본문 바로가기
KERD
논문KCI 등재

Chitosan-Coated Fe3O4 Magnetic Nanoparticles as Carrier of Cisplatin for Drug Delivery

2

조회

0

다운로드

1

인용

0

참고문헌

키워드

주제분류수산학

초록

A synthesis method for a chitosan-coated magnetic drug-delivery system of cisplatin is proposed. Here, cisplatin was conjugated to the surface of Magnetite (Fe3O4) nanoparticles via a (3-Aminopropyl)-trimethoxysilane (APTS) coupling agent. To reduce the cytotoxic effect of cisplatin, the magnetic drug was then encapsulated in chitosan (CS-cisplatin-Fe3O4) through the water/oil (W/O) emulsion method. The CS-cisplatin-Fe3O4 nanoparticles were synthesized in a spherical shape with a diameter of 190 nm. The cytotoxicity assay was performed using HeLa cells. The cisplatin uptake of the cells was determined using High Performance Liquid Chromatography (HPLC) to calculate the drug content. The controlled release of cisplatin was demonstrated by regulating the dissolution and diffusion of the drug through the chitosan matrix.

Abstract

A synthesis method for a chitosan-coated magnetic drug-delivery system of cisplatin is proposed. Here, cisplatin was conjugated to the surface of Magnetite (Fe3O4) nanoparticles via a (3-Aminopropyl)-trimethoxysilane (APTS) coupling agent. To reduce the cytotoxic effect of cisplatin, the magnetic drug was then encapsulated in chitosan (CS-cisplatin-Fe3O4) through the water/oil (W/O) emulsion method. The CS-cisplatin-Fe3O4 nanoparticles were synthesized in a spherical shape with a diameter of 190 nm. The cytotoxicity assay was performed using HeLa cells. The cisplatin uptake of the cells was determined using High Performance Liquid Chromatography (HPLC) to calculate the drug content. The controlled release of cisplatin was demonstrated by regulating the dissolution and diffusion of the drug through the chitosan matrix.

참고문헌 (34)

  1. Arum Y, "Controlling the optimum dose of AMPTS functionalized-magnetite nanoparticles for hyperthermia cancer therapy", Appl Nanosci, 1, pp.237~246 (2011)
  2. Bae KH, "Chitosan oligosaccharide-stabilized ferrimagnetic iron oxide nanocubes for magnetically modulated cancer hyperthermia", ACS nano, 6, pp.5266~5273 (2012)
  3. Bhattarai N, "Chitosan-based hydrogels for controlled, localized drug delivery", Adv Drug Deliv Rev, 62, pp.83~99 (2010)
  4. Bhowmick T, "In vitro effects of cisplatin-functionalized silica nanoparticles on chondrocytes", J Nanopart Res, 12, pp.2757~2770 (2010)
  5. De Palma R, "Silane ligand exchange to make hydrophobic superparamagnetic nanoparticles water-dispersible", Chem Mater, 19, pp.1821~1831 (2007)
  6. Gonzalez VM, "Is cisplatininduced cell death always produced by apoptosis? Molec Pharmacol 59, 657-663", Molec Pharmacol, 59 (2001)
  7. Ishihara M, "Chitosan hydrogel as a drug delivery carrier to control angiogenesis", J Artific Org : Offic J Japanese Soc Artific Org, 9, pp.8~16 (2006)
  8. Ishiyama M, "A combined assay of cell viability and in vitro cytotoxicity with a highly water-soluble tetrazolium salt, neutral red and crystal violet", Biol Pharmac Bull, 19, pp.1518~1520 (1996)
  9. Ito A, "Magnetite nanoparticle-loaded anti-HER2 immunoliposomes for combination of antibody therapy with hyperthermia", Cancer lett, 212, pp.167~175 (2004)
  10. Jamieson ER, "Structure, recognition, and processing of cisplatin-DNA adducts", Chem Rev, 99, pp.2467~2498 (1999)
  11. Jana NR, "Synthesis of water-soluble and functionalized nanoparticles by silica coating", Chem Mater, 19, pp.5074~5082 (2007)
  12. Jayakumar R, "Biomaterials based on chitin and chitosan in wound dressing applications", Biotechnol Advanc, 29, pp.322~337 (2011)
  13. Jena P, "Toxicity and antibacterial assessment of chitosan-coated silver nanoparticles on human pathogens and macrophage cells", Intern J Nanomedic, 7, pp.1805~1818 (2012)
  14. Kim DK, "Starch-coated superparamagnetic nanoparticles as MR contrast agents", Chem Mater, 15, pp.4343~4351 (2003)
  15. Kohler N, "Methotrexate-modified superparamagnetic nanoparticles and their intracellular uptake into human cancer cells", Langmuir : ACS J Surfac Coll, 21, pp.8858~8864 (2005)
  16. Kohli E, "Formulations of biodegradable Nanogel carriers with 5’-triphosphates of nucleoside analogs that display a reduced cytotoxicity and enhanced drug activity", J Contr Rel : Offic J Contr Rel Soc, 121, pp.19~27 (2007)
  17. Komeda S, "The phosphate clamp : a small and independent motif for nucleic acid backbone recognition", Nucl Acid Res, 39, pp.325~336 (2011)
  18. Kurita K, "Chitin and chitosan : functional biopolymers from marine crustaceans", Mar Biotechnol, 8, pp.203~226 (2006)
  19. Laurienzo P, "Marine polysaccharides in pharmaceutical applications : an overview", Mar Drug, 8, pp.2435~2465 (2010)
  20. Lopez-Flores A, "A high-performance liquid chromatographic assay for determination of cisplatin in plasma, cancer cell, and tumor samples", J Pharmacol Toxicol Meth, 52, pp.366~372 (2005)
  21. Mi FL, "Fabrication and characterization of a sponge-like asymmetric chitosan membrane as a wound dressing", Biomaterials, 22, pp.165~173 (2001)
  22. Oh J, "Detection of magnetic nanoparticles in tissue using magneto-motive ultrasound", Nanotechnology, 17, pp.4183~4190 (2006)
  23. Oh J, "Magneto-motive detection of tissue-based macrophages by differential phase optical coherence tomography", Laser Surger Medic, 39, pp.266~272 (2007)
  24. Onishi H, "Biodegradation and distribution of water-soluble chitosan in mice", Biomaterials, 20, pp.175~182 (1999)
  25. Park J, "Ultra-large-scale syntheses of monodisperse nanocrystals", Nat Mater, 3, pp.891~895 (2004)
  26. Reedijk J, "Why does Cisplatin reach Guanine-n7 with competing s-donor ligands available in the cell?", Chem Rev, 99, pp.2499~2510 (1999)
  27. Sogias IA, "Why is chitosan mucoadhesive?", Biomacromolecules, 9, pp.1837~1842 (2008)
  28. Suh JK, "Application of chitosan-based polysaccharide biomaterials in cartilage tissue engineering : a review", Biomaterials, 21, pp.2589~2598 (2000)
  29. Sun X, "Size-Controlled Synthesis of Magnetite(Fe3O4)Nanoparticles coated with glucose and gluconic acid from a single Fe(III)precursor by a sucrose bifunctional hydrothermal method", J Physic Chem C, 113, pp.16002~16008 (2009)
  30. Thanou M, "Oral drug absorption enhancement by chitosan and its derivatives", Advanc Drug Deliv Rev, 52, pp.117~126 (2001)
  31. Wang YM, "Optimization of the formulation design of chitosan microspheres containing cisplatin", J Pharmac Sci, 85, pp.1204~1210 (1996)
  32. Wu P, "Silanation of nanostructured mesoporous magnetic particles for heavy metal recovery", Indus Engineer Chem Res, 44, pp.816~824 (2005)
  33. Zhang JQ, "Lyophilized paclitaxel magnetoliposomes as a potential drug delivery system for breast carcinoma via parenteral administration: in vitro and in vivo studies", Pharmac Res, 22, pp.573~583 (2005)
  34. Zhu L, "Chitosan-coated magnetic nanoparticles as carriers of 5-fluorouracil: preparation, characterization and cytotoxicity studies", Coll Surf B, Biointerfaces, 68, pp.1~6 (2009)

관련 논문AI추천

이 간행물의 다른 논문 (14건)