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加味淸上補下湯의 물과 에탄올 추출법에 따른 抗알레르기 效果에 對한 硏究

Studying of the Effects of Gamichungsangboha-tang water-extract and ethanol-extract on IFN-γ, IL-4, T-bet, GATA-3, c-maf in Asthma Model

정희재|배현수|이형구|정승기|고은정|허태석
대한한방내과학회 (The Society Of Korean Oriental Internal Medicine)|2006.03|대한한방내과학회지 (The Journal of Korean Oriental Internal Medicine)|제27권 제1호|pp. 40-54

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Abstract

ABSTRACT Study of the Effects of Gamichungsangboha-tang Water Extract and Ethanol Extract on INF-γ, IL-4, T-bet, GATA-3 and c-maf in Asthma Model Objective : This study aimed to identify the different effects of GMCSBHT water-extract and ethanol-extract on Th1/Th2 differentiation by monitoring Th1/Th2 specific cytokine secretion patterns and the transcriptional activities of T-bet, GATA-3, c-maf, INF-γ and IL-4. Materials and Methods : Spleen cells from eight week-old BALB/c mice were cultured in GMCSBHT extracts containing medium without activation for 24 hours and with activation for 48 hours. CD4+ T cells were isolated and mRNA expression levels of INF-γ, IL-4, T-bet, GATA-3, c-maf by RT-PCR and secretion cytokines levels of INF-γ, IL-4 by ELISA were analyzed. Results : GMCSBHT extracts didn't have mitogenic effects on the unstimulated CD4+ T cells. In Th1 skewed condition, GMCSBAHT water extract had no significant effects on mRNA expression levels of INF-γ, T-bet and c-maf, but inhibited mRNA expression levels of IL-4, GATA-3. It showed significantly increased secretion cytokine levels of INF-γ, but had no significant effect on secretion cytokine levels of IL-4. In Th2 skewed condition, GMCSBHT ethanol extract inhibited mRNA expression levels of INF-γ, IL-4, GATA-3 and c-maf significantly, but had no significant effects on mRNA expression levels of T-bet. It had no significant effects on secretion cytokine levels of INF-γ, but showed remarkable inhibitory effects on secretion cytokine levels of IL-4. Conclusion : Results suggest that on Th1/Th2 deviation, GMCSBHT water extract has both amplifying effects on Th1 differentiation and inhibitory effects on Th2, but GMCSBHT ethanol extract has stronger inhibitory effects on Th2 differentiation than on Th1.

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관련 논문AI추천

加味淸上補下湯 열수 및 에탄올 추출물이 천식 관련 cytokine 분비에 미치는 영향김진주, 이건영, 이성헌, 이형구, 정승기, 정승연, 정희재, 황준호대한한방내과학회 · 대한한방내과학회지2006 · 제27권 제2호加味淸上補下湯이 Allergy성 기관지천식에 미치는 실험적 연구강필구, 김진일, 박동일, 우영식한의병리학회 · 동의생리병리학회지2002 · 제16권 제6호加味蘇子降氣湯이 천식 유발 병태 모델에서분자 및 조직병리학적 변화에 미치는 영향김동희, 안황용한의병리학회 · 동의생리병리학회지2006 · 제20권 제5호加味補中益氣湯이 알레르기반응에 미치는 영향에 대한 실험적 연구감철우, 김동혁, 박동일, 서수현, 신우진한의병리학회 · 동의생리병리학회지2002 · 제16권 제6호加味四君子湯이 OVA로 유발된 천식 마우스에 미치는 영향손지우, 신조영, 이시형대한한방내과학회 · 대한한방내과학회지2008 · 제29권 제2호加味貝母湯이 보조 T 임파구 분화에 미치는 영향고은정, 배현수, 신민규, 신재호, 정승기, 홍무창한의병리학회 · 동의생리병리학회지2004 · 제18권 제5호

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