해양퇴적층에서 적조생물(Cochlodinium polykrikoides )의 혐기성 분해과정 중 황토가 휘발성 지방산 생성에 미치는 영향
Effects of Yellow Clay on the Production of Volatile Fatty Acids during the Anaerobic Decomposition of the Red Tide Dinoflagellate Cochlodinium polykrikoides in Marine Sediments
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The formation of volatile fatty acids(VFAs) and changes in pH, oxidation and reduction potential(Eh) and acid volatile sulfide(AVS) with the addition of yellow clay were investigated using microcosm systems to examine the effects of yellow clay dispersion on the anaerobic decomposition of Cochlodinium polykrikoides in marine sediments. The acetate concentration reached a maximum by day 4 and was 1.2-1.8 fold less in the sample treated with yellow clay compared to the untreated sample (224-270 vs. 333 uM). The formate concentration reached a maximum by day 1 and was 1.3-2.8 fold less in the sample treated with yellow clay compared to the untreated sample (202-439 vs. 563 uM). The propionate concentration reached a maximum by day 2 and was 1.5-1.8 fold less in the sample treated with yellow clay compared to the untreated sample (32.6 vs. 57.2 uM). After the amounts of acetate, formate and propionate peaked the levels dropped dramatically due to the utilization by sulfate reducing bacteria. The Eh of the samples treated with yellow clay was similar to the untreated sample on day 0 but was higher in the sample treated with yellow clay(140-206 mV) from days 4 to 17. AVS started to form on day 3 and this was sustained until day 6, and 1.2-2.2 fold less was produced in the sample treated with yellow clay compared to the untreated sample (40.2-69.3 vs. 83.2-93.8 mg/L). Accordingly, during the anaerobic decomposition of C. polykrikoides in marine sediments, yellow clay dispersal seems to suppress the reduction state of Eh and the formation of volatile fatty acids(acetate, formate and propionate) used as an energy source by sulfate reducing bacteria, indicating that this process controls the production of hydrogen sulfide that negatively affects marine organisms and the marine sediment environment.
Abstract
The formation of volatile fatty acids(VFAs) and changes in pH, oxidation and reduction potential(Eh) and acid volatile sulfide(AVS) with the addition of yellow clay were investigated using microcosm systems to examine the effects of yellow clay dispersion on the anaerobic decomposition of Cochlodinium polykrikoides in marine sediments. The acetate concentration reached a maximum by day 4 and was 1.2-1.8 fold less in the sample treated with yellow clay compared to the untreated sample (224-270 vs. 333 uM). The formate concentration reached a maximum by day 1 and was 1.3-2.8 fold less in the sample treated with yellow clay compared to the untreated sample (202-439 vs. 563 uM). The propionate concentration reached a maximum by day 2 and was 1.5-1.8 fold less in the sample treated with yellow clay compared to the untreated sample (32.6 vs. 57.2 uM). After the amounts of acetate, formate and propionate peaked the levels dropped dramatically due to the utilization by sulfate reducing bacteria. The Eh of the samples treated with yellow clay was similar to the untreated sample on day 0 but was higher in the sample treated with yellow clay(140-206 mV) from days 4 to 17. AVS started to form on day 3 and this was sustained until day 6, and 1.2-2.2 fold less was produced in the sample treated with yellow clay compared to the untreated sample (40.2-69.3 vs. 83.2-93.8 mg/L). Accordingly, during the anaerobic decomposition of C. polykrikoides in marine sediments, yellow clay dispersal seems to suppress the reduction state of Eh and the formation of volatile fatty acids(acetate, formate and propionate) used as an energy source by sulfate reducing bacteria, indicating that this process controls the production of hydrogen sulfide that negatively affects marine organisms and the marine sediment environment.
참고문헌 (47)
- Bae HM, "Control of the red tide by yellow clay dispersion", In: Proceedings of Korea-China Joint Symposium on Harmful Algal Blooms, pp.53~60 (1998), Gooduck Press
- Balba MT, "Microbial metabolism of acetate, propionate and butyrate in anoxic sediment from the Colne Point Saltmarsh", Essex UK J of Gener Microbiol, 128, pp.1414~1422 (1982)
- Banat IM, "Evidence for the existence of two distinct functional groups of sulfate-reducing bacteria in salt marsh desiment", Appl and Environ Microbiol, 42, pp.985~992 (1981)
- Bisutii I, "Determination of total organic carbon an overview of current method", Tends in Analy Chem, 23, pp.716~772 (2004)
- Boschker HTS, "Bacterial populations and processes involved in acetate and propionate consumption in anoxic brackish sediment", FEMS Microbiol Ecol, 35, pp.97~103 (2001)
- Canfield DE, "Aquatic Geomicrobiology", pp.656~ (2005), Elsevier Academic Press
- Choi HG, "Removal efficiency of Cochlodinium polykrikoides by yellow loess", Kor J Fish Soc, 31, pp.109~113 (1998)
- Finke N, "Acetate, lactate, propionate and isobutylate as electron donors for iron and sulfate reduction in Artic marine sediments Svalbarde", FEMS Microbiol Ecol, 59, pp.10~22 (2007)
- Guillard RRI, "Studies of marine planktonic diatoms 1.Cyclotella nana HUSTEDT and Detonula confervacea (CLEVE) GRAN", Can J. Microbiol, 8, pp.229~239 (1962)
- Hwang JY, "Characteristics and application of Macbansuk and Hwangto", In : Proceedings of the 10 years anniversity symposium, pp.89~98 (1997), The Miner Soc Korea
- Hwang JY, "Mineralogy and chemical composition of the residualsoils (Hwangto) from South Korea", J Miner Soc Korea, 13, pp.147~163 (2000)
- Jørgensen BB, "Mineralization of organic matter in the sea bed-the role of sulphate reductions", Nature, 296, pp.643~645 (1982)
- Jørgensen BB, "Processes at the sediments-water interface. In : The major biogeochemical cycles and their interactions", pp.477~515 (1983), SCOPE Wiley
- Jørgensen BB, "Anaerobic mineralization in marine sediments from the Baltic Sea- North Sea transition", Mar Eco Prog Ser, 59, pp.39~54 (1990)
- 김경홍, "해양 퇴적물내 총탄소 및 유기탄소의 분석기법 고찰", 한국해양환경•에너지학회지, 9(4), pp.235~242 (2006), 한국해양환경·에너지학회
- Kim SJ., "Removal of red tide organisms: 2. Flocculation of red tide organisms by using loess", J Kor Fish Soc, 33, pp.455~462 (2000)
- Kim PJ, "Adsorption and removal mechanism of dissolved inorganic nutrients in seawater by Yellow loess", J Kor Fish Soc, 35, pp.146~154 (2002)
- Kondo R, "Low molecular fatty acids in the marine sediment of eutrophic coastal regions", Nippon Suisan Gakkaishi, 56, pp.519~523 (1990)
- Kondo R, "Acetate-oxidizing sulfate reduction in anoxic marine sediment", Bull Jap Soc Microb Ecol, 5, pp.29~35 (1990)
- Kondo R, "Mineralization process of glucose and low molecular fatty acid production in an anoxic marine sediment slurry", Nippon Suisan Gakkaishi, 59, pp.105~109 (1993)
- Kondo R, "Effect of temperature on the production of low molecular fatty acids within an anoxic marine sediment slurry", Nippon Suisan Gakkaishi, 59, pp.1189~1194 (1993)
- Laanbroek HJ, "Oxidation of short chain fatty acids by sulfate reducing bacteria in freshwater and in marine sediments", Arch Microbiol, 128, pp.330~335 (1981)
- Molongoski JJ, "High-performance liquid chromatography of short- and long- chain fatty acids from seawater", Applied and Environ Microbiol, 50, pp.1112~1114 (1985)
- Mueller-Harvey I, "Measurement of volatile fatty acids in pore water from marine sediments by HPLC", Est Coast Shelf Sci, 25, pp.567~579 (1987)
- Na GH, "A study on red tide control with clay suspension", J Aquaculture, 9, pp.239~245 (1996)
- Nita T, "Guideline for the remediation of the bottom sediment environment", Japan Fisheries Resource Conservation Association, 110~ (1985)
- Oremland RS, "Sulfate reduction and methanogensis in marine sediments", Geochim Cosmochim Acta, 42, pp.209~214 (1978)
- 박치현, "적조 방제용 황토살포가 연안 저서생태계에 미치는 영향", 한국환경과학회지, 15(11), pp.1035~1043 (2006), 한국환경과학회
- Park YT, "Effects of chemical composition and temperature for the production of volatile fatty acids during anaerobic decomposition process of marine sinking particles", J Korean Fish Soc, 29, pp.888~892 (1996)
- Park YT, "Study of marine sulfate-reducing bacterial population using fluorescent in situ hybridization method during decomposition processes of detrial material and polypepton in microcosm", Fish Sci, 63, pp.99~104 (1997)
- Park YT, "Changes of bacterial population during the decomposition process of red tide dinoflagellate, Cochlodinium polykrikoides in the marine sediment addition of yellow loess", J Korean Fish Sci, 31, pp.920~926 (1998)
- Parkes RJ, "Analysis of volatile fatty acids by ion-exclusion chromatography, with special reference to marine pore water", Marine Biology, 77, pp.113~118 (1983)
- Postgate JR., "The sulfate reducing bacteria, 2nd ed", pp.208~ (1984), Canbridge University Press
- Sansone FJ, "Volatile fatty acids cycling in organic rich marine sediments", Geochim Cosmochim Acta, 46, pp.1575~1589 (1982)
- Seo KS, "Effect of yellow clay on respiration and phytoplankton uptake of bivalves", Fish Sci, 74, pp.120~127 (2008)
- Shirota A., "Meeting on control of red tide with clay", Seikai Reg Fish Res Lab, 24, pp.2~5 (1976)
- Shirota A., "Red tide problem and countermeasures (2)", Int J Aquat Fish Technol, 1, pp.195~223 (1989)
- Shiozawa T, "The Characteristics of sediment in the Seto Inland Sea", Rep Gov Ind Res Inst Chugoku, 4, pp.1~24 (1979)
- Skyring GW., "Acetate as the main energy substrate for the sulfate-reducing bacteria in Lake Eliza(South Australia) hypersaline sediments", FEMS Microbiol Ecol, 53, pp.87~94 (1988)
- Sørensen FJ, "Volatile fatty acids and hydrogen as substrates for sulfate reducing bacteria in anaerobic marine sediment. Applied and Environ", Microbiol, 42, pp.5~11 (1981)
- Taylor J, "Identifying different populations of sulfate-reducing bacteria within marine sediment systems, using fatty acid biomarkers", J Gen Microbiol, 131, pp.631~642 (1984)
- Tor JM, "Metabolism of organic compounds in anaerobic, hydrothermal sulfate reducing marine sediments", Environ Microbiol, 5, pp.583~591 (2003)
- Valdemarsen T, "Degradation of organic monomers and short-chain fatty acids in sandy marine sediment by fermentation and sulfate reduction", Geochim Cosmochim Acta, 74, pp.1593~1605 (2010)
- Winfrey MR, "Substrates for sulfate reducing and methane production in intertidal sediments", Applied and Environ Microbiol, 45, pp.193~199 (1983)
- Yu Z, "Flocculation and removal of the brown tide organism, Aureococcus anophagefferens (Chrysophyceae),using clays", J Applied Phycol, 16, pp.101~110 (2004)
- Zeikus JG, "Metabolic commucation between biodegradative populations in nature. In : Microbes in their natural environments", pp.423~462 (1983), Cambridge Uni Press
- Shaw DJ, "Biogeochemistry of acetate in anoxic sediment of Skan Bay, Alaska", Geochim Cosmochim Acta, 48, pp.1819~1825 (1984)