Methods to eradicate soft tunic syndrome (STS)-causing protozoa Azumiobodo hoyamushi, the highly infectious parasite from the edible ascidian (Halocynthia roretzi)
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Although soft tunic syndrome (STS) in the ascidian is a serious disease, helpful measures have yet not been established. It was examined in this study by applying aniti-parasitic drugs to eradicate the causative protozoa Azumiobodo hoyamushi from infected ascidians. Formalin was synergistic in killing parasites in vitro when co-treated with hydrogen peroxide (H2O2) or bronopol, but not with chloramine-T or povidone-iodine (PVP-I), when tested with in vitro parasite culture. The synergistic effects did not change when formalin-H2O2 (or bronopol) ratios were changed. It was found that treatment periods less than 60 min achieved a sub-maximal efficacy. Increasing drug concentration while keeping 30 min period improved anti-parasitic effects. Anti-parasitic effects of formalin(F) + H2O2(H) were also assessed in an in vivo STS model infected with cultured parasites. It was observed that combined 50 (40F + 10H) and 100 (80F +20H) ppm were effective in partially preventing STS-caused mortality. In horizontally transmitted artificial STS model, significant prevention of ascidian mortality was also observed after 50 ppm. Marked reduction of living parasites were noted after drug treatments in vivo. The results provide a highly useful basis to develop a preventive or treatment measure against the currently uncontrollable STS in the ascidian.
Abstract
Although soft tunic syndrome (STS) in the ascidian is a serious disease, helpful measures have yet not been established. It was examined in this study by applying aniti-parasitic drugs to eradicate the causative protozoa Azumiobodo hoyamushi from infected ascidians. Formalin was synergistic in killing parasites in vitro when co-treated with hydrogen peroxide (H2O2) or bronopol, but not with chloramine-T or povidone-iodine (PVP-I), when tested with in vitro parasite culture. The synergistic effects did not change when formalin-H2O2 (or bronopol) ratios were changed. It was found that treatment periods less than 60 min achieved a sub-maximal efficacy. Increasing drug concentration while keeping 30 min period improved anti-parasitic effects. Anti-parasitic effects of formalin(F) + H2O2(H) were also assessed in an in vivo STS model infected with cultured parasites. It was observed that combined 50 (40F + 10H) and 100 (80F +20H) ppm were effective in partially preventing STS-caused mortality. In horizontally transmitted artificial STS model, significant prevention of ascidian mortality was also observed after 50 ppm. Marked reduction of living parasites were noted after drug treatments in vivo. The results provide a highly useful basis to develop a preventive or treatment measure against the currently uncontrollable STS in the ascidian.
참고문헌 (23)
- Bell A, "Antimalarial drug synergism and antagonism : mechanism and clinical significance", FEMS Microbiol Lett, 253, pp.171~184 (2005)
- Bower SM, "Disinfectants and therapeutic agents for controlling Labyrinthuloides haliotidis(Protozoa : Labyrinthomorpha), an abalone pathogen", Aquculture, 78, pp.207~215 (1989)
- Cho HK, "Identification of softness syndrome-associated candidate genes and DNA sequence variation in the sea squirt, Halocynthia roretzi", Mar Biotechnol, 10, pp.447~456 (2008)
- Gilbert JP, "An in vitro method for testing synergistic action of parasiticides using malachite green and formalin as a model system", J Fish Dis, 2, pp.191~196 (1979)
- Harms CA, "Treatments for parasitic diseases of aquarium and ornamental fish", Sem Avian Exot Pet Med, 5, pp.54~63 (1996)
- 지보영, "양식 어류와 이들 난에 대한 항곰팡이성 약물들의 효과 비교", 한국수산과학회지, 42(1), pp.34~40 (2009), 한국수산과학회
- Kim HJ, "The kinetoplastid parasite Azumiobodo hoyamushi, the causative agent of soft tunic syndrome of the sea squirt Halocynthia roretzi, resides in the East Sea of Korea", J Invert Pathol, 116, pp.36~42 (2014)
- Kitamura SI, "Tunicate morphology and viral surveillance in diseased Korean ascidians : soft tunic syndrome in the edible ascidians, Halocynthia roretzi(Drasche), in aquaculture", J Fish Dis, 33, pp.153~160 (2010)
- Klaasen CD, "The pharmacological basis of therapeutics", pp.67~80 (2001), McGraw-Hill
- Kumagai A, "Mass mortality of cultured ascidians Halocynthia roretzi associated with softening of the tunic and flagellate-like cells", Dis Aquat Org, 90, pp.223~234 (2010)
- Kumagai A, "Soft tunic syndrome in the edible ascidian Halocynthia roretzi is caused by a kinetoplastid protist", Dis Aquat Org, 95, pp.153~161 (2011)
- 이재근, "멍게, Halocynthia roretzi 물렁증의 원인충인 Azumiobodo hoyamushi의 살충효과 평가를 위한 현미경계수법과 alamar blue assay 비교", 한국어병학회지, 26(1), pp.31~38 (2013), 한국어병학회
- Maris P, "Modes of action of disinfectants", Rev Sic Tech Off Int Epiz, 14, pp.47~55 (1995)
- Mason JT, "Effects of formaldehyde fixation on protein secondary structure : a calorimetric and infrared spectroscopic investigation", J Histochem Cytochem, 39, pp.225~329 (1991)
- McDonnell G, "Antiseptic and disinfectants : activity, actions, and resistance", Clin Microbiol Rev, 12, pp.147~179 (1999)
- Mutschuler E, "Drug actions", pp.799~ (1995), CRC Press
- Park KH, "In vitro and in vivo efficacy of drugs against the protozoan parasite Azumiobodo hoyamushi that causes soft tunic syndrome in the edible ascidian Halocynthia roretzi(Drasche)", J Fish Dis, 37, pp.309~317 (2014)
- Russo R, "Preliminary investigations of hydrogen peroxide treatment of selected ornamental fishes and efficacy against external bacteria and parasites in green swordtails", J Aquat Anim Health, 19, pp.121~127 (2007)
- Shinn AP, "The anti-protozoal activity of bronopol on the key life-stages of Ichthyophthirius multifiliis Fouquet, 1986(Ciliophora)", Vet Parasitol, 186, pp.229~236 (2012)
- Shinn A, "The anti-protozoal activity of bronopol on the key life-stages of Ichthyophthrius multifiliis Fouquet, 1876(Ciliophora)", Vet Parasitology, 186, pp.229~236 (2012)
- Smith P., "Infectious disease in aquaculture: prevention and control", pp.161~189 (2012), Woodhead Publishing
- Stretton RJ, "Some aspects of the mode of action of the antibacterial compound bronopol (2-bromo-2-nitropropan-1,3-diol)", J Appl Bacteriol, 36, pp.61~76 (1973)
- Treves-Brown KM, "Applied fish pharmacology", pp.161~179 (2000), Kluwer Academic Publishers