잉어(Cyprinus carpio )에서 어류 구충제에 대한 N -acetylcysteine(NAC)의 독성 저감 효과
Attenuating Effects of N-acetylcysteine (NAC) against Fish Parasiticide-induced Toxicity in Carp Cyprinus carpio
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초록
This study examined whether N-acetylcysteine (NAC), a glutathione precursor, could attenuate toxic effects of three fish anti-parasitic agents, trichlorfon, hydrogen peroxide (H2O2) and formalin, all of which are known to exert side effects through free radical production. Common carp Cyprinus carpio were fed with NAC (approx. 50 mg/kg/day) for 3 consecutive days prior to anti-parasite bathing for a 24 hr period. Mortality rates were examined during this 24 hr bathing period, and selected hematological and biochemical parameters were also assessed at the termination of anti-parasite exposure. The mortality rates and plasma glucose elevations caused by all three anti-parasitics were significantly reduced by NAC pretreatment. Trichlorfon, but not H2O2 or formalin, elevated plasma levels of aspartatetransaminase (AST) and alanine-transaminase (ALT), and these elevations were attenuated by NAC. There was no change in hematocrit values in any treatment. The results provide evidence for the attenuating effects of NAC against toxicity caused by anti-parasite agents that act through free radical-producing properties. The results found in this study also suggest that NAC may be administered to fish to minimize toxicity in fish parasiticide use.
Abstract
This study examined whether N-acetylcysteine (NAC), a glutathione precursor, could attenuate toxic effects of three fish anti-parasitic agents, trichlorfon, hydrogen peroxide (H2O2) and formalin, all of which are known to exert side effects through free radical production. Common carp Cyprinus carpio were fed with NAC (approx. 50 mg/kg/day) for 3 consecutive days prior to anti-parasite bathing for a 24 hr period. Mortality rates were examined during this 24 hr bathing period, and selected hematological and biochemical parameters were also assessed at the termination of anti-parasite exposure. The mortality rates and plasma glucose elevations caused by all three anti-parasitics were significantly reduced by NAC pretreatment. Trichlorfon, but not H2O2 or formalin, elevated plasma levels of aspartatetransaminase (AST) and alanine-transaminase (ALT), and these elevations were attenuated by NAC. There was no change in hematocrit values in any treatment. The results provide evidence for the attenuating effects of NAC against toxicity caused by anti-parasite agents that act through free radical-producing properties. The results found in this study also suggest that NAC may be administered to fish to minimize toxicity in fish parasiticide use.
참고문헌 (39)
- Adhikari S, "Effects of cypermethrin and carbofuran on haem atological parameters and prediction of their recovery in a freshwater teleost, Labeo rohita (Hamilton)", Ecotoxicol Environ Saf, 58, pp.220~226 (2004)
- Atkuri KR, "N-acetylcysteine - a safe antidote for cysteine", glutat hione deficiency, 2007, pp.7, 355~359
- Aruoma OI, "The antioxidant action of N-acetylcysteine: its reaction with hydrogen peroxide, hydroxy radical, superoxide, and hychlorous acid", Free Rad Biol Med, 6, pp.593~597 (1989)
- Bagchi D, "In vitro and in vivo generation of reactive oxygen species, DNA damage and lactate dehydrogenase leakage by selected pesticides", Toxicology, 104, pp.129~140 (1995)
- Bakker J, "Effects of N-acetylcysteine in endotoxic shock", J Crit Care, 9, pp.236~243 (1994)
- Banaclocha MM, "Therapeutic potential of N-acetylcyste ine in age-related mitochondrial neurodegenerative diseas es", Med Hypoth, 56, pp.472~477 (2001)
- Bucher F, "Effects of domestic wasterwater on serum enzyme activities of brown trout (Salmo trutta )", Comp Biochem Phsiol, C97, pp.381~385 (1990)
- Burridge L, "Chemical use in salmon aquaculture: a review of current practices and possible environmental effects", Aquaculture, 306, pp.7~23 (2010)
- Carvalho CS, "Effect of temperature on copper toxicity and hematological responses in the neotrophical fish, Prochilodus scofa at low and high pH", Aquaculture, 251, pp.109~117 (2006)
- Chatterjee A, "Genotoxic effect of arecoline given either by the peritoneal or oral route in murine bone marrow cells and the influence of N-acetylcysteine", Cancer Lett, 139, pp.23~31 (1999)
- Demael A, "Some effects of dichlorfon on carp metabolism", Comp Biochem Physiol, C95, pp.237~240 (1990)
- Denyer SP, "Mechanisms of action of antibacterial biocides", Int Biodet Biodeg, 35, pp.227~245 (1995)
- Denyer SP, "Mechanisms of action of disinfectants", Int Biodet Biodeg, 4, pp.261~268 (1998)
- "Guide Book for Aquacultural Drug Use", pp.1~61 (2010), Department of Pathology, National Fisheries Research and Development Institute, Korean Ministry of Agriculture, Fisheries and Forestry
- Hai DQ, "Organophosphate effects on antioxidant system of carp (Cyprinus carpio ) and catfish (Ictalurus nebulosus )", Comp Biochem Physiol, 117C, pp.83~88 (1997)
- Hoffer E, "N-acetylcysteine increases the glutathione content and protects rat alveolar type II cells against paraquat-induced cytotoxicity", Toxicol Lett, 84, pp.7~12 (1996)
- Issels RD, "Promotion of cysteine uptake and its utilization for glutathione biosynthesis induced by cysteamine and N-acetylcysteine", Biochem Pharmacol, 37, pp.881~888 (1988)
- Jung SH, "Effects opf formalin on hematological and blood chemistry in olive flounder, Paralichthys olivaceus (Temminck et Schlegel)", Aquaculture Res, 34, pp.1269~1725 (2003)
- Kelly GS, "Clinical applications of N-acetylcysteine", Alter Med Rev, 3, pp.114~127 (1998)
- Kiemer MCB, "The effects of hydrogen perox ide on gill tissues of Atlantic salmon, Salmo salar L", Aqua culture, 153, pp.181~189 (1997)
- Nieminen M, "Effects of form alin treatment on the blood composition of salmon (Salmo salar) and rainbow trout (Salmo gairdneri )", Comp Biochem Physiol, 76C, pp.265~269 (1983)
- Nussey G, "Effect of copper on blood coagulation of Oreochromis mossambicus (Cichlidae)", Comp Biochem Physiol, C111, pp.359~367 (1995)
- Peňa-Llopis S, "Fish toleran ce to organophosphate-induced oxidative stress is depende nt on the glutathione metabolism and enhanced by N-acet ylcysteine", Aquat Toxicol, 65, pp.337~360 (2003)
- Phelps DT, "Ele vation of glutathione levels in bovine pulmonary artery en dothelial cells by NAC", Am J Respir Cell Mol Biol, 7, pp.293~299 (1992)
- Prescott LF, "Treatment of paracetamol (acetpaminophen) poisoning with N-acetylcysteine", Lancet, 310, pp.432~434 (1977)
- Puerto M, "Dietary N-acetylcysteine (NAC) prevents histopathological changes in tilapias (Oreochromis niloticus ) exposed to a microcystin-producing cynoba cterial water bloom", Aquaculture, 306, pp.35~48 (2010)
- Roque A, "Physiolo gical stress responses of sea bass (Dicentrarchus labrax ) to hydrogen peroxide (H2O2) exposure", Aquaculture, 304, pp.104~107 (2010)
- Sanabria C, "Effects of comm only used disinfectants and temperature on swim bladder non-inflation in freshwater angelfish, Pterophyllum scalare (Lichtenstein)", Aquaculture, 292, pp.158~165 (2009)
- Schäperclaus W, "Therapy of fish disease. In: Fish Diseases", pp.210~296 (1992), AA Balkema
- Sevgiler Y, "Effects of Nacetylcysteine on oxidative responses in the liver of fenithion exposed Cyprinus carpio", Pest Biochem Physiol, 87, pp.248~254 (2007)
- Sevgiler Y, "Tissue-specific effects of fenthion on glutathione metabolism modulated by NAC and BSO in Oreochromis niloticus", Drug Chem Toxicol, 33, pp.348~356 (2010)
- Thompson RCA, "The future of veterinary parasitology: a time for change", Vet Parasitol, 98, pp.41~50 (2001)
- Treves-Brown KM, "Applied Fish Pharmacology", pp.161~219 (2000), Kluwer Academic Publishers
- Van Vuren JH., "The effect of toxicants on the hematology of Labeo umbratus (Teleostei: Cyprinidae)", Comp Biochem Physiol, C83, pp.155~159 (1986)
- Verma SR, "Isolated and combined effects of pesticides on serum transaminases in Mystus vittatus (African catfish)", Toxicol Lett, 8, pp.67~71 (1981)
- Verma RS, "Pesticideinduced dysfunction in carbohydrate metabolism in three freshwater fishes", Environ Res, 32, pp.127~133 (1983)
- Victor VM, "Regulation of macrophage function by the antioxidant N-acetylcysteine in mouse-oxidative stress by endotoxin", Int Immunopharm acol, 3, pp.97~106 (2003)
- Clinical methods for the assessment of the effects of environmental stress on fish health, "Wedemeyer G", 1018~1033, pp.U.S. Fish Wildlife Service, 89 (1977)
- Wroblewski F, "Serum glutamicoxaloacetic-transaminase activity as an index of liver cell injury from cancer: a preliminary report", Cancer, 8, pp.1155~1163 (1995)