Gut microbiota profiling in aged dogs after feeding pet food contained Hericium erinaceus
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Health concern of dogs is the most important issue for pet owners. People who have companiedthe dogs long-term provide the utmost cares for their well-being and healthy life. Recently,it was revealed that the population and types of gut microbiota affect the metabolismand immunity of the host. However, there is little information on the gut microbiome of dogs. Hericium erinaceus (H. erinaceus; HE) is one of the well-known medicinal mushrooms andhas multiple bioactive components including polyphenol, β-glucan, polysaccharides, ergothioneine,hericerin, erinacines, etc. Here we tested a pet food that contained H. erinaceusfor improvement in the gut microbiota environment of aged dogs. A total of 18 dogs, each 11years old, were utilized. For sixteen weeks, the dogs were fed with 0.4 g of H. erinaceus (HEL),or 0.8 g (HE-H), or without H. erinaceus (CON) per body weight (kg) with daily diets (n = 6per group). Taxonomic analysis was performed using metagenomics to investigate the differencein the gut microbiome. Resulting from principal coordinates analysis (PCoA) to confirmthe distance difference between the groups, there was a significant difference between HE-Hand CON due to weighted Unique fraction metric (Unifrac) distance (p = 0.047), but HE-L didnot have a statistical difference compared to that of CON. Additionally, the result of Lineardiscriminate analysis of effect size (LEfSe) showed that phylum Bacteroidetes in HE-H andits order Bacteroidales increased, compared to that of CON, Additionally, phylum Firmicutesin HE-H, and its genera (Streptococcus, Tyzzerella) were reduced. Furthermore, at the familylevel, Campylobacteraceae and its genus Campylobacter in HE-H was decreased comparedto that of CON. Summarily, our data demonstrated that the intake of H. erinaceus can regulatethe gut microbial community in aged dogs, and an adequate supply of HE on pet dietswould possibly improve immunity and anti-obesity on gut-microbiota in dogs.
Abstract
Health concern of dogs is the most important issue for pet owners. People who have companiedthe dogs long-term provide the utmost cares for their well-being and healthy life. Recently,it was revealed that the population and types of gut microbiota affect the metabolismand immunity of the host. However, there is little information on the gut microbiome of dogs. Hericium erinaceus (H. erinaceus; HE) is one of the well-known medicinal mushrooms andhas multiple bioactive components including polyphenol, β-glucan, polysaccharides, ergothioneine,hericerin, erinacines, etc. Here we tested a pet food that contained H. erinaceusfor improvement in the gut microbiota environment of aged dogs. A total of 18 dogs, each 11years old, were utilized. For sixteen weeks, the dogs were fed with 0.4 g of H. erinaceus (HEL),or 0.8 g (HE-H), or without H. erinaceus (CON) per body weight (kg) with daily diets (n = 6per group). Taxonomic analysis was performed using metagenomics to investigate the differencein the gut microbiome. Resulting from principal coordinates analysis (PCoA) to confirmthe distance difference between the groups, there was a significant difference between HE-Hand CON due to weighted Unique fraction metric (Unifrac) distance (p = 0.047), but HE-L didnot have a statistical difference compared to that of CON. Additionally, the result of Lineardiscriminate analysis of effect size (LEfSe) showed that phylum Bacteroidetes in HE-H andits order Bacteroidales increased, compared to that of CON, Additionally, phylum Firmicutesin HE-H, and its genera (Streptococcus, Tyzzerella) were reduced. Furthermore, at the familylevel, Campylobacteraceae and its genus Campylobacter in HE-H was decreased comparedto that of CON. Summarily, our data demonstrated that the intake of H. erinaceus can regulatethe gut microbial community in aged dogs, and an adequate supply of HE on pet dietswould possibly improve immunity and anti-obesity on gut-microbiota in dogs.
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