Estrus synchronization and artificial insemination in Korean black goat (Capra hircus coreanae) using frozen-thawed semen
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초록
Presently, there is an increased demand for livestock products all over the world which has led to more devotion on improving livestock population. Although goats have been bred for a long time in Korea, but there is not much research conducted on traditional Korean black goat (Capra hircus coreanae) compared to other livestock populations. Mutton consumption has been dramatically changing from medicinal use to edible meat and this trend directs the black goat populations declining and also mutton import quantities are increasing consistently. The present study introduced a new estrus synchronizing technique with subsequent artificial insemination (AI) for Korean black goats to enable crossbreeding with non-native breeds for the small or subsistent farmers. Our data highlighted that, the percentage of motile sperm from the electro-ejaculated samples declined significantly after freezing and melting. In addition, the sperm motility significantly declined with regard to sperm incubation period (0, 5, 60, and 120 min at 37℃) and was negatively correlated (64.2 ± 7.9%, 63.3 ± 5.8%, 49.9 ± 6.3%, and 35.9 ± 7.6%, respectively) in frozen-thawed sperm samples. Moreover, the E2 levels were unchanged even 24 h after controlled internal drug releas (CIDR) withdrawal. But, 48 h and 72 h after CIDR removal, E2 levels increased significantly. These data helps us to consider the two time points for AI; CIDR removal after 24 h, at which E2 decreases, and after 48 h, as the time at which progesterone increases. Additionally, the AI after 48 h of CIDR removal group exhibited significantly higher pregnancy and parturition rates (42.9%) compared to AI after 24 h after CIDR removal 28.6% group. In conclusion, these studies will propose an optimal estrus synchronisation process with subsequent timing of AI and also will promote the Korean black goat breeding industry.
Abstract
Presently, there is an increased demand for livestock products all over the world which has led to more devotion on improving livestock population. Although goats have been bred for a long time in Korea, but there is not much research conducted on traditional Korean black goat (Capra hircus coreanae) compared to other livestock populations. Mutton consumption has been dramatically changing from medicinal use to edible meat and this trend directs the black goat populations declining and also mutton import quantities are increasing consistently. The present study introduced a new estrus synchronizing technique with subsequent artificial insemination (AI) for Korean black goats to enable crossbreeding with non-native breeds for the small or subsistent farmers. Our data highlighted that, the percentage of motile sperm from the electro-ejaculated samples declined significantly after freezing and melting. In addition, the sperm motility significantly declined with regard to sperm incubation period (0, 5, 60, and 120 min at 37℃) and was negatively correlated (64.2 ± 7.9%, 63.3 ± 5.8%, 49.9 ± 6.3%, and 35.9 ± 7.6%, respectively) in frozen-thawed sperm samples. Moreover, the E2 levels were unchanged even 24 h after controlled internal drug releas (CIDR) withdrawal. But, 48 h and 72 h after CIDR removal, E2 levels increased significantly. These data helps us to consider the two time points for AI; CIDR removal after 24 h, at which E2 decreases, and after 48 h, as the time at which progesterone increases. Additionally, the AI after 48 h of CIDR removal group exhibited significantly higher pregnancy and parturition rates (42.9%) compared to AI after 24 h after CIDR removal 28.6% group. In conclusion, these studies will propose an optimal estrus synchronisation process with subsequent timing of AI and also will promote the Korean black goat breeding industry.
참고문헌 (30)
- M.M.R. Chowdhury, "In vitro production of sex preselected cattle embryos using a monoclonal antibody raised against bull sperm epitopes", Animal Reproduction Science, 205, pp.156~164 (2019), Elsevier BV[DOI]
- Yongju Zhao, "Efficiency of methods applied for goat estrous synchronization in subtropical monsoonal climate zone of Southwest China", Tropical Animal Health and Production, 42(6), pp.1257~1262 (2010), Springer Science and Business Media LLC[DOI]
- Baldassarre H, "Advanced assisted reproduction technologies (ART) in goats", Anim Reprod Sci, 82-83, pp.255~266 (2004)[DOI]
- J.R. Pursley, "Synchronization of ovulation in dairy cows using PGF2α and GnRH", Theriogenology, 44(7), pp.915~923 (1995), Elsevier BV[DOI]
- W. Holtz, "Ovsynch synchronization and fixed-time insemination in goats", Theriogenology, 69(7), pp.785~792 (2008), Elsevier BV[DOI]
- B Leboeuf, "Production and storage of goat semen for artificial insemination", Animal Reproduction Science, 62(1-3), pp.113~141 (2000), Elsevier BV[DOI]
- Bogdan L, "Research concerning estrus induction and synchronization in anestrous (out of season) goats", Vet Med, 65, pp.91~95 (2008)[DOI]
- M.F. Martínez, "Effects of estradiol on gonadotrophin release, estrus and ovulation in CIDR-treated beef cattle", Domestic Animal Endocrinology, 33(1), pp.77~90 (2007), Elsevier BV[DOI]
- R. Manjarin, "Effect of prior FSH treatment on the estrus and ovulation responses to eCG in prepubertal gilts", Animal Reproduction Science, 110(1-2), pp.123~127 (2009), Elsevier BV[DOI]
- N.T. Kusina, "A comparison of the effects of progesterone sponges and ear implants, pgf2alpha, and their combination on efficacy of estrus synchronization and fertility of mashona goat does", Theriogenology, 53(8), pp.1567~1580 (2000), Elsevier BV[DOI]
- B. Leboeuf, "Efficacy of two types of vaginal sponges to control onset of oestrus, time of preovulatory LH peak and kidding rate in goats inseminated with variable numbers of spermatozoa", Theriogenology, 60(7), pp.1371~1378 (2003), Elsevier BV[DOI]
- H Twagiramungu, "Synchronization of ovarian follicular waves with a gonadotropin-releasing hormone agonist to increase the precision of estrus in cattle: a review", Journal of Animal Science, 73(10), pp.3141~3151 (1995), Oxford University Press (OUP)[DOI]
- R.L. Nebel, "Evaluation of Systematic Breeding Programs for Lactating Dairy Cows: A Review", Journal of Dairy Science, 81(4), pp.1169~1174 (1998), American Dairy Science Association[DOI]
- Hiroshi MIURA, "Relationships between the Conception Rate of Estrus Synchronization Using Estradiol Benzoate and CIDR (Progesterone) and Other Parameters in Holstein Lactating Dairy Cows", Journal of Reproduction and Development, 54(3), pp.214~216 (2008), Japanese Society of Animal Reproduction[DOI]
- Su T. LONG, "Effect of CIDR 12 to 19 days after AI on detection of returning estrus and conception rate in dairy cows", Journal of Reproduction and Development, 56(2), pp.251~255 (2010), Japanese Society of Animal Reproduction[DOI]
- C. Azevedo, "Comparison of fertility, regular returns-to-estrus, and calving interval between Ovsynch and CO-synch + CIDR protocols in dairy cows", Theriogenology, 82(6), pp.910~914 (2014), Elsevier BV[DOI]
- Calogero Stelletta, "Vulvar thermal pattern following synchronization of estrus is linked to fertility after timed artificial insemination in goat", Theriogenology, 103, pp.137~142 (2017), Elsevier BV[DOI]
- A.N.M.A. Rahman, "Estrus Synchronization and Superovulation in Goats: A Review", Journal of Biological Sciences, 8(7), pp.1129~1137 (2008), Science Alert[DOI]
- P. Quinn, "Effect of Purification of Bovine Serum Albumin on the Interaction of Human Semen with Mouse Ova in vitro", Biology of Reproduction, 22(2), pp.134~140 (1980), Oxford University Press (OUP)[DOI]
- T.S. Witte, "Effect of hen’s egg yolk on capacitation and acrosome reaction of diluted canine spermatozoa", Animal Reproduction Science, 110(3-4), pp.293~305 (2009), Elsevier BV[DOI]
- Jun Luo, "Research advances in reproduction for dairy goats", Animal Bioscience, 32(8), pp.1284~1295 (2019), 아세아·태평양축산학회[DOI]
- HH Titi, "Estrus Synchronization in Sheep and Goats Using Combinations of GnRH, Progestagen and Prostaglandin F2α", Reproduction in Domestic Animals, 45, pp.594~599 (2008), Wiley[DOI]
- J.S. Stevenson, "Treatment of Cycling and Noncycling Lactating Dairy Cows with Progesterone During Ovsynch", Journal of Dairy Science, 89(7), pp.2567~2578 (2006), American Dairy Science Association[DOI]
- A Menchaca, "Pregnancy Rate Obtained with Short-term Protocol for Timed Artificial Insemination in Goats", Reproduction in Domestic Animals, 42(6), pp.590~593 (2007), Wiley[DOI]
- J.R. Pursley, "Pregnancy Rates Per Artificial Insemination for Cows and Heifers Inseminated at a Synchronized Ovulation or Synchronized Estrus", Journal of Dairy Science, 80(2), pp.295~300 (1997), American Dairy Science Association[DOI]
- T W Geary, "Pregnancy rates of postpartum beef cows that were synchronized using Syncro-Mate-B or the Ovsynch protocol", Journal of Animal Science, 76(6), pp.1523~1527 (1998), Oxford University Press (OUP)[DOI]
- J.S. Stevenson, "Reproductive performance of dairy cows in various programmed breeding systems including Ovsynch and combinations of gonadotropin-releasing hormone and prostaglandin F2α", Journal of Dairy Science, 82(3), pp.506~515 (1999), American Dairy Science Association[DOI]
- Ainsworth L, "A controlled internal drug-release dispenser containing progesterone for control of the estrous cycle of ewes", Theriogenology, 26, pp.847~856 (1986)[DOI]
- Jonathan E. Wheaton, "CIDR: A new progesterone-releasing intravaginal device for induction of estrus and cycle control in sheep and goats", Animal Reproduction Science, 33(1-4), pp.127~141 (1993), Elsevier BV[DOI]
- M. Vilariño, "Re-use of intravaginal progesterone devices associated with the Short-term Protocol for timed artificial insemination in goats", Theriogenology, 75(7), pp.1195~1200 (2011), Elsevier BV[DOI]