ICR 마우스를 이용한 자음강화탕 및 자음강화탕 발효물의 단회투여 독성시험
Single Oral Dose Toxicity Study of Jaeumganghwa-tang (Ziyinjianghuo-tang) and Fermented Jaeumganghwa-tang (Ziyinjianghuo-tang) in ICR Mice
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Objectives : This study aimed to evaluate the single oral dose toxicity of Jaeumganghwa-tang (Ziyinjianghuo-tang) and Jaeumganghwa-tang (Ziyinjianghuo-tang) fermented with Lactobacillus acidophyllus in male and female ICR mice. Methods : In this single oral toxicity study, non-fermented and fermented Jaeumganghwa-tang (Ziyinjianghuo-tang) were administered to male and female ICR mice as an oral dose of 500, 1000 and 2000 mg/kg. After single administration, body weight changes, general behavior, adverse effects and mortality were determined for 14 days. Serum chemistry, organ weights and necropsy findings were evaluated at the end of the experiment. Results : There were no mortality or signs of toxicity for 14 days. There were also no significant differences in body weights, organ weights, or serum chemistry values between treatment and control groups. Conclusions : These results indicate that the 50% lethal dose of Jaeumganghwa-tang (Ziyinjianghuo-tang) fermented with Lactobacillus acipophullus may be over 2000 mg/kg. This finding can be expected to provide scientific evidence for the safety of Jaeumganghwa-tang (Ziyinjianghuo-tang) fermented with Lactobacillus acidophyllus.
Abstract
Objectives : This study aimed to evaluate the single oral dose toxicity of Jaeumganghwa-tang (Ziyinjianghuo-tang) and Jaeumganghwa-tang (Ziyinjianghuo-tang) fermented with Lactobacillus acidophyllus in male and female ICR mice. Methods : In this single oral toxicity study, non-fermented and fermented Jaeumganghwa-tang (Ziyinjianghuo-tang) were administered to male and female ICR mice as an oral dose of 500, 1000 and 2000 mg/kg. After single administration, body weight changes, general behavior, adverse effects and mortality were determined for 14 days. Serum chemistry, organ weights and necropsy findings were evaluated at the end of the experiment. Results : There were no mortality or signs of toxicity for 14 days. There were also no significant differences in body weights, organ weights, or serum chemistry values between treatment and control groups. Conclusions : These results indicate that the 50% lethal dose of Jaeumganghwa-tang (Ziyinjianghuo-tang) fermented with Lactobacillus acipophullus may be over 2000 mg/kg. This finding can be expected to provide scientific evidence for the safety of Jaeumganghwa-tang (Ziyinjianghuo-tang) fermented with Lactobacillus acidophyllus.
참고문헌 (21)
- 공정현, "만병회춘", pp.204~205 (1975), 행림서원
- 조기호, "한방처방의 동서의학적 해석방법론", pp.430~ (1999), 고려의학
- 조연붕, "자음강화탕의 해열진통항경련및 O3로 중독된 백서 폐손상에 미치는 영향", 경희한의대논문집, 9, pp.475~486 (1986)
- 정희재, "자음강화탕 및 자음강화탕가미방이 실험적폐손상과 지혈에 미치는 영향", 경희한의대논문집, 15, pp.373~383 (1992)
- 김유경, "자음강화탕의 알레르기성 영증반응억제 효과", 대한한방내과학회지, 25(2), pp.174~182 (2004), 대한한방내과학회
- 정다영, "대표적인 補陰之劑의 면역 활성화 비교 연구- 육미지황탕, 자음강화탕, 쌍화탕 -", 대한한의학회지, 31(5), pp.112~123 (2010), 대한한의학회
- 김동현, "성분대사연구", 최신고려인삼연구, 1, pp.71~88 (2007)
- 최윤경, "발효한약의 최근 연구 동향 - 안전성과 유효성 기반", 생명과학회지, 22(12), pp.1729~1739 (2012), 한국생명과학회[DOI]
- 김은아, "유산균에 의한 Escherichia coli 와 Salmonella typhimurium의 생육억제에 관한 연구", 한국동물자원과학회지, 44(4), pp.491~498 (2002), 한국동물자원과학회
- White JA, "An Evaluation of the Waters Pico-Tagsystem for the amino-acid analysis of food materials", J Autom Chem, 8(4), pp.170~177 (1986)
- 김종대, "補中益氣湯의 마우스 경구 단회 투여 독성시험", 대한예방한의학회지, 15(2), pp.21~38 (2011), 대한예방한의학회
- 식품의약품안전청, "의약품등의 독성시험기준: 식품의약품안전청 고시2009-116호", 식품의약품안전청, ~ (2009)
- OECD, "OECD guidelines for the testing of chemicals No. 425 Acute oral toxicity", ~ (2008)
- Hayes AW, "Hayes Toxicology", pp.17~19 (1984), Raven press
- 정용준, "발효한약추출물 HP-1이 알코올을 투여한 쥐의 알코올 대사에 미치는 영향", 동의생리병리학회지, 21(2), pp.387~391 (2007), 대한동의병리학회
- 강동희, "유산발효에 의한 발효한약의 기능분석", 한국미생물·생명공학회지, 39(3), pp.259~265 (2011), 한국미생물·생명공학회
- 박진한, "4종의 발효균주가 당귀의 nodakenin과 decursin의 함량에 미치는 변화", 대한본초학회지, 25(4), pp.7~10 (2010), 대한본초학회
- Eum HA, "Protective Effect of Ssanghwa-Tang Fermented by Lactobacillus Fermentum Against Carbon Tetrachloride-Induced Acute Hepatotoxicity in Rats", Afr J Tradit Complement Altern Med, 8(3), pp.312~321 (2011)
- Chung TH, "Effectiveness of the Novel Herbal Medicine, KIOM-MA, and Its Bioconversion Product, KIOM-MA128, on the Treatment of Atopic Dermatitis", Evid Based Complement Alternat Med, 2012, pp.762918~ (2012)
- Kobashi K, "Relation of Intestinal Bacteria to Pharmacological Effects of Glycosides", Biosci Microflora, 16, pp.1~7 (1997)
- 식품의약품안전청, "의약품 임상시험계획 승인지침: 식품의약품안전청고시 제2010-63호", 식품의약품안전청, ~ (2010)