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消平湯이 RIN-m5F 세포에서 인슐린 분비 및 유전자 발현에 미치는 영향

Effect of Sopyung-tang Extract on Insulin Secretion and Gene Expression in RIN-m5F Cells

조충식|윤성식
대한한방내과학회 (The Society Of Korean Oriental Internal Medicine)|2010.03|대한한방내과학회지 (The Journal of Korean Oriental Internal Medicine)|제31권 제1호|pp. 25-39

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참고문헌

키워드

Gene expressionGlucotoxicityInsulin secretionSopyung-tang(SPT)RIN-m5F
주제분류한의학

초록

Background : At high glucose levels in β-cells, cell viability and insulin secretion are decreased by glucotoxicity. Sopyung -tang(SPT) had an effect on blood glucose level decrease and antioxidant enzyme activities in streptozotocin-induced diabetic rats. Objectives : This study performed a series of experiment to verify the effects of SPT extract on the cell viability, antioxidant enzyme activities, insulin secretion and insulin mRNA expression at hyperglycemic states of RIN-m5F. Methods : After treatment at various concentrations of SPT added to the RIN-m5F cells, cell viability by MTT assay, free radical-scavenging activity, SOD activity and insulin secretion were measured. Additionally, insulin-related gene expression was measured using real-time RT-PCR. Results : Compared to the control group, SPT extract showed considerable effects on RIN-m5F cell viability, DPPH radical-scavenging activity, superoxide dismutase (SOD) activity, insulin secretion and insulin-related gene expression. Conclusions : This study showed that SPT extract has an effect on β-cell cell viability, insulin secretion and insulin -related gene expression. Thus, SPT extract may be used for treatment of diabetes and its complications. Further mechanism studies of SPT seem to be necessary on the glucotoxicity and oxidative stress.

Abstract

Background : At high glucose levels in β-cells, cell viability and insulin secretion are decreased by glucotoxicity. Sopyung -tang(SPT) had an effect on blood glucose level decrease and antioxidant enzyme activities in streptozotocin-induced diabetic rats. Objectives : This study performed a series of experiment to verify the effects of SPT extract on the cell viability, antioxidant enzyme activities, insulin secretion and insulin mRNA expression at hyperglycemic states of RIN-m5F. Methods : After treatment at various concentrations of SPT added to the RIN-m5F cells, cell viability by MTT assay, free radical-scavenging activity, SOD activity and insulin secretion were measured. Additionally, insulin-related gene expression was measured using real-time RT-PCR. Results : Compared to the control group, SPT extract showed considerable effects on RIN-m5F cell viability, DPPH radical-scavenging activity, superoxide dismutase (SOD) activity, insulin secretion and insulin-related gene expression. Conclusions : This study showed that SPT extract has an effect on β-cell cell viability, insulin secretion and insulin -related gene expression. Thus, SPT extract may be used for treatment of diabetes and its complications. Further mechanism studies of SPT seem to be necessary on the glucotoxicity and oxidative stress.

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