導痰湯이 C2C12세포주로부터 myostatin발현에 의한 심근에 미치는 영향
Effect of Differentiation for Mouse Myoblast C2C12 Cells against Myostatin expression from Dodamtang
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Myostatin, a negative regulator of myogenesis, is shown to function by controlling the proliferation of myoblasts. In this study we show that myostatin is an inhibitor of myoblast differentiation and that this inhibition is mediated through Smad 3. To determine MyoD expression by Dodamtang treatment, we compared the expression pattern of C2C12 mouse myoblasts that constitutively express myostatin with control cells. In vitro, increasing concentrations of Dodamtang reversibly prevented the myogenic blockage of myoblasts by myostatin expression. ELISA assay, Western and confocal analysis indicated that treatment of Dodamtang to the low serum culture media increased the levels of MyoD leading to the inhibition of myogenic differentiation by myostatin. The stable transfection of C2C12 myoblasts with myostatin expressing constructs did rescue MyoD-induced myogenic differentiation. Consistent with this, the treatment of Dodamtang rescued the expression of a MyoD in C2C12 myoblasts treated with myostatin. Taken together, these results suggest that induction of MyoD by Dodamtang inhibits myostatin activity and expression via SMAD3 resulting in the rescue of the myoblasts to differentiate into myotubes. Thus we propose that myostatin action by Dodamtang plays a critical role in myogenic differentiation and that the muscular hyperplasia and hypertrophy seen in animals that blockage of functional myostatin is because of deregulated proliferation and differentiation of myoblasts.
Abstract
Myostatin, a negative regulator of myogenesis, is shown to function by controlling the proliferation of myoblasts. In this study we show that myostatin is an inhibitor of myoblast differentiation and that this inhibition is mediated through Smad 3. To determine MyoD expression by Dodamtang treatment, we compared the expression pattern of C2C12 mouse myoblasts that constitutively express myostatin with control cells. In vitro, increasing concentrations of Dodamtang reversibly prevented the myogenic blockage of myoblasts by myostatin expression. ELISA assay, Western and confocal analysis indicated that treatment of Dodamtang to the low serum culture media increased the levels of MyoD leading to the inhibition of myogenic differentiation by myostatin. The stable transfection of C2C12 myoblasts with myostatin expressing constructs did rescue MyoD-induced myogenic differentiation. Consistent with this, the treatment of Dodamtang rescued the expression of a MyoD in C2C12 myoblasts treated with myostatin. Taken together, these results suggest that induction of MyoD by Dodamtang inhibits myostatin activity and expression via SMAD3 resulting in the rescue of the myoblasts to differentiate into myotubes. Thus we propose that myostatin action by Dodamtang plays a critical role in myogenic differentiation and that the muscular hyperplasia and hypertrophy seen in animals that blockage of functional myostatin is because of deregulated proliferation and differentiation of myoblasts.
참고문헌 (40)
- 김철준, "일반보건학(2)", pp.226~238 (1996), 서울대학교출판부
- 屈松栢, "實用中醫心血病學" (2000), 科學技術文獻出版社
- 통계청, "2006년 사망원인 통계연보", pp.9~18 (2007), 통계청
- Tomida T, "Inhibition of COX-2 prevents hypertension and proteinuria associated with a decrease of 8-iso-PGF2alpha formation in L-NAME-treated rats", J Hypertens, 21(3), pp.601~609 (2003)
- Christou L, "Treatment of plasma cell leukemia with vincristine, liposomal oxorubicin and dexamethasone", Eur J Haematol, 67(1), pp.51~53 (2001)
- Long-Chun Xu, "Characterization of Myostatin Expressed with Semliki Forest Virus Replicon" (2005), Graduate school of Chungnam National University
- 유성란, "한우 myostatin 유전자의 SNP 및 발현분석", Jour. Agri. Sci, 31(2), pp.97~104 (2004)
- 嚴用和, "嚴氏濟生方", pp.79~ (1980), 人民衛生出版社
- 張介賓, "景岳全書", pp.79~ (1984), 上海科學技術出版社
- 李梴, "醫學入門", pp.525~ (1983), 한성사
- 許俊, "對譯東醫寶鑑", pp.277~ (1999), 법인문화사
- 이재희, "한방진료요방", pp.252~ (1983), 의학연구사
- 이상인, "천진처방해설", pp.443~444 (1987), 성보사
- 김태식, "당귀수산 및 導痰湯이 endotoxin으로 유발된 백서의 혈전증에 미치는 경향", 동의병리학회지, 3(1), pp.91~99 (1988)
- 김영균, "導痰湯이 고지혈증 실험동물에 미치는 효과" (1988), 원광대학교 대학원
- 김연두, "導痰湯이 家兎의 고지혈증 및 혈전증에 미치는 영향" (1992), 원광대학교 대학원
- 최정화, "導痰湯이 및 그 가미방이 국소뇌혈류량 및 혈압에 미치는 영향", 동의병리학회지, 14(1), pp.99~105 (2000)
- Naidu MU, "Protective effect of Gingko biloba extract against doxorubicin-induced cardiotoxicity in mice", Indian J Exp Biol, 40(8), pp.894~900 (2002)
- Ou J, "L-4F, an apolipoprotein A-1 mimetic, dramatically improves vasodilation in hypercholesterolemia and sickle cell disease", Circulation, 107(18), pp.2337~2341 (2003)
- Quiles JL, "Antioxidant nutrients and adriamycin toxicity", Toxicology, 180(1), pp.79~95 (2002)
- Godder K, "Heparanase activity in cultured endothelial cells", J Cell Physiol, 148(2), pp.274~280 (1999)
- Petrache I, "The role of the microtubules in tumor necrosis factor-alpha-induced endothelial cell permeability", Am J Respir Cell Mol Biol, 28(5), pp.574~581 (2003)
- Bresgen N, "Oxidative stress in cultured cerebral endothelial cells induces chromosomal aberrations, micronuclei, and apoptosis", J Neurosci Res, 72(3), pp.327~333 (2003)
- Wang QD, "Pharmacological possibilities for protection against myocardial reperfusion injury", Cardiovasc Res, 55(1), pp.25~37 (2003)
- Kang DH, "A role for uric acid in the progression of renal disease", J Am Soc Nephrol, 13(12), pp.2888~2897 (2002)
- 남기열, "人蔘의 품질과 약리활성 물질과의 상관성", J. Ginseng Res, 22(4), pp.274~ (1998)
- 유연희, "인삼알콜추출물이 혈관평활근의 수축력에 미치는 영향", 대한생리학회지, 13(1-2), pp.75~76 (1979)
- 한동훈, "失笑散 전탕액과 구성약물이 배양 심근세포의 LDH 활성도에 미치는 영향", 동의생리병리학회지, 15(5), pp.770~774 (2001), 대한동의병리학회
- 허윤, "生脈散이 대동맥 평활근 세포에서 NO 생성에 미치는 영향", 동의생리병리학회지, 15(5), pp.775~782 (2001), 대한동의병리학회
- 권강범, "沒藥 煎湯液이 培養 心筋細胞에 미치는 影響", 대한 한의학회지, 21(2), pp.79~86 (2000)
- 한동훈, "失笑散 전탕액이 XO", HX에 의해 손상된 배양 심근세포 박동수에 미치는 영향, 2001, pp.15(4), 566~570[DOI]
- 손창식, "丹蔘飮 전탕액이 산소유리기에 의해 손상된 심근세포의 LDH활성도에 미치는 영향", 동의생리병리학회지, 15(4), pp.621~625 (2001)
- 안효창, "括蔞薤白半夏湯 추출물이 배양 심근세포의 박동수와 LDH활성도에 미치는 영향", 동의생리병리학회지, 16(2), pp.289~295 (2002)
- 노현태, "加味淸心湯이 심혈관계 및 뇌손상에 미치는 영향", 한방성인병학회지, 4(1), pp.176~202 (1998)
- 전국한의과대학본초학교수, "본초학", pp.136~137 (1991), 영림사
- 신민교, "한약임상응용", pp.47~48 (1993), 전통의학연구소
- 박의수, "의증실험의결", pp.83~85 (1985), 성보사
- 김완희, "장부변증논치", pp.191~192 (1985), 성보사
- 정현우, "導痰湯이 백서의 뇌혈류개선 및 작용기전에 미치는 효과", 대한동의병리학회지, 14(2), pp.233~244 (2000)
- 조현경, "導痰湯이 뇌손상 및 고혈압에 미치는 영향", 대한한방내과학회지, 22(4), pp.503~512 (2001)