본문 바로가기
KERD
논문KCI 등재

Molecular Identification of the Toxic Alexandrium tamiyavanichii (Dinophyceae) by the Whole-cell FISH Method

김창훈|김충재|Sada-Aki Yoshimatsu|Yoshihiko Sako
한국수산과학회 (The Korean Society of Fisheries and Aquatic Science)|2004.12|Fisheries and Aquatic Sciences|제7권 제4호|pp. 175-183

1

조회

0

다운로드

0

인용

0

참고문헌

주제분류수산학

초록

Abstract

The dinoflagellate Alexandrium tamiyavanichii Balech, a producer of toxins causing paralytic shellfish poisoning (PSP), has recently been considered as one of main organisms responsible for toxication of shellfish in Japan. In this study, A. tamiyavanichii was subjected to a molecular phylogenetic analysis inferred from 28S rDNA D1-D2 sequences and a species-specific LSU rRNA-targeted oligonucleotide DNA probe was designed to identify A. tamiyavanichii using the whole cell-FISH (fluorescence in situ hybridization). The sequences of the 28S rDNA D1-D2 region of A. tamiyavanichii showed no difference from A. cohorticular AF174614 (present name A. tamiyavanichii) and formed a distinct clade from the ‘tamarensis species complex’. The probe, TAMID2, reacted specifically with A. tamiyavanichii cultured cells, without any cross-reaction with other species belonging to the same genus, including A. tamarense, A. catenella, A. affine, A. fraterculus, A. insuetum and A. pseudogonyaulax. In a test of cross- reactivity with a field sample, TAMID2 reacted consistently with only A. tamiyavanichii, indicating that the present protocol involving the TAMID2 probe might be useful for detecting toxic A. tamiyavanichii in a simple and rapid manner.

참고문헌 (30)

  1. "Analysis of Alexandrium (Dinophyceae) species using sequences of the 5.8S ribosomal DNA and internal transcribed spacer regions.", 32, pp.424~432 (1996)
  2. "Identification of the toxic dinoflagellates Alexandrium catenella and A. tamarense (Dinophyceae) using DNA probes and whole cell hybridization.", 32,, pp.1049~1052 (1996)
  3. "Fluorescent oligonucleotide probing of whole cells for determi- native and environmental studies in microbiology", pp.762~770
  4. "Species-specific assessment of Mycobacterium leprae in skin biopsies by in situ hybridization and polymerase chain reaction Lab", pp.618~623 (1992)
  5. "The genus Alexandrium Halim (Dino- flagellata). Sherkin Island Marine Station,", pp.151.~ (1995), Sherkin Island, Co.
  6. "Quantitative use of fluorescent in situ hybridization to examine relationships between mycolic acid-containing actinomycetes and foaming in activated sludge plants.", 66,, pp.1158~1160 (2000)
  7. "Detection and enumeration of methano -trophs in acidic Sphagnum peat by 16S RNA fluorescence in situ hybridization, including the use of newly de- veloped oligonucleotide probes for Methylocella palustris.", 67, pp.4850~4857 (2001)
  8. "ribosomal RNA probes for clinical application", pp.41~44 (1990)
  9. "Phylogenetic characterization and in situ localization of the using 16S RNA sequence analysis and oligo- deoxynucleotide probe hybridization", pp.2376~2382 (1991)
  10. "Distance methods for inferring phy- logenies", tion, pp.16~24 (1984)
  11. "Confidence limits on phylogenies", tion, pp.783~791 (1985)
  12. "PHYLIP (Phylogeny Inference Pac- kage). Version 3.572c. Distributed by the author", pp.~3 (1995), Department of Genetics, University of Wa- shington.
  13. "Morphology of Protogonyaulax ta- marensis Taylor from Japanese coastal waters", pp.529~537 (1985)
  14. "Phylogenetic group-specific oligodeoxynu- cleotide probes for identification of single microbial cells", pp.720~726 (1988)
  15. "First paralytic shellfish poison (PSP) infestation of bivalves due to toxic dinoflagellate Alexandrium tamiya- vanichii, in the southeast coasts of the Seto Inland Sea, Japan.", 43, pp.1~5 (2001)
  16. "a toxic dinoflagellate found in the Gulf of Thailand", pp.707~712 (1988)
  17. "Permeabilization of mycolic -acid-containing actino- mycetes for in situ hybridization with fluorescently labeled oligonucleotide probes", pp.2859~2865 (1994)
  18. "Identification of cultured Pseudo-nitzschia (Bacillariophyceae) using species-specific LSU rRNA-targeted fluorescent probes.", 32, pp.646~655 (1996)
  19. "TREE VIEW: an application to display phylogenetic trees on personal computers.", 12, pp.357~358 (1996)
  20. "Pseudo-nitzschia species (Bacillariophyceae) in Louisiana coastal waters: molecular probe field trials, genetic variability, and domoic acid analyses.", 35, pp.1368~1378 (1999)
  21. "a new method for reconstructing phylogenetic trees", e, pp.406~425 (1987)
  22. "Isozyme and cross analysis of mating populations in the Alexandrium catenella", tamarense species complex, 1990, pp.ni(miya), 320~323
  23. "Molecular identification of toxic marine dinoflagellate Alexandrium species", 54, pp.311~324 (1999)
  24. "Identification of Pseudo-nitzschia australis (Bacillariophyceae) using rRNA-targeted probes in whole cell and sandwich hybridization formats.", 35,, pp.190~197
  25. "Identification of group- and strain-specific genetic markers for globally distributed Alexandrium Sequence analysis of a fragment of the LSU rRNA gene", di(phyceae), pp.999~1011 (1994)
  26. "Quantification of different Eubacterium spp. in human fecal samples with species-specific 16S rRNA-targeted oligonu- cleotide probes.", 66,, pp.375~382 (2000)
  27. "Development and application of nucleic acid probes in bacterial systematics Nucleic Acid Techniques in Bacterial Systematics", pp.205~248 (1991), John Wiley & Sons Ltd
  28. "The ClustalX windows interface: flexi- ble strategies for multiple sequence alignment aided by quality analysis tools.", 24,, pp.4876~4882 (1997)
  29. "In situ hybridization of Prochlorococcus and Synechococcus (marine cyanobacteria) spp. with rRNA-targeted pep- tide nucleic acid probes.", 66, pp.284~289 (2000)
  30. "Geographic differences in paralytic shellfish poisoning toxin profiles among Japanese populations of Alexandrium tamarense and A. catenella (Dinophyceae).", 49,, pp.13~21 (2001)

관련 논문AI추천

Sensitive, Accurate PCR Assays for Detecting Harmful Dinoflagellate Cochlodinium polykrikoides Using a Specific Oligonucleotide Primer Set김근용, 김창훈, 박기홍한국수산과학회 · Fisheries and Aquatic Sciences2004 · 제7권 제3호Population Analysis of Korean and Japanese Toxic Alexandrium catenella Using PCR Targeting the Area Downstream of the Chloroplast PsbA Gene김창훈, 김충재, Yoshihiko Sako한국수산과학회 · Fisheries and Aquatic Sciences2004 · 제7권 제3호Rapid detection and Quantification of Fish Killing Dinoflagellate Cochlodinium polykrikoides (Dinophyceae) in Environmental Samples Using Real-time PCR강양순, 김창훈, 박영태, 박태규, 서미경한국수산과학회 · Fisheries and Aquatic Sciences2008 · 제11권 제4호Morphological Re-examination of Prorocentrum spp. in Korean Coastal Waters박종규, 신은영, 여환구한국환경생물학회 · 환경생물2005 · 제23권 제2호Uptake and Excretion of Dissolved Organic Phosphorus by Two Toxic Dinoflagellates, Alexandrium tamarense Lebour(Balech) and Gymnodinium catenatum Graham오석진, 윤양호, Tamiji한국수산과학회 · Fisheries and Aquatic Sciences2006 · 제9권 제1호Detection of Heterotrophic Dinoflagellate Pfiesteria piscicida (Dinophyceae) in Surface Water Samples Using Real-time PCR강양순, 박영태, 박태규, 서미경한국수산과학회 · Fisheries and Aquatic Sciences2008 · 제11권 제4호

이 간행물의 다른 논문 (4건)

Effects of Dietary Protein and Lipid Levels on Growth, Feed Utilization and Body Composition of Adult Starry Flounder (Platichthys stellatus)이종하, 이상민, 조성환, 김경덕, 임한규pp. 184-191The Effects of Temperature, Starvation, and Kind of Foods on Growth, RNA/DNA Ratios and Protein Contents during the Early Life Stages of Olive Flounder (Paralichthys olivaceus)Masaru Tanaka, 곽우석pp. 192-203Wind Effects on the Oyster Farm Environment in Gamak Bay이문옥, 박성진pp. 204-214Monitoring of Some Chlorobenzenes in Marine Sediments and Bivalves from Several Coastal Regions of Korea이수정, 최희구, 문효방pp. 215-218
원문 보기