattle semen using coconut water extender with different glycerol concentrations", Asian Pacific Journal of Reproduction, 6(6), pp.279~282 (2017), Medknow[DOI]
- Khaki A, "Effects of watermelon seed extract (Citrullus vulgaris)on spermatogenesis in rat", Int J Women’s Health Reprod Sci, 1, pp.99~104 (2013)[DOI]
- Kolawole T, "Ameliorative effects of hydromethanolic extract of Citrullus lanatus (watermelon) rind on semen parameters, reproductive hormones and testicular oxidative status following nicotine administration in male Wistar rats", Niger J Physiol Sci, 34, pp.83~90 (2019)
- O.A. Jimoh, "Calidad y fertilidad del semen de conejo diluido con jugo de sandía", Arch de zootec, 69(266), pp.140~146 (2020), Cordoba University Press (UCOPress)[DOI]
- Balogun A, "Comparative semen characteristics of Nigeria indigenous cock and exotic strains of domestic fowl", Niger J Anim Sci, 17, pp.148~153 (2015)
- Hiroshi Ohkawa, "Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction", Analytical Biochemistry, 95(2), pp.351~358 (1979), Elsevier BV[DOI]
- D Koracevic, "Method for the measurement of antioxidant activity in human fluids", Journal of Clinical Pathology, 54(5), pp.356~361 (2001), BMJ[DOI]
- Adebisi K, "Comparative effect of saline solutions as diluents on in vitro semen storage, egg fertility and hatchability in Turkey hens", Niger J Anim Sci, 21, pp.107~116 (2019)
- Ezekiel Adekunle, "Effects of apple and orange juices on quality of refrigerated goat semen", Journal of Agricultural Sciences, Belgrade, 63(1), pp.53~65 (2018), National Library of Serbia[DOI]
- Reda I. El-Sheshtawy, "Effects of pomegranate juice in Tris-based extender on cattle semen quality after chilling and cryopreservation", Asian Pacific Journal of Reproduction, 5(4), pp.335~339 (2016), Asian Pacific Journal of Tropical Medicine Press[DOI]
- E. Tvrda, "Antioxidant effects of lycopene on bovine sperm survival and oxidative profile following cryopreservation", Veterinární Medicína, 62(8), pp.429~436 (2017), Czech Academy of Agricultural Sciences[DOI]
- Amedu NO, "Dietary consumption of citrullus lanatus can ameliorate infertility potential of carica papaya seeds extract in male rats", Biotechnol J Int, 11, pp.1~9 (2016)[DOI]
- Mangiagalli MG, "Effect of lycopene on fowl sperm characteristics during in vitro storage", Archiv für Geflügelkunde, 71, pp.25~29 (2007)
- Chetna Gangwar, "Effect of vitamin C supplementation on freezability of Barbari buck semen", Small Ruminant Research, 129, pp.104~107 (2015), Elsevier BV[DOI]
- Rong-Liang Zheng, "Effects of Ferulic Acid on Fertile and Asthenozoospermic Infertile Human Sperm Motility, Viability, Lipid Peroxidation, and Cyclic Nucleotides", Free Radical Biology and Medicine, 22(4), pp.581~586 (1997), Elsevier BV[DOI]
- M.G. Mangiagalli, "Effect of lycopene supplementation on semen quality and reproductive performance in rabbit", World Rabbit Science, 20(3), pp.141~148 (2012), Universitat Politecnica de Valencia[DOI]