Comparision of antioxidant and anti-inflammatory activities of enzyme assisted hydrolysate from Ecklonia maxima blades and stipe
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Marine brown seaweeds are a source of functional ingredients with various biological properties. They have been used in the food and functional food industries. Brown seaweeds are divided into three parts of blades, stipe, and root. Normally seaweed blades were used as raw materials for biological research. However, there are limited uses on stipes of Ecklonia maxima (E. maxima) depending on the physicochemical, nutritional, and biological properties. Besides, the comparative studies of two structures of E. maxima, blades and stipe didn’t discover previously. This study aimed to compare the potent antioxidant and anti- inflammatory activities of the two structures of E. maxima, blades and stipe in vitro studies to increase the utilization of the two structures of E. maxima. The enzyme-assisted hydrolysate from E. maxima showed significant antioxidant and anti-inflammatory activities. Among them, celluclast-assisted hydrolysate from E. maxima blades (EMBC) and viscozyme-assisted hydrolysate from E. maxima stipe (EMSV) expressed significant protection on hydrogen peroxide-induced oxidative stress. Moreover, EMBC and EMSV treatment remarkably reduced nitric oxide production by downregulation of pro-inflammatory cytokine expressions in lipopolysaccharide-stimulated Raw 264.7 cells. Especially EMBC showed strong inhibition on pro-inflammatory cytokine production compared to EMSV. Taken together research findings suggest that EMBC and EMSV possessed potent antioxidant and anti-inflammatory properties and may be utilized as functional ingredients in the food and functional food sectors.
Abstract
Marine brown seaweeds are a source of functional ingredients with various biological properties. They have been used in the food and functional food industries. Brown seaweeds are divided into three parts of blades, stipe, and root. Normally seaweed blades were used as raw materials for biological research. However, there are limited uses on stipes of Ecklonia maxima (E. maxima) depending on the physicochemical, nutritional, and biological properties. Besides, the comparative studies of two structures of E. maxima, blades and stipe didn’t discover previously. This study aimed to compare the potent antioxidant and anti- inflammatory activities of the two structures of E. maxima, blades and stipe in vitro studies to increase the utilization of the two structures of E. maxima. The enzyme-assisted hydrolysate from E. maxima showed significant antioxidant and anti-inflammatory activities. Among them, celluclast-assisted hydrolysate from E. maxima blades (EMBC) and viscozyme-assisted hydrolysate from E. maxima stipe (EMSV) expressed significant protection on hydrogen peroxide-induced oxidative stress. Moreover, EMBC and EMSV treatment remarkably reduced nitric oxide production by downregulation of pro-inflammatory cytokine expressions in lipopolysaccharide-stimulated Raw 264.7 cells. Especially EMBC showed strong inhibition on pro-inflammatory cytokine production compared to EMSV. Taken together research findings suggest that EMBC and EMSV possessed potent antioxidant and anti-inflammatory properties and may be utilized as functional ingredients in the food and functional food sectors.
참고문헌 (47)
- AOAC, "Official methods of analysis. 17th ed", Association of Official Analytical Chemists, ~ (1999)
- Anderson RJ, "Holdfasts of adult kelp Ecklonia maxima provide refuges from grazing for recruitment of juvenile kelps", Mar Ecol Prog Ser, 159, pp.265~273 (1997)
- Bolton JJ, "South African kelp moving eastwards : the discovery of Ecklonia maxima(Osbeck)Papenfuss at De Hoop Nature Reserve on the south coast of South Africa", Afr J Mar Sci, 34, pp.147~151 (2012)
- Bolton JJ, "Light and temperature requirements for growth and reproduction in gametophytes of Ecklonia maxima(Alariaceae : Laminariales)", Mar Biol, 87, pp.131~135 (1985)
- Cardoso SM, "Seaweeds as preventive agents for cardiovascular diseases : from nutrients to functional foods", Mar Drugs, 13, pp.6838~6865 (2015)
- Castro LSEPW, "Fucose-containing sulfated polysaccharides from brown macroalgae Lobophora variegata with antioxidant, anti-inflammatory, and antitumoral effects", J Appl Phycol, 26, pp.1783~1790 (2014)
- Carroll AR, "Marine natural products", Nat Prod Rep, 37, pp.175~223 (2020)
- Chandler SF, "The effect of phosphate, nitrogen and sucrose on the production of phenolics and solasodine in callus cultures of Solanum laciniatum", Plant Cell Rep, 2, pp.205~208 (1983)
- Chen Z, "Role of oxidative stress in the pathogenesis of nonalcoholic fatty liver disease", Free Radic Biol Med, 152, pp.116~141 (2020)
- Ciepiela GA, "The effect of seaweed Ecklonia maxima extract and mineral nitrogen on fodder grass chemical composition", Environ Sci Pollut Res, 23, pp.2301~2307 (2016)
- Dodgson KS, "A note on the determination of the ester sulphate content of sulphated polysaccharides", Biochem J, 84, pp.106~110 (1962)
- Dubois M, "Colorimetric method for determination of sugars and related substances", Anal Chem, 28, pp.350~356 (1956)
- Featonby-Smith BC, "Identification and seasonal variation of endogenous cytokinins in Ecklonia maxima(Osbeck)Papenf", Bot Mar, 27, pp.527~530 (1984)
- Feng D, "Adsorption of heavy metals by biomaterials derived from the marine alga Ecklonia maxima", Hydrometallurgy, 73, pp.1~10 (2004)
- I.P. Shanura Fernando, "FTIR characterization and antioxidant activity of water soluble crude polysaccharides of Sri Lankan marine algae", ALGAE, 32(1), pp.75~86 (2017), 한국조류학회I[DOI]
- I.P. Shanura Fernando, "Antioxidant Activity of Marine Algal Polyphenolic Compounds: A Mechanistic Approach", Journal of Medicinal Food, 19(7), pp.615~628 (2016), 한국식품영양과학회[DOI]
- Gyurászová M, "Oxidative stress in the pathophysiology of kidney disease : implica tions for noninvasive monitoring and identification of biomarkers", Oxid Med Cell Longev, 2020, pp.5478708~ (2020)
- Kannan RRR, "Acetylcholinesterase inhibitory activity of phlorotannins isolated from the brown alga, Ecklonia maxima(Osbeck)Papenfuss", Food Res Int, 54, pp.1250~1254 (2013)
- Kang MC, "Antioxidant properties of a sulfated polysaccharide isolated from an enzymatic digest of Sargassum thunbergii", Int J Biol Macromol, 132, pp.142~149 (2019)
- Kim EA, "Protective effect of fucoidan against AAPH-induced oxidative stress in zebrafish model", Carbohydr Polym, 102, pp.185~191 (2014)
- Kocira A, "Enhancement of yield, nutritional and nutraceutical properties of two common bean cultivars following the application of seaweed extract(Ecklonia maxima)", Saudi J Biol Sci, 25, pp.563~571 (2018)
- Kulkarni MG, "Bioactive molecules derived from smoke and seaweed Ecklonia maxima showing phytohormone-like activity in Spinacia oleracea L", New Biotechnol, 48, pp.83~89 (2019)
- Lee HG, "Potential antioxidant properties of enzymatic hydrolysates from Stichopus japonicus against hydrogen peroxide-induced oxidative stress", Antioxidants, 10, pp.110~ (2021)
- Lee YH, "Cytotoxicity, oxidative stress, apoptosis and the autophagic effects of silver nanoparticles in mouse embryonic fibroblasts", Biomaterials, 35, pp.4706~4715 (2014)
- Libby P, "Inflammatory mechanisms : the molecular basis of inflammation and disease", Nutr Rev, 65, pp.S140~S146 (2007)
- Lordan S, "Marine bioactives as functional food ingredients : potential to reduce the incidence of chronic diseases", Mar Drugs, 9, pp.1056~1100 (2011)
- Mayombo NAS, "Diatoms associated with two South African kelp species : Ecklonia maxima and Laminaria pallida", Afr J Mar Sci, 41, pp.221~229 (2019)
- Mwangi HM, "Isolation, identification and radical scavenging activity of phlorotannin derivatives from brown algae, Ecklonia maxima : an experimental and theoretical study", Free Radic Antioxid, 3, pp.S1~10 (2013)
- Newman DJ, "Marine natural products and related compounds in clinical and advanced preclinical trials", J Nat Prod, 67, pp.1216~1238 (2004)
- Nguyen TT, "Protein recovery from underutilised marine bioresources for product development with nutraceutical and pharmaceutical bioactivities", Mar Drugs, 18, pp.391~ (2020)
- Olasehinde TA, "Macroalgae as a valuable source of naturally occurring bioactive compounds for the treatment of Alzheimer’s disease", Mar Drugs, 17, pp.609~ (2019)
- Papenfus HB, "Seasonal variation in the polyamines of Ecklonia maxima", Bot Mar, 55, pp.539~546 (2012)
- Papenfuss GF, "Studies of South African Phaeophyceae. I. Ecklonia maxima, Laminaria pallida, Macrocystis pyrifera", Am J Bot, 29, pp.15~24 (1942)
- Peñalver R, "Seaweeds as a functional ingredient for a healthy diet", Mar Drugs, 18, pp.301~ (2020)
- Pereira F, "Have marine natural product drug discovery efforts been productive and how can we improve their efficiency", Expert Opin Drug Discov, 14, pp.717~722 (2019)
- Pereira SA, "A bioeconomic analysis of the potential of seaweed Hypnea pseudomusciformis farming to different targeted markets", Aquac Econ Manag, 24, pp.507~525 (2020)
- Podkowińska A, "Chronic kidney disease as oxidative stress-and inflammatory-mediated cardiovascular disease", Antioxidants, 9, pp.752~ (2020)
- Rekatsina M, "Pathophysiology and therapeutic perspectives of oxidative stress and neurodegenerative diseases : a narrative review", Adv Ther, 37, pp.113~139 (2020)
- Rengasamy KRR, "Potential antiradical and alpha-glucosidase inhibitors from Ecklonia maxima(Osbeck)Papenfuss", Food Chem, 141, pp.1412~1415 (2013)
- Rothman MD, "The effects of harvesting of the South African kelp (Ecklonia maxima) on kelp population structure, growth rate and recruitment", Paper presented at: 18th International Seaweed Symposium (2004)
- Rothman MD, "Geographical variation in morphology of the two dominant kelp species, Ecklonia maxima and Laminar ia pallida(Phaeophyceae, Laminariales), on the west coast of Southern Africa", J Appl Phycol, 29, pp.2627~2639 (2017)
- Rouphael Y, "Effect of Ecklonia maxima seaweed extract on yield, mineral composition, gas exchange, and leaf anatomy of zucchini squash grown under saline conditions", J Appl Phycol, 29, pp.459~470 (2017)
- Wang L, "The potential of sulfated polysaccharides isolated from the brown seaweed Ecklonia maxima in cosmetics : antioxidant, anti-melanogenesis, and photoprotective activities", Antioxidants, 9, pp.724~ (2020)
- Lei Wang, "In vitro and in vivo anti-inflammatory activities of a sterol-enriched fraction from freshwater green alga, Spirogyra sp.", Fisheries and Aquatic Sciences, 23(4), pp.1~9 (2020), 한국수산과학회
- Wang J, "Eckmaxol, a phlorotannin extracted from Ecklonia maxima, produces anti-β-amyloid oligomer neuroprotective effects possibly via directly acting on glycogen synthase kinase 3β", ACS Chem Neurosci, 9, pp.1349~1356 (2018)
- Waterborg JH, "The protein protocols handbook", pp.7~10 (2009), Humana Press
- Zhou X, "Isolation and purification of a neuroprotective phlorotannin from the marine algae Ecklonia maxima by size exclusion and high-speed counter-current chromatography", Mar Drugs, 17, pp.212~ (2019)
