木香이 고지방 식이에 의한 비만으로 유발된 인슐린 저항성 mouse의 염증 및 인슐린 저항성에 미치는 영향
Effects of Inula helenium on Inflammation and Insulin Resistance in Obesity-induced Insulin Resistance Mouse by High Fat Diet
2
조회
0
다운로드
4
인용
0
참고문헌
키워드
초록
Objectives: Obesity is an important cause of insulin resistance that leads to obese type 2 diabetes. Recently it has been found that obesity is associated with adipose tissue accumulation which causes systemic inflammation. In this study, we investigated effects of Inula helenium on the inflammation in high fat diet-induced insulin resistance mouse. Methods: Insulin resistance was induced in C57BL/6 male mice (19~21 g) on a 60% fat diet. Mice were divided into 3 groups (n=6) of normal, control and Inula helenium. After 12 weeks, body weight, FBS, oral glucose tolerance test (OGTT), serum level of insulin, epididymal fat pad, liver weight and the gene expression of tumor necrosis factor (TNF)-α, interleukin (IL)-6, interleukin (IL)-10 and cluster of differentiation (CD) 68 were measured. Also, adipose tissue macrophage was analyzed by fluorescence activated cell sorting. Results: Inula helenium significantly reduces oral glucose tolerance levels, insulin serum level and adipose tissue macrophage. Also Inula helenium increased IL-10 gene expression and decreased CD68 gene expression. Conclusions: These results show that Inula helenium has anti-insulin resistance and anti-inflammatory effects on a high fat diet-induced insulin resistance mouse model.
Abstract
Objectives: Obesity is an important cause of insulin resistance that leads to obese type 2 diabetes. Recently it has been found that obesity is associated with adipose tissue accumulation which causes systemic inflammation. In this study, we investigated effects of Inula helenium on the inflammation in high fat diet-induced insulin resistance mouse. Methods: Insulin resistance was induced in C57BL/6 male mice (19~21 g) on a 60% fat diet. Mice were divided into 3 groups (n=6) of normal, control and Inula helenium. After 12 weeks, body weight, FBS, oral glucose tolerance test (OGTT), serum level of insulin, epididymal fat pad, liver weight and the gene expression of tumor necrosis factor (TNF)-α, interleukin (IL)-6, interleukin (IL)-10 and cluster of differentiation (CD) 68 were measured. Also, adipose tissue macrophage was analyzed by fluorescence activated cell sorting. Results: Inula helenium significantly reduces oral glucose tolerance levels, insulin serum level and adipose tissue macrophage. Also Inula helenium increased IL-10 gene expression and decreased CD68 gene expression. Conclusions: These results show that Inula helenium has anti-insulin resistance and anti-inflammatory effects on a high fat diet-induced insulin resistance mouse model.
참고문헌 (30)
- 대한내과학회 해리슨내과학 편집위원회, "HARRISON'S 내과학", pp.2744~ (2010), MIP
- 대한비만학회, "임상비만학", pp.143~167 (2008), 고려의학
- 대한당뇨병학회, "당뇨병학", pp.310~ (2005), 고려의학
- 대한내분비학회, "내분비대사학 (Textbook of Endocrinology and Metabolism) -2nd edition-", pp.639~640 (2011), 군자출판사
- 박용우, "비만치료의 최신지견", pp.49~107 (2007), 한미의학
- Mohamed-Ali V, "Adipose tissue as an endocrine and paracrine organ", Int J Obes (Lond), 22, pp.1145~1158 (1998)
- Weisberg SP, "Obesity is associated with macrophage accumulation in adipose tissue", J Clin Invest, 112, pp.1796~1808 (2003)
- 전국 한의과대학 신계내과학교실, "신계내과학", pp.89~91 (2011), 군자출판사
- 윤창렬, "黃帝內經素問 王氷注(中)", pp.231~232 (2003), 주민출판사
- 朱震亨, "丹溪醫集", pp.324~ (1995), 인민위생출판사
- 張介賓, "景岳全書 雜證謨(上)", pp.836~ (2006), 한미의학
- 대성문화사편집부, "欽定 四庫全書 子部 五醫家類", pp.527~528 (1995), 대성문화사
- 한의과대학 본초학 편찬위원회, "본초학", pp.395~ (2012), 영림사
- 이영준, "A549세포에 대한 목향추출물의 ROS 매개 세포독성", 대한예방한의학회지, 17(2), pp.169~178 (2013), 대한예방한의학회
- 김대성, "목향에탄올추출물의 ROS-MAPKs 경로를 통한 세포사멸 유도", 약 학 회 지, 56(3), pp.173~179 (2012), 대한약학회[DOI]
- 오희라, "木香과 車前草가 胃癌細胞의 活性, 增殖, 自己殺害能 및 세포주기관련 유전자 발현에 미치는 영향", 대한한방종양학회지, 7, pp.1~18 (2001)
- 윤태숙, "Anti-obesity Activity of Extract from Saussurea lappa", 한국약용작물학회지, 18(3), pp.151~156 (2010), 한국약용작물학회
- 이명수, "파골세포 분화에 목향 물 추출물의 효과", 동의생리병리학회지, 25(3), pp.516~520 (2011), 대한동의병리학회
- 이민석, "설치류 대식세포에서 木香 추출물의 항염증 효과", 동의생리병리학회지, 25(2), pp.275~279 (2011), 대한동의병리학회
- Kahn BB, "Obesity and insulin resistance", J Clin Invest, 106(4), pp.473~481 (2000)
- 전국의과대학교수, "오늘의 진단 및 치료", pp.1252~ (2000), 한우리
- 권오준, "길경 투여가 고지방, 고탄수화물 식이로 유발된 비만형 제2형 당뇨병 동물모델에 미치는 영향", 대한한의학회지, 34(1), pp.1~14 (2013), 대한한의학회
- Maeda N, "Diet-induced insulin resistance in mice lacking adiponectin", ACRP30, 2002, pp.8(7), 731~737
- 백선호, "神麯 투여가 비만형 제 2형 당뇨병 동물모델의 대사인자와 지방조직 염증반응에 미치는 영향", 대한한의학회지, 33(3), pp.33~45 (2012), 대한한의학회
- Hotamisligil GS, "Increased adipose tissue expression of tumor necrosis factor-α in human obesity and insulin resistance", J Clin. Invest, 95, pp.2409~2415 (1995)
- Hotamisligil GS, "IRS-1 mediated inhibition of insulin receptor tyrosine kinase activity in TNF-alpha and obesity-induced insulin resistance", Science, 271(5249), pp.665~668 (1996)
- Fantuzzi G, "Adipose tissue, adipokines and inflammation", J Allergy Clin Immunol, 115, pp.911~919 (2005)
- Yilma AN, "The anti-inflammatory cytokine, interleukin-10, inhibits inflammatory mediators in human epithelial cells and mouse macrophages exposed to live and UV-inactivated Chlamydia trachomatis", Mediators Inflamm, 2012, pp.1~10 (2012)
- Bogdan C, "Macrophage deactivation by interleukin 10", J Exp Med, 174(1), pp.1549~1555 (1991)
- Di Gregorio GB, "Expression of CD68 and macrophage chemoattractant protein-1genes in human adipose and muslce tissues", Diabetes, 54(8), pp.2305~2313 (2005)