굴(Crassostrea gigas), 멍게(Halocynthia roretzi) 및 해삼(Apostichopus japonicus)에서 분리한 대장균의 항균제 내성 특성
Antimicrobial Resistance of Escherichia coli isolated from Oyster Crassostrea gigas, Sea Squirts Halocynthia roretzi and Sea Cucumbers Apostichopus japonicus
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This study evaluated the abundance of fecal coliforms in oysters Crassostrea gigas, sea squirts Halocynthia roretzi and sea cucumbers Apostichopus japonicus in fisheries along the coast of Korea in 2014, and investigated the prevalence of antimicrobial resistance in Escherichia coli isolated from these fishery products. The ranges of fecal coliforms found in oysters, sea squirts and sea cucumbers were <18-20, <18-330 and <18-3,300 MPN (most probable number)/100 g, respectively. Sea squirts contained the greatest range of E. coli (<20-140 MPN/100 g), followed by sea cucumbers (<20-130 MPN/100 g) and oysters (<20-20 MPN/100 g). A total of 26 strains of E. coli were isolated from 34 sea squirt, 25 sea cucumber and 13 oyster samples. Strains thus isolated were tested for their susceptibility to 22 antimicrobial agents used in Korea for medical or veterinary therapy. E. coli isolates showed the greatest resistance to ampicillin (84.6%), followed by trimethoprim (34.6%), nalidixic acid (34.6%), tetracycline (30.8%), pipemidic acid (26.9%), streptomycin (23.1%), chloramphenicol (23.1%), trimethoprim/sulfamethoxazole (23.1%), and gentamicin (15.4%). Resistance to at least one antimicrobial agent was present in 88.5% of E. coli isolates. Of the 26 isolated, six strains (23.1%) were resistant to multiple antimicrobial agents.
Abstract
This study evaluated the abundance of fecal coliforms in oysters Crassostrea gigas, sea squirts Halocynthia roretzi and sea cucumbers Apostichopus japonicus in fisheries along the coast of Korea in 2014, and investigated the prevalence of antimicrobial resistance in Escherichia coli isolated from these fishery products. The ranges of fecal coliforms found in oysters, sea squirts and sea cucumbers were <18-20, <18-330 and <18-3,300 MPN (most probable number)/100 g, respectively. Sea squirts contained the greatest range of E. coli (<20-140 MPN/100 g), followed by sea cucumbers (<20-130 MPN/100 g) and oysters (<20-20 MPN/100 g). A total of 26 strains of E. coli were isolated from 34 sea squirt, 25 sea cucumber and 13 oyster samples. Strains thus isolated were tested for their susceptibility to 22 antimicrobial agents used in Korea for medical or veterinary therapy. E. coli isolates showed the greatest resistance to ampicillin (84.6%), followed by trimethoprim (34.6%), nalidixic acid (34.6%), tetracycline (30.8%), pipemidic acid (26.9%), streptomycin (23.1%), chloramphenicol (23.1%), trimethoprim/sulfamethoxazole (23.1%), and gentamicin (15.4%). Resistance to at least one antimicrobial agent was present in 88.5% of E. coli isolates. Of the 26 isolated, six strains (23.1%) were resistant to multiple antimicrobial agents.
참고문헌 (22)
- Acar JF, "antibiotics in laboratory medicine", pp.17~52 (1991), Williams &Wilkins
- CLSI, "Performance standards for antimicrobial susceptibility testing. CLSI document M100-S22", Clinical and Laboratory Standard Institute, 44~128 (2012)
- Dennesen PJ, "Multiresistant bacteria as a hospital epidemic problem", Ann Med, 30, pp.176~185 (1998)
- European Commission, "Regulation(EC)No 854", 2004of the European Parliament and of the Council of 29 April 2004 laying down specific rules for the organization of official control on products of animal origin intended for human consumption, 2004, pp.L139, 206~321
- Feldhusen F, "The role of seafood in bacterial foodborne disease", Microbes Infect, 2, pp.1651~1660 (2000)
- Grimes DJ, "Ecology of estuarine bacteria capable of causing human disease : A review", Estuaries, 14, pp.345~360 (1991)
- Dagne Hill, "The Impact of Rainfall on Fecal Coliform Bacteria in Bayou Dorcheat (North Louisiana)", International Journal of Environmental Research and Public Health, 3(1), pp.114~117 (2006), MDPI AG[DOI]
- 조미라, "서해안 패류양식장에서 분리된 대장균의 항균제 내성", 한국수산과학회지, 49(1), pp.13~19 (2016), 한국수산과학회
- "Food balance sheet" (2015)[URL]
- 권순재, "창선 해역의 지중해담치(Mytilus galloprovincialis) 양식장 및 육상오염원에서 분리한 대장균(Escherichia coli)의 항생제 내성", 한국수산과학회지, 49(5), pp.564~572 (2016), 한국수산과학회
- 이재인, "수산물에서 분리한 대장균의 분리특성 및 항생제 감수성", 한국식품영양학회지, 16(2), pp.111~115 (2003), 한국식품영양학회
- 이태식, "패류 양식장의 세균학적 수질에 미치는 강우의 영향", 한국수산과학회지, 43(5), pp.406~414 (2010), 한국수산과학회
- Levin BR, "The population genetics of antibiotic resistance", Clin Infect Dis, 24, pp.9~16 (1997)
- Mach PA, "R-plasmid transfer in a wastewater treatment plant", Appl Environ Microbiol, 44, pp.1395~1403 (1982)
- Major statistics of oceans and fisheries. Oceans and fisheries report, "Ministry of Oceans and Fisheries", 1~510 (2015)
- 박큰바위, "강우 발생에 따른 남해군 봉천 방출수가 강진만 해역의 세균학적 수질에 미치는 영향 평가", 한국수산과학회지, 44(6), pp.622~629 (2011), 한국수산과학회
- 박큰바위, "강우 발생에 따른 육상오염원이 나로도 해역의 세균학적 수질에 미치는 영향 평가", 한국수산과학회지, 45(5), pp.414~422 (2012), 한국수산과학회
- 박큰바위, "남해안 패류양식장에서 분리된 대장균(Escherichia coli)의 항균제 내성", 한국수산과학회지, 46(5), pp.528~533 (2013), 한국수산과학회[DOI]
- Sandas RA, "Transferable drug resistance in bacteria from fish-farm sediments", Canadian J Microbiol, 38, pp.1061~1065 (1992)
- 손광태, "남해안 어류양식장에서 분리된 Escherichia coli에 대한 항균제 감수성", 한국수산과학회지, 42(4), pp.322~328 (2009), 한국수산과학회
- Song SW, "Surveillance of antimicrobial susceptibility of bacteria isolated from slaughterhouse in Korea, 2003. 1. Antimicrobial susceptibility of E. coli isolated from carcasses in slaughterhouse", Kor J Vet Publ Hlth, 25, pp.215~221 (2004)
- 하광수, "강우에 따른 적양항과 장포항 육상오염원이 창선해역에 미치는 세균학적 영향평가", 한국수산과학회지, 46(2), pp.160~167 (2013), 한국수산과학회[DOI]