구리에 노출된 돌돔(Oplegnathus fasciatus)의 항산화 효소 및 acetylcholinesterase 활성의 변화
Chronic Effects of Copper on Antioxidant Enzymes and Acetylcholinesterase Activities in Rock bream Oplegnathus fasciatus
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초록
A laboratory experiment was conducted to determine chronic effects of waterborne copper exposure on rock bream Oplegnathus fasciatus using a panel of enzymes. The activities of the following biochemical biomarkers were determined at different concentrations of CuSO4 for 10 and 20 days: alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) in plasma; antioxidant enzymes including glutathione (GSH), superoxide dismutase (SOD) and glutathione peroxidase (GPx) in liver and gills; and acethylcholinesterase (AChE) in brain and muscle. After exposure to two CuSO4 concentrations (200 and 400 μg/L), the activities of plasma ALT in the fish showed a tendency to increase with AST and LDH, depending on CuSO4 concentration. Additionally, GSH levels and SOD activities significantly increased, depending on CuSO4 concentrations in liver and gills. This involved the inactivation of reactive molecules formed during oxidative stress, which could provide protection against oxidative damage induced by CuSO4. However, GPx and AChE activities significantly decreased with CuSO4 in liver and gills. In conclusion, these enzymes may represent convenient biomarkers for monitoring heavy metal pollution in coastal areas. Such chronic exposure studies are necessary for improving our understanding of complementary or deleterious effects of pollutants, and for developing metal toxicity biomarkers.
Abstract
A laboratory experiment was conducted to determine chronic effects of waterborne copper exposure on rock bream Oplegnathus fasciatus using a panel of enzymes. The activities of the following biochemical biomarkers were determined at different concentrations of CuSO4 for 10 and 20 days: alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) in plasma; antioxidant enzymes including glutathione (GSH), superoxide dismutase (SOD) and glutathione peroxidase (GPx) in liver and gills; and acethylcholinesterase (AChE) in brain and muscle. After exposure to two CuSO4 concentrations (200 and 400 μg/L), the activities of plasma ALT in the fish showed a tendency to increase with AST and LDH, depending on CuSO4 concentration. Additionally, GSH levels and SOD activities significantly increased, depending on CuSO4 concentrations in liver and gills. This involved the inactivation of reactive molecules formed during oxidative stress, which could provide protection against oxidative damage induced by CuSO4. However, GPx and AChE activities significantly decreased with CuSO4 in liver and gills. In conclusion, these enzymes may represent convenient biomarkers for monitoring heavy metal pollution in coastal areas. Such chronic exposure studies are necessary for improving our understanding of complementary or deleterious effects of pollutants, and for developing metal toxicity biomarkers.
참고문헌 (60)
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