본문 바로가기
KERD
논문KCI 등재

Comparison of the Exopeptidase Activity of Fractions from Crude Extracts of Octopus Octopus vulgaris Cuvier Hepatopancreas Using Different Fractionation Methods

2

조회

0

다운로드

0

인용

0

참고문헌

키워드

주제분류수산학

초록

This study was performed to identify the optimum fractionation method and conditions to obtain exopeptidase-active fractions from octopus hepatopancreas (HP) crude extracts (CEs) using four techniques: solid ammonium sulfate fractionation, polyethylene glycol (PEG) fractionation, anion exchange chromatography, and gel filtration chromatography. The fractions with the highest total activity toward L-leucine-p-nitroanilide (Leu-pNA) were fraction IV from the ammonium sulfate and PEG fractionation, and fraction II in ion exchange and gel filtration chromatography. The total exoprotease activity of these fractions was highest in fraction IV (4,050.20 U) of ammonium sulfate fractionation, followed by fraction II (3,600.28 U) from gel filtration chromatography, fraction IV (2,861.30 U) from PEG fractionation, and fraction II (2,576.28 U) from ion exchange chromatography. These results suggest that ammonium sulfate fractionation using 60–80% ammonium sulfate was the most efficient method for separating the exoprotease active fractions from CEs of octopus HP.

Abstract

This study was performed to identify the optimum fractionation method and conditions to obtain exopeptidase-active fractions from octopus hepatopancreas (HP) crude extracts (CEs) using four techniques: solid ammonium sulfate fractionation, polyethylene glycol (PEG) fractionation, anion exchange chromatography, and gel filtration chromatography. The fractions with the highest total activity toward L-leucine-p-nitroanilide (Leu-pNA) were fraction IV from the ammonium sulfate and PEG fractionation, and fraction II in ion exchange and gel filtration chromatography. The total exoprotease activity of these fractions was highest in fraction IV (4,050.20 U) of ammonium sulfate fractionation, followed by fraction II (3,600.28 U) from gel filtration chromatography, fraction IV (2,861.30 U) from PEG fractionation, and fraction II (2,576.28 U) from ion exchange chromatography. These results suggest that ammonium sulfate fractionation using 60–80% ammonium sulfate was the most efficient method for separating the exoprotease active fractions from CEs of octopus HP.

참고문헌 (29)

  1. Results of the Fishery Production Survey in 2010, "Agriculture and Fishery Statistics Department", Statistics Korea, ~ (2010)
  2. Bougatef A, "Purification and characterization of trypsin from the viscera of sardine (Sardina pilchardus)", Food Chem, 102, pp.343~350 (2007)
  3. Bumberger E, "Bitter taste of enzymatic hydrolysates of casein. I. Isolation, structural and sensorial analysis of peptides from tryptic hydrolysates of beta-casein", Z Lebensm Unters Forsch, 197, pp.14~19 (1993)
  4. Chiou TK, "Purification and properties of an aminopeptidase from mullet, Mugil cephalus, roe", Agric Biol Chem, 52, pp.235~242 (1988)
  5. Clegg KM, "The structure of a bitter peptide derived from casein by digestion with papain", J Dairy Res, 41, pp.283~287 (1974)
  6. Dawson RMC, "Data for biochemical research. 3rd ed", pp.417~441 (1986), Oxford University Press
  7. Deejing S, "Purification and characterization of hyperthermotolerant leucine aminopeptidase from Geobacillus thermoleovorans 47b", J Ind Microbiol Biotechnol, 32, pp.269~276 (2005)
  8. Ezquerra-Brauer JM, "Influence of harvest season on the proteolytic activity of hepatopancreas and mantle tissues from jumbo squid (Doswicus gigas)", J Food Biochem, 26, pp.459~475 (2002)
  9. Garcia-Carreno FL, "Characterization of proteinase classes in langostilla (Pleuroncodes planipes) and crayfish (Pacifastacus astacus) extracts", J Food Biochem, 17, pp.97~113 (1993)
  10. Guerard F, "Maximising the Value of Marine By-products", pp.107~143 (2007), Woodhead Publishing Ltd
  11. Gupta R, "Bacterial alkaline proteases: molecular approaches and industrial applications", Appl Microbiol Biotechnolo, 59, pp.15~32 (2002)
  12. Haard NF, "Enzymes from food myosystems", J Muscle Foods, 1, pp.293~338 (1990)
  13. Hurtado JL, "Characterization of proteolytic activity in octopus (Octopus vulgaris) arm muscle", J Food Biochem, 23, pp.469~483 (1999)
  14. Izawa N, "Debittering of protein hydrolysates using Aeromonas caviae aminopeptidase", J Agric Food Chem, 45, pp.543~545 (1997)
  15. 김혜숙, "원양산 오징어(Illex argentinus) 내장의 endoprotease 및 exopeptidases의 분포 및 추출조건 검토", Applied Biological Chemistry, 50(4), pp.308~315 (2007), 한국응용생명화학회
  16. 김혜숙, "원양산 오징어(Illex argentinus) 내장으로부터 Endoprotease", 한국수산과학회지, 41(3), pp.176~181 (2008), 한국수산과학회
  17. 김혜숙, "원양산 오징어(Illex argentinus) 내장으로부터 Exopeptidase의 분획", 한국식품영양과학회지, 37(8), pp.1009~1017 (2008), 한국식품영양과학회
  18. Kishimura H, "Isolation and characteristics of trypsin inhibitor from the hepatopancreas of a squid (Todarodes pacificus)", Comp Biochem Physiol B Biochem Mol Biol, 130, pp.117~123 (2001)
  19. Kristinsson HG, "Fish protein hydrolysates: production, biochemical and functional properties", Crit Rev Food Sci Nutr, 40, pp.43~81 (2000)
  20. Lowry OH, "Protein measurement with the folin phenol reagent", J Biol Chem, 193, pp.265~275 (1951)
  21. Matoba T, "Isolation of bitter peptides from tryptic hydrolysate of casein and their chemical structure", Agric Biol Chem, 34, pp.1235~1243 (1970)
  22. Minagawa E, "Debittering mechanism in bitter peptides of enzymatic hydrolysates from milk casein by aminopeptidase T", J Food Sci, 54, pp.1225~1229 (1989)
  23. Raksakulthai R, "Purification and characterization of aminopeptidase fractions from squid (Illex illecebrosus) hepatopancreas", J Food Biochem, 23, pp.123~144 (1999)
  24. Raksakulthai R, "Accelerated cheddar cheese ripening with an aminopeptidase fraction from squid hepatopancreas", J Food Sci, 67, pp.923~929 (2002)
  25. Rao MB, "Molecular and biotechnological aspects of microbial proteases", Microbiol Mol Biol Rev, 62, pp.597~635 (1998)
  26. Sakai J, "Proteolytic enzymes of squid mantle muscle", Comp Biochem Physiol, 68, pp.389~395 (1981)
  27. Souissi N, "Preparation and use of media for protease-producing baterial strains based on by-products from cuttlefish (Sepia officinalis) and wastewaters from marine-products processing factories", Microbiol Res, 163, pp.473~480 (2008)
  28. Starky P. M, "Proteinases in Mammalian Cells and Tissues", pp.1977, Elsevier Science Ltd
  29. Umetsu H, "Mechanism of digestion of bitter peptides from soybean protein by wheat carboxypeptidase", J Jpn Soc Food Sci Technol, 35, pp.440~447 (1988)

관련 논문AI추천

Fractionation and Enzymatic Characterization of Endoprotease and Exopeptidase from Crude Extracts of Cuttlefish Sepia officinalis Hepatopancreas김기현, 김민지, 김진수, 김현정, 이정석, 허민수한국수산과학회 · Fisheries and Aquatic Sciences2012 · 제15권 제4호Endoprotease and Exopeptidase Activities in the Hepatopancreas of the Cuttlefish Sepia officinalis, the Squid Todarodes pacificus, and the Octopus Octopus vulgaris Cuvier김기현, 김민지, 김진수, 김현정, 허민수한국수산과학회 · Fisheries and Aquatic Sciences2012 · 제15권 제3호원양산 오징어(Illex argentinus) 간췌장 유래 Exopeptidase 분획물의 쓴맛개선 효과강상인, 김기현, 김민지, 김진수, 박성환, 이현지, 허민수한국수산과학회 · 한국수산과학회지2014 · 제47권 제2호원양산 오징어(Illex argentinus) 내장으로부터 Endoprotease김진수, 김혜숙, 허민수한국수산과학회 · 한국수산과학회지2008 · 제41권 제3호살 오징어(Todarodes pacificus) 간췌장 유래 한외여과 Aminopeptidase Retentate Fraction의 특성과 쓴맛 개선효과강상인, 김진수, 박선영, 윤인성, 이정석, 정우철, 허민수한국수산과학회 · 한국수산과학회지2020 · 제53권 제1호살 오징어(Todarodes pacificus) 간췌장 유래 Aminopeptidase 활성획 분에 의한 Alcalase 처리 멸치(Engrauris japonica) 가수분해물의 쓴맛 개선 최적화권인상, 김진수, 윤인성, 이정석, 허민수한국수산과학회 · 한국수산과학회지2021 · 제54권 제5호

이 간행물의 다른 논문 (14건)