In vitro Antibacterial and Synergistic Activity of an Ecklonia cava Extract against Antibiotic-Resistant Streptococcus parauberis
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
In an effort to discover alternative phytotherapeutic antimicrobial agents to combat Streptococcus parauberis, a fish pathogenic bacterium, we evaluated the antibacterial activity of seaweed extracts in vitro. A methanolic extract of Ecklonia cava exhibited strong antibacterial activity against S. parauberis isolated from olive flounder Paralichthys olivaceus. Furthermore, the n-hexane soluble (Hexane) fraction of the E. cava methanolic extract exhibited the greatest antibacterial effect on S. parauberis strains with a minimum inhibitory concentration (MIC) ranging from 256 to 1,024 μg/mL. In addition, the MIC values of oxytetracycline against antibiotic-resistant S. parauberis were markedly reduced up to 64-fold in combination with the Hexane fraction, suggesting that the antibacterial activity of the antibiotic was restored when combined with the Hexane fraction. The interaction between both antibiotics and the Hexane fraction was assessed by the fractional inhibitory concentration (FIC) index. The Hexane fraction and oxytetracycline combination against antibiotic-resistant S. parauberis strains resulted in a median ΣFIC range of 0.502 to 0.516. Thus, the synergistic ranges of median ΣFIC < 1 were observed for all combinations of the Hexane fraction and oxytetracycline against S. parauberis. To the best of our knowledge, this is the first report indicating the efficacy of an E. cava extract against fish pathogenic bacterium S. parauberis.
Abstract
In an effort to discover alternative phytotherapeutic antimicrobial agents to combat Streptococcus parauberis, a fish pathogenic bacterium, we evaluated the antibacterial activity of seaweed extracts in vitro. A methanolic extract of Ecklonia cava exhibited strong antibacterial activity against S. parauberis isolated from olive flounder Paralichthys olivaceus. Furthermore, the n-hexane soluble (Hexane) fraction of the E. cava methanolic extract exhibited the greatest antibacterial effect on S. parauberis strains with a minimum inhibitory concentration (MIC) ranging from 256 to 1,024 μg/mL. In addition, the MIC values of oxytetracycline against antibiotic-resistant S. parauberis were markedly reduced up to 64-fold in combination with the Hexane fraction, suggesting that the antibacterial activity of the antibiotic was restored when combined with the Hexane fraction. The interaction between both antibiotics and the Hexane fraction was assessed by the fractional inhibitory concentration (FIC) index. The Hexane fraction and oxytetracycline combination against antibiotic-resistant S. parauberis strains resulted in a median ΣFIC range of 0.502 to 0.516. Thus, the synergistic ranges of median ΣFIC < 1 were observed for all combinations of the Hexane fraction and oxytetracycline against S. parauberis. To the best of our knowledge, this is the first report indicating the efficacy of an E. cava extract against fish pathogenic bacterium S. parauberis.
참고문헌 (31)
- Adwan G, "Synergistic effect of plant extracts and antibiotics on Staphylococcus aureus strains isolated from clinical specimen", Middle-East J Sci Res, 3, pp.134~139 (2008)
- 백근욱, "Isolation and characterization of Streptococcus sp.from diseased flounder (Paralichthys olivaceus )in Jeju Island", Journal of Veterinary Science, 7(1), pp.53~58 (2006), 대한수의학회
- Bercovier H, "Fish Vaccinology. Dev Biol Stand", pp.153~160 (1997), Karger
- Choi JG, "Antibacterial activity of Ecklonia cava against methicillin-resistant Staphylococcus aureus and Salmonella spp", Foodborne Path Dis, 7, pp.435~441 (2010)
- Clinical and Laboratory Standards Institute, "Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard-Ninth Edition", CLSI document M07-A9, ~
- Clinical and Laboratory Standards Institute, "Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fourth Informational Supplement", CLSI document M100-S24, ~ (2014)
- Cox S, "An assessment of the antioxidant and antimicrobial activity of six species of edible Irish seaweeds", Int Food Res J, 17 (2010)
- Domenech A, "Streptococcosis in cultured turbot, Scophthalmus maximus (L.), associated with Streptococcus parauberis", J Fish Dis, 19, pp.36~38 (1996)
- Eom SH, "In vitro antibacterial activity and synergistic antibiotic effects of phlorotannins isolated from Eisenia bicyclis against methicillin-resistant Staphylococcus aureus", Phytother Res, 27, pp.1260~1264 (2013)
- Eom SH, "The mechanism of antibacterial activity of phlorofucofuroeckol-A against methicillin-resistant Staphylococcus aureus", Appl Microbiol Biotechnol, 98, pp.9795~9804 (2014)
- Gray JT, "Antibiotic susceptibility testing of bacteria isolated from animals", Clin Microbiol Newsletter, 27, pp.131~135 (2005)
- Gupta S, "Study of the microbial diversity and antimicrobial properties of Irish edible brown seaweeds", Int J Food Sci Technol, 45, pp.482~489 (2009)
- Hornsey IS, "The production of antimicrobial compounds by British marine algae I. Antibiotic-producing marine algae", Br Phycol J, 9, pp.353~361 (1974)
- Hsieh MH, "Synergy assessed by checkerboard. A critical analysis", Diagn Microbiol Infect Dis, 16, pp.343~349 (1993)
- 정용욱, "제주지역 양식넙치의 연쇄구균증 발생동향 및 원인균에 대한 분자적 동정", 미생물학회지, 42(3), pp.199~204 (2006), 한국미생물학회
- Kanagasabhapathy M, "Antibacterial activities of marine epibiotic bacteria isolated from brown algae of Japan", Ann Microbiol, 56, pp.167~173 (2006)
- Kandhasamy M, "Evaluation of in vitro antibacterial property of seaweeds of southeast coast of India", Afr J Biotechnol, 7, pp.1958~1961 (2008)
- Kang SM, "Molecular docking studies of a phlorotannin, dieckol isolated from Ecklonia cava with tyrosinase inhibitory activity", J Bioorg Med Chem, 20, pp.311~316 (2012)
- Kim JH, "Pathogenicity of Streptococcus parauberis to olive flounder Paralichthys olivaceus", Fish Pathol, 41, pp.171~173 (2006)
- Kim KN, "Protective effect of Ecklonia cava enzymatic extracts on hydrogen peroxide-induced cell damage", Process Biochem, 41, pp.2393~2401 (2006)
- 김승용, "항생제 내성 Enterococcus faecalis 에 대한 감태( Ecklonia cava ) 추출물의 항균 시너지 효과", 한국수산과학회지, 48(1), pp.51~57 (2015), 한국수산과학회
- Kolanjinathan K, "Antibacterial activity of ethanol extract of seaweeds against fish bacterial pathogens", Eur Rev Med Pharmaco Sci, 13, pp.173~177 (2009)
- 이정하, "In vitro antibacterial and synergistic effect of phlorotannins isolated from edible brown seaweed Eisenia bicyclis against acne-related bacteria", ALGAE, 29(1), pp.47~55 (2014), 한국조류학회I[DOI]
- Leigh JA, "Streptococcus uberis: A permanent barrier to the control of bovine mastitis?", Vet J, 157, pp.225~238 (2002)
- Manivannan K, "Proximate composition of different group of seaweeds from Vedalai Coastal Waters(Gulf of Mannar) : Southeast Coast of India", Middle East J Sci Res, 4, pp.72~77 (2009)
- Meletiadis J, "Defining fractional inhibitory concentration index cutoffs for additive interactions based on self-drug additive combinations, Monte Carlo simulation analysis, and in vitro-in vivo correlation data for antifungal drug combinations against Aspergillus fumigatus", Antimicrob Agents Chemother, 54, pp.602~609 (2010)
- Meng F, "Characterization of drug resistance in Streptococcus parauberis isolated from Japanese flounder", Fish Pathol, 44, pp.40~46 (2009)
- Ossiniel Nshimiyumukiza, "Synergistic Antibacterial Activity of Ecklonia cava (Phaeophyceae: Laminariales) against Listeria monocytogenes (Bacillales: Listeriaceae)", Fisheries and Aquatic Sciences, 18(1), pp.1~6 (2015), 한국수산과학회
- Park YK, "Antibiotic susceptibility and resistance of Streptococcus iniae and Streptococcus parauberis isolated from olive flounder (Paralichthys olivaceus)", Vet Microbiol, 136, pp.76~81 (2009)
- Rangaiah SG, "Antimicrobial activity of seaweeds Gracillaria, Padina and Sargassum sps. on clinical and phytopathogens", Int J Chem Anal Sci, 1, pp.114~117 (2010)
- Rhimou B, "The antibacterial potential of the seaweeds(Rhodophyceae)of the strait of gibraltar and the Mediterranean Coast of Morocco", Afr J Biotechnol, 9, pp.6365~6372 (2010)