Establishment and long-term culture of the cell lines derived from gonad tissues of Siberian sturgeon (Acipenser baerii)
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
To culture germline stem cells in vitro, establishment of the cell lines that can be used as the feeder cells is a prerequisite. In this study, we tried to establish gonad-derived cell lines in Siberian sturgeon (Acipenser baerii). Five 1-year-old A. baerii were used as a donor of gonad tissues, and gonad-dissociated cells were cultured in vitro. Subsequently, determination of growth conditions, long-term culture, characterization, and cryopreservation of the cell lines were also conducted. Five gonad-derived cell lines were stably established and cultured continuously over at least the 73th passage and 402 culture days under the media containing 20 % fetal bovine serum at 28 °C. All cell lines consisted of two main cell types based on morphology even if the ratio of the two cell types was different depending on cell lines. Despite long-term culture, all cell lines maintained diploid DNA contents and expression of several genes that are known to express in the A. baerii gonad. After freezing and thawing of the cell lines, post-thaw cell viabilities between 57.6 and 92.9 % depending on cell lines were indentified, suggesting that stable cryopreservation is possible. The results and the cell lines established in this study will contribute to the development of an in vitro system for A. baerii germline stem cell culture.
Abstract
To culture germline stem cells in vitro, establishment of the cell lines that can be used as the feeder cells is a prerequisite. In this study, we tried to establish gonad-derived cell lines in Siberian sturgeon (Acipenser baerii). Five 1-year-old A. baerii were used as a donor of gonad tissues, and gonad-dissociated cells were cultured in vitro. Subsequently, determination of growth conditions, long-term culture, characterization, and cryopreservation of the cell lines were also conducted. Five gonad-derived cell lines were stably established and cultured continuously over at least the 73th passage and 402 culture days under the media containing 20 % fetal bovine serum at 28 °C. All cell lines consisted of two main cell types based on morphology even if the ratio of the two cell types was different depending on cell lines. Despite long-term culture, all cell lines maintained diploid DNA contents and expression of several genes that are known to express in the A. baerii gonad. After freezing and thawing of the cell lines, post-thaw cell viabilities between 57.6 and 92.9 % depending on cell lines were indentified, suggesting that stable cryopreservation is possible. The results and the cell lines established in this study will contribute to the development of an in vitro system for A. baerii germline stem cell culture.
참고문헌 (43)
- Amit M, "Human feeder layers for human embryonic stem cells", Biol Reprod, 68, pp.2150~2156 (2003)
- Aponte PM, "Propagation of bovine spermatogonial stem cells in vitro", Reproduction, 136, pp.543~557 (2008)
- Berbejillo J, "Expression and phylogeny of candidate genes for sex differentiation in a primitive fish species, the Siberian sturgeon, Acipenser baerii", Mol Reprod Dev, 79, pp.504~516 (2012)
- Brinster RL, "Male germline stem cells : from mice to men", Science, 316, pp.404~405 (2007)
- Evans MJ, "Establishment in culture of pluripotential cells from mouse embryos", Nature, 292, pp.154~156 (1981)
- Fan L, "Zebrafish primordial germ cell cultures derived from vasa : : Rfp transgenic embryos", Stem Cells Dev, 17, pp.585~597 (2008)
- Freshney RI., "Culture of animal cells: a manual of basic technique and specialized applications" (2010), Wiley-Blackwell
- Gisbert E, "Ontogenetic behavior of Siberian sturgeon, Acipenser baerii: a synthesis between laboratory tests and field data", Environ Biol Fish, 67, pp.311~319 (2003)
- Guan K, "Pluripotency of spermatogonial stem cells from adult mouse testis", Nature, 440, pp.1199~1203 (2006)
- Hofmann MC, "Establishment and characterization of neonatal mouse Sertoli cell lines", J Androl, 24, pp.120~130 (2003)
- Hong Y, "Establishment of a normal medakafish spermatogonial cell line capable of sperm production in vitro", Proc Natl Acad Sci U S A, 101, pp.8011~8016 (2004)
- Hong N, "Fish stem cell cultures", Int J Biol Sci, 7, pp.392~402 (2011)
- Jing D, "Hematopoietic stem cells in co-culture with mesenchymal stromal cells—modeling the niche compartments in vitro", Haematologica, 95, pp.542~550 (2010)
- Kanatsu-Shinohara M, "Long-term proliferation in culture and germline transmission of mouse male germline stem cells", Biol Reprod, 69, pp.612~616 (2003)
- Kanatsu-Shinohara M, "Reconstitution of mouse spermatogonial stem cell niches in culture", Cell Stem Cell, 11, pp.567~578 (2012)
- Kawasaki T, "Production of zebrafish offspring from cultured spermatogonial stem cells", Genes to Cells, 17, pp.316~325 (2012)
- Keyvanshokooh S, "A review of sex determination and searches for sex-specific markers in sturgeon", Aquac Res, 41, pp.e1~e7 (2010)
- Kim MS, "Establishment condition and characterization of heart-derived cell culture in Siberian sturgeon(Acipenser baerii)", In Vitro Cell Dev Biol Anim, 50, pp.909~917 (2014)
- Kubota H, "Growth factors essential for self-renewal and expansion of mouse spermatogonial stem cells", Proc Natl Acad Sci U S A, 101, pp.16489~16494 (2004)
- Lacerda SM, "Phenotypic characterization and in vitro propagation and transplantation of the Nile tilapia(Oreochromis niloticus)spermatogonial stem cells", Gen Comp Endocrinol, 192, pp.95~106 (2013)
- Lacerda SM, "Biology and identity of fish spermatogonial stem cell", Gen Comp Endocrinol, 207, pp.56~65 (2014)
- Lakra WS, "Development, characterization, conservation and storage of fish cell lines : a review", Fish Physiol Biochem, 37, pp.1~20 (2011)
- Lee WY, "Establishment and in vitro culture of porcine spermatogonial germ cells in low temperature culture conditions", Stem Cell Res, 11, pp.1234~1249 (2013)
- Lee KH, "Identification and in vitro derivation of spermatogonia in beagle testis", PLoS ONE, 9, pp.e109963~ (2014)
- Lehmann R, "Germline stem cells : origin and destiny", Cell Stem Cell, 10, pp.729~739 (2012)
- Li Z, "Formation and cultivation of medaka primordial germ cells", Cell Tissue Res, 357, pp.71~81 (2014)
- Loir M, "Trout Sertoli cells and germ cells in primary culture : morphological and ultrastructural study", Gamete Res, 24, pp.151~169 (1989)
- Matsui Y, "Derivation of pluripotential embryonic stem cells from murine primordial germ cells in culture", Cell, 70, pp.841~847 (1992)
- Nagano M, "Maintenance of mouse male germ line stem cells in vitro", Biol Reprod, 68, pp.2207~2214 (2003)
- Pacchiarotti J, "Differentiation potential of germ line stem cells derived from the postnatal mouse ovary", Differentiation, 79, pp.159~170 (2010)
- Park TS, "Production of quail(Coturnix japonica)germline chimeras derived from in vitro-cultured gonadal primordial germ cells", Mol Reprod Dev, 75, pp.274~281 (2008)
- Ruban G, "Acipenser baerii", The IUCN Red List of Threatened Species 2010: e.T244A13046607, ~ (2010)
- Sakai N, "Transmeiotic differentiation of zebrafish germ cells into functional sperm in culture", Development, 129, pp.3359~3365 (2002)
- Shikina S, "Improved in vitro culture conditions to enhance the survival, mitotic activity, and transplantability of rainbow trout type a spermatogonia", Biol Reprod, 83, pp.268~276 (2010)
- Shikina S, "Culture conditions for maintaining the survival and mitotic activity of rainbow trout transplantable type A spermatogonia", Mol Reprod Dev, 75, pp.529~537 (2008)
- Song Y, "Characteristics of long-term cultures of avian primordial germ cells and gonocytes", Biol Reprod, 90, pp.15~ (2014)
- Spradling A, "Germline stem cells", Cold Spring Harb Perspect Biol, 3, pp.a002642~ (2011)
- Takahashi K, "Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors", Cell, 126, pp.663~676 (2006)
- Tiptanavattana N, "Characterization and in vitro culture of putative spermatogonial stem cells derived from feline testicular tissue", J Reprod Dev, 59, pp.189~195 (2013)
- Wong TT, "Dorsomorphin promotes survival and germline competence of zebrafish spermatogonial stem cells in culture", PLoS ONE, 8, pp.e71332~ (2013)
- Wong TT, "Production of zebrafish offspring from cultured female germline stem cells", PLoS ONE, 8, pp.e62660~ (2013)
- Zhang Y, "Production of transgenic mice by random recombination of targeted genes in female germline stem cells", J Mol Cell Biol, 3, pp.132~141 (2011)
- Zhou GZ, "Establishment of a Chinese sturgeon Acipenser sinensis tail-fin cell line and its susceptibility to frog iridovirus", J Fish Biol, 73, pp.2058~2067 (2008)